Physiological Role of 3-Hydroxykynurenine and Xanthurenic Acid Upon Crustacean Molting
- 249 Downloads
Studies with crabs (Charybdis japonica) and crayfish (Procambarusclarkii) revealed that the tryptophan metabolites, 3-hydroxy-L-kynurenine (3-OH-K) and xanthurenic acid (XA), common secretory products of the X-organsinus gland complex of eyestalks from several decapods, regulated the molting of crustaceans in species-nonspecific fashion. Injection of 3-OH-K to the eyestalk-ablated crayfish delayed the onset of the first molt and lengthened the interval between the first and second molts. These lines of evidence were in accord with previous accounts of the so-called “molt inhibiting hormone” (MIH) effect. Removal of eyestalks caused a change in the conversion capacity of exogenous 3-OH-K to XA in the hemolymph. The peak in transformation capacity was followed by a peak in the titer of 20-hydroxyecdysone or molting hormone. Moreover, the seasonal profiles of the XA and ecdysone titers in Charybdis japonica exhibited a staggered relationship in the tissues tested. The ratio of XA to 3-OH-K, which is expected to indicate the apparent 3-OH-Kase activity, fluctuated seasonally and locally. When the Y-organ with the adhering tissues (Y-organ complex or YOC) was incubated during the period of high XA titer, the YOC produced 100 times more ecdyson than before incubation. It is suggested that ecdysteroidogenesis in situ was suppressed during this period by XA, but incubation of the YOC lead to a dramatic acceleration in ecdysone synthesis by overriding this inhibitory effect. XA profoundly repressed ecdysteroidogenesis in the YOC culture. Thus, XA is the ecdysone biosynthesis inhibitor (EBI) and 3-OH-K the precursor in crustaceans. An interfering effect of XA to a biocatalyst cytochrome P-450 system was postulated for the inhibition mechanism of ecdysteroidogenesis.
KeywordsCrude Enzyme Preparation Xanthurenic Acid Homarus Americanus Tryptophan Metabolite Sinus Gland
Unable to display preview. Download preview PDF.
- Borst, D.W., Laufer, H., Landau, M., Chang, E.S., Hertz, W.A., Baker, F.C., and Schooley, D.A., 1987, Methyl farnesoate and its role in crustacean reproduction and development, Insect Biochem., 37: 1123.Google Scholar
- Kleinholz, L.H., 1985, Biochemistry of crustacean hormones, in: “The Biology of Crustacea”, Vol. 9, Bliss, D.E., and Mantal, L.H., eds., Academic Press, Florida, pp. 463–522.Google Scholar
- Passano, L.M., 1960, Molting and its control, in: “The Physiology of Crustacea”, Vol. 1, Waterman, T.H., ed., Academic Press, New York, pp. 473–536.Google Scholar
- Skinner, D.M., 1985, Molting and regeneration, in: “The Biology of Crustacea”, Vol. 9, Bliss, D.E., and Mantel, L.H., eds., Academic Press, Florida, pp. 43–146.Google Scholar
- Smith, S.L., 1985, Regulation of ecdysteroid titer: synthesis, in: “Comprehensive Insect Physiology, Biochemistry and Pharmacology”, Kerkut, G.A., and Gilbert, L.I., eds., Pergamon Press, Oxford, pp. 295–334.Google Scholar