Abstract
The successful study of bacteriophages require a collection of various conditionally lethal mutants, preferably nonsense mutants. Nonsense mutants have a mutationally caused stop codon inside a reading frame which causes the premature termination of a peptide chain in translation. Nonsense mutants, on the other hand, can only be obtained and handled if bacterial suppressor strains are available. Such strains are characterized by a mutated t-RNA which has an alteration in the anticodon enabling it to insert an aminoacid at the nonsense codon position. Therefore, the first step in starting genetics with a phage is the search for suitable suppressor strains.
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References
Bibb, M.J, Findlay, P.R., and Johnson, M.B., 1984, The relationship between base composition in codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences. Gene, 30: 157–166.
Fournier, M.J. and Ozeki, H., 1985, Structure and organization of transfer ribonucleic acid genes of Escherichia coli K-12. Microbiol Rev, 49: 379–397.
Ikemura, T., 1981, Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational system. J. Mol. Biol., 151: 389–409.
Paradiso, M.J., Roberts, G., Streicher, S.L., and Goldberg, R.B., 1987, Characterization of suppressible mutations in the viomycin plasmid pVE138. J. Bacteriol., 169: 1325–1327.
Schmieger, H., Knerr, R., and Köberlein, M., 1988, Codon usage as a reason for unsuccessful search for amber-suppressor mutants in Streptomyces lividans? Genet. Res., 52: 163–167.
Sedlmeier, R., 1987, Charakterisierung einer Quecksilber-Resistenz aus Streptomyces lividans. Dipl. Thesis, Regensburg.
Sprinzl, M., Hartmann, T., Weber, J., Blank, J., and Zeidler, R., 1989, Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res., 17: l-172.
Vold, B.S., 1985, Strucutre and organization of genes for transfer ribonucleic acid in Bacillus subtilis. Microbiol Rev, 49: 71–80.
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© 1991 Plenum Press, New York
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Sedlmeier, R., Schmieger, H. (1991). t-RNA Genes in Streptomyces Lividans: Structure, Organization and Construction of Suppressor t-RNA. In: Baumberg, S., Krügel, H., Noack, D. (eds) Genetics and Product Formation in Streptomyces . Federation of European Microbiological Societies Symposium Series, vol 55. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5922-7_9
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DOI: https://doi.org/10.1007/978-1-4684-5922-7_9
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