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Analysis of Amplifications and Deletions in Streptomyces Species

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Genetics and Product Formation in Streptomyces

Abstract

Genetic instability is very common in Streptomyces species, and was one of the first reported properties (Beijerinck, 1913). Usually genetic instability has been detected as influencing easily scored phenotypes such as pigment production (Gregory and Huang, 1964), sporulation, auxotrophy (Redshaw et al., 1979) and antibiotic resistance (Freeman et al., 1977). In some cases genetic instability affects antibiotic production and can be a serious problem in industrial fermentations.

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References

  • Alacevic, M., Strasek-Vesligaj, M., and Sermonti, G., 1973, The circular linkage map of Streptomyces rimosus, J. Gen. Microbiol., 77: 173–185.

    Google Scholar 

  • Altenbuchner, J., and Cullum, J., 1985, Structure of an amplifiable DNA sequence in Streptomyces lividans 66, Mol. Gen. Genet., 201: 192–197.

    Article  PubMed  CAS  Google Scholar 

  • Altenbuchner, J., and Cullum, J., 1987, Amplification of cloned genes in Streptomyces, Bio/Technology, 5: 1328–1329.

    Article  CAS  Google Scholar 

  • Beijerinck, M. W., 1913, Ueber Schröter und Cohn’s Lakmusmicrococcus, Folia Microbiol., 2: 185–200.

    Google Scholar 

  • Birch, A. W., and Cullum, J., 1985, Temperature-sensitive mutants of the Streptomyces plasmid pIJ702, J. Gen. Microbiol., 131: 1299–1303.

    PubMed  CAS  Google Scholar 

  • Birch, A., Häusler, A., Vögtli, M., Krek, W., and Hütter, R., 1989, Extremely large chromosomal deletions are intimately involved in genetic instability and genomic rearrangements in Streptomyces glaucescens, Mol. Gen. Genet., 217: 447–458.

    Article  PubMed  CAS  Google Scholar 

  • Birch, A. W., 1985, Plasmid replication and recombination in Streptomyces, PhD Thesis, University of Manchester.

    Google Scholar 

  • Chen, C. W., Tsai, J. F.-Y., and Chuang, S.-E., 1987, Intraplasmid recombination in Streptomyces lividans 66, Mol. Gen. Genet., 209: 154–158.

    Article  PubMed  CAS  Google Scholar 

  • Cullum, J., Altenbuchner, J., Flett, F., and Piendl, W., 1986, DNA amplification and genetic instability in Streptomyces, Biotechnol. Gen. Eng. Rev., 4: 59–78.

    CAS  Google Scholar 

  • Danilenko, V. N., Starodubtseva, L. I., and Navashin, S. M., 1986, Regulation of expression of kanamycin resistance and chloramphenicol resistance determinants in Streptomyces lividans 66, pp. 79–81, in “Biological, Biochemical and Biomedical Aspects of Actinomycetes”, Szabo, G., Biro, S., and Goodfellow, M., eds., Akadémiai Kiadó, Budapest.

    Google Scholar 

  • Flett, F., and Cullum, J., 1987, DNA deletions in spontaneous chloramphenicol-sensitive mutants of Streptomyces coelicolor A3(2) and Streptomyces lividans 66, Mol. Gen. Genet., 207: 499–502.

    Article  PubMed  CAS  Google Scholar 

  • Freeman, R. F., Bibb, M. J., Hopwood, D. A., 1977, Chloramphenicol acetyl-transferase-independent chloramphenicol resistance in Streptomyces coelicolor A3(2), J. Gen. Microbiol., 98: 453–465.

    PubMed  CAS  Google Scholar 

  • Gregory, K. F., and Huang, J. C. C., 1964, Tyrosinase inheritance in Streptomyces scabies. I Genetic recombination, J. Bacteriol., 86: 1281–1286.

    Google Scholar 

  • Häusler, A., Birch, A., Krek, W., Piret, J., and Hütter, R., 1989, Heterogeneous genomic amplification in Streptomyces glaucescens: Structure, location and junction sequence analysis, Mol. Gen. Genet., 217: 437–446.

    Article  PubMed  Google Scholar 

  • Hranueli, D., Pigac, J., Smokvina, T., and Alacevic, M., 1983, Genetic interactions in Streptomyces rimosus mediated by conjugation and by protoplast fusion, J. Gen. Microbiol., 129: 1415–1422.

    PubMed  CAS  Google Scholar 

  • Hütter, R., Birch, A., Häusler, A., Vögtli, M., Madon, J., and Krek, W., 1988, Genome fluidity in Streptomycetes, in “Biology of Actinomycetes ‘88”, Okami, Y., Beppu, T., and Ogawara, H., eds., Japan Scientific Societies Press, Tokyo.

    Google Scholar 

  • Kendall, K., and Cullum, J., 1986, Identification of a DNA sequence associated with plasmid integration in Streptomyces coelicolor A3(2), Mol. Gen. Genet., 202: 240–245.

    Article  PubMed  CAS  Google Scholar 

  • Kendall, K., Ali-Dunkrah, U., and Cullum, J., 1987, Cloning of the galactokinase gene (galK) from Streptomyces coelicolor A3(2), J. Gen. Microbiol., 133: 721–725.

    PubMed  CAS  Google Scholar 

  • Pigac, J., and Alacevic, M., 1979, Mapping of oxytetracycline genes on Streptomyces rimosus chromosome, Period biol, 81: 575–582.

    Google Scholar 

  • Pigac, J., Hranueli, D., Smokvina, T., and Alacevic, M., 1982, Optimal cultural and physiological conditions for handling Streptomyces rimosus protoplasts, Appl. Environ. Microbiol., 44: 1178–1186.

    PubMed  CAS  Google Scholar 

  • Pigac, J., Vujaklija, D., and Gamulin, V., 1986, Structural segregation of a bifunctional vector pZG1 in Streptomyces lividans and S. rimosus, in “Biological, Biochemical and Biomedical Aspects of Actinomycetes”, Szabo, G., Biro, S., and Goodfellow, M., eds., Akadémiai Kiadó, Budapest.

    Google Scholar 

  • Pigac, J., Vujaklija, D., Toman, Z., Gamulin, V., and Schrempf, H., 1988, Structural instability of a bifunctional plasmid pZG1 and singlestranded DNA formation in Streptomyces, Plasmid, 19: 222–230.

    Article  PubMed  CAS  Google Scholar 

  • Redshaw, P. A., McCann, P..A., Pentella, M..A., and Pogell, B. M., 1979, Simultaneous loss of multiple differentiated features in aerial mycelium-negative isolates of Streptomyces, J. Bacteriol., 137: 891–899.

    PubMed  CAS  Google Scholar 

  • Zhou, X., Deng, Z., Firmin, J. L., Hopwood, D. A., and Kieser, T., 1988, Site-specific degradation of Streptomyces lividans DNA during electrophoresis in buffers contaminated with ferrous iron, Nucleic Acids Res., 16: 4341–4352.

    Article  PubMed  CAS  Google Scholar 

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© 1991 Plenum Press, New York

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Cullum, J. et al. (1991). Analysis of Amplifications and Deletions in Streptomyces Species. In: Baumberg, S., Krügel, H., Noack, D. (eds) Genetics and Product Formation in Streptomyces . Federation of European Microbiological Societies Symposium Series, vol 55. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5922-7_31

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  • DOI: https://doi.org/10.1007/978-1-4684-5922-7_31

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5924-1

  • Online ISBN: 978-1-4684-5922-7

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