Summary
Streptomyces viridochromogenes Tü494, the producer of phosphinothricyl-alanyl-alanine (Ptt), is sensitive to its own antibiotic. Two phenotypically discernible Ptt-resistant S. viridochromogenes mutants, ES1 and ES2, were isolated and employed to clone resistance genes. Thus, two different DNA fragments both conferring Ptt resistance could be detected. The DNA regions including the resistance genes were sequenced and the gene products were investigated. The first gene (pat) encodes a phosphinothricin N-acetyltransferase which inactivates the antibiotically effective component phosphinothricin. Following modification of the 5’ region, the pat gene was transferred into plants and phosphinothricin-resistant transgenic plants were obtained. The second gene, a glutamine synthetase (GS) gene mediated Ptt resistance in multi-copy state only. The gene product is heat-labile, and the deduced amino acid sequence was shown to be highly homologous to eucaryotic and to Rhizobiaceae GSII-type enzymes. Therefore the gene was named glnII. Southern hybridizations with different Streptomyces strains suggest that they all carry two types of GS genes, g1nA and g1nII.
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© 1991 Plenum Press, New York
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Wohlleben, W. et al. (1991). Genetic Analysis of Different Resistance Mechanisms Against the Herbicidal Antibiotic Phosphinothricyl-Alanyl-Alanine. In: Baumberg, S., Krügel, H., Noack, D. (eds) Genetics and Product Formation in Streptomyces . Federation of European Microbiological Societies Symposium Series, vol 55. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5922-7_20
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DOI: https://doi.org/10.1007/978-1-4684-5922-7_20
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