Summary
27 actinophages of Saccharopolyspora (formerly Faenia) rectivirgula, Sap. hirsuta, Sap. hordei and Sap. erythraea were compared with respect to host range, genome length and G+C composition, virion morphology and DNA homology. According to the rules of the ICTV it was thus possible to allocate the phages to 8 new bacteriophage species. Interspecific DNA hybridization indicated a modular organization of the phage genomes: some DNA regions, presumably modules, are distributed independently among many of the phage species described. For further studies of such modules different approaches were chosen: (1) the A-“module” was used to integrate a thiostrepton resistance gene (tsr) by homologous recombination into the prophages of øFR113 and øFR755R to be able to monitor module exchange in future studies. (2) Several virulent mutants of the temperate øFRb-D have lost the same short DNA region; the corresponding fragments of the wild-type phage, a deletion mutant and a virulent mutant without deletions were cloned for further analysis. (3) The restriction maps of øFRb-D and øFRb-P are presented; both phages are closely related but øFRb-P has a broader host-range among Saccharoployspora than øFRb-D.
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© 1991 Plenum Press, New York
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Schneider, J. (1991). Characterization of Eight Groups (Species) of Saccharopolyspora Phages — An Example for Studying Modular Organization of Actinophage Genomes. In: Baumberg, S., Krügel, H., Noack, D. (eds) Genetics and Product Formation in Streptomyces . Federation of European Microbiological Societies Symposium Series, vol 55. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5922-7_10
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DOI: https://doi.org/10.1007/978-1-4684-5922-7_10
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