Abstract
Candida alhicans has proved to be a difficult organism to study in terms of its genetics and molecular biology; which is a pity, since the power of molecular genetic methods can greatly enhance the scientific quality of many experiments with the fungus. Because C.alhicans is diploid and has no natural sexual cycle, traditional approaches to mutation and genetic mapping have yielded few insights into the properties of its DNA. However, this position is at last beginning to change rapidly. Genes that code for products of importance in the study of C.albicans morphogenesis, responses to antifungal chemotherapy and the pathogenesis of candidosis are now being successfully cloned, and methods of DNA typing have been applied to epidemiological studies of candidosis. The future for molecular genetic approaches to Candida-related problems is a bright one. This brief overview will amount to a summary of the present state of the molecular genetic art in the context of Candida and Candida infections, with consideration of the problems where DNA-manipulative experimentation can be most usefully applied.
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References
W.L. Whelan and P.T. Magee,Natural heterozygosity in Candida albicans, J. Bacteriol., 145: 896 (1981).
W.S. Riggsby, L.J. Torres-Bauza, J.W. Wills, T.M. Townes, DNA content, kinetic complexity, and the ploidy question in Candida albicans, Mol. Cell Biol, 2: 853 (1982).
R. Poulter, V. Hanrahan, K. Jeffery, D. Markie, M.G. Shepherd, P.A. Sullivan, Recombination analysis of naturally diploid Candida albicans, J.Bacteriol., 152: 969 (1982).
A.M.Gillum, E.Y.H. Tsay, D.R. Kirsch, Isolation of the Candida albicans gene for orotidine-5’-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E.coli pyrF mutations, Mol.Gen.Genet., 198: 179 (1984).
P.T.Magee, E.H.A. Rikkerink, B.B.Magee, Review methods for the genetics and molecular biology of Candida albicans, Anal. Biochem., 175: 361 (1988).
M.B.Kurtz, M.W. Cortelyou, D.R. Kirsch, Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene, Mol. Cell Biol., 6: 142 (1986).
M.B. Kurtz, M.W. Cortelyou, S.M. Miller, M. Lai, D.R. Kirsch, Development of autonomously replicating plasmids for Candida albicans, Mol. Cell Biol., 7: 209 (1987).
R.Kelly, S.M.Miller, M.B. Kurtz, D.R. Kirsch, Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants, Mol. Cell Biol., 7: 199 (1987).
R. Kelly, S.M. Miller, M.B. Kurtz, One-step gene disruption by contransformation to isolate double auxotrophs in Candida albicans, Mol. Gen. Genet., 214: 24 (1988).
R.G.Snell, R.J. Wilkins, Separation of chromosomal DNA molecules from C. albicans in pulsed field gel electrophoresis, Nucl. Acid Res., 14: 4401 (1986).
B.B. Magee, Y. Koltin, J.A. Gorman, P.T. Magee, Assigment of cloned genes to the seven electrophoretically separated Candida albicans chromosomes, Mol.Cell Biol., 8: 4721 (1988).
D.R.Soll, C.J.Langtimm, J.McDowell, J.Hicks, R.Galask, High-frequency switching in Candida strains isolated from vaginitis patients, J.Clin. Microbiol., 25: 1611 (1987).
S.Scherer, D.A. Stevens, A Candida albicans dispersed, repeated gene family and its epidemiologic applications, Proc.Nat.Acad.Sci. (USA), 85: 1452 (1988).
J.E.Culter, P.M.Glee, H.L.Horn, Candida albicans and Candida stellatoidea-specific DNA fragment, J. Clin. Microbiol., 26: 1720 (1988).
D.R. Solly, M. Staebell, C. Longtimm, M. Pfaller, J. Hicks, T.V. Gopala Rao, Multiple Candida strains in the course of a single systemic infection, J. Clin, Microhiol., 26: 1448 (1988).
M.M.Mason, B.A.Lasker, W.S.Riggsby, Molecular probe for identification of medically important Candida species and Torulopsis glabrata, J. Clin.Microbiol., 25: 563 (1987).
B.B.Magee, T.M. D’Souza, P.T. Magee, Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species, J.Bacteriol., 169: 1639 (1987).
B.B. Magee and P.T. Magee, Electrophoretic karyotypes and chromosome number in Candida species, J.Gen.Microbiol., 133: 425 (1987).
P.D.Olivo, E.J. McManus, W.S. Riggsby, J.M. Jones, Mitochondrial DNA polymorphism in Candida albicans, J.Infect.Dis., 156: 214 (1987).
W.L. Whelan, E.Reiss, B.B.Magee, B.L. Wickes, Genetic differences between type I and type II Candida stellatoidea, Infect. Immun., 57: 527 (1989).
B.C.Fox, H.L.T. Mobley, J.C. Wade, The use of a DNA probe for epidemiological studies of candidiasis in immunocompromised hosts. J.Infect. Dis., 159: 488 (1989).
S.Scherer and D.A. Stevens, Application of DNA typing methods to the epidemiology and taxonomy of Candida species, J.Clin. Microbiol, 25: 675 (1987).
R.Matthews and J. Barnie, Assessment of DNA fingerprinting for rapid identification of outbreaks of systemic candidiasis, Br.Med. J., 298: 354 (1989).
T.J.Lott, P. Boiron, E. Reiss, An electrophoretic karyotype for Candida albicans reveals large chromosomes in multiples, Mol.Gen. Genet., 209: 170 (1987).
R.G.Snell, I.F. Hermans, R.J.Wilkins, B.E.Corner, Chromosomal variations in Candida albicans,Nuc1.Acid Res., 15: 3625 (1987).
K.J. Kwon-Chung, B.L. Wickes, W.G. Merz, Association of electrophoretic karyotype of Candida stellatoidea with virulence for mice, Infect. Immun., 56: 1814 (1988).
W.G. Merz, C.Connelly, P. Hieter, Variation of electrophoretic karyotypes among clinical isolates of Candida albicans, J. C.in. Microbiol, 26: 842 (1988).
T.J. Lott, L.S. Page, P. Boiron, J. Benson, E. Reiss, Nucleotide sequence of the Candida albicans aspartyl proteinase gene, Nucl Acid Res., 17: 1779 (1989).
P.J. Russell, J.A. Welsch, E.M. Rachlin, J.A. McCloskey, Different levels of DNA methylation in yeast and mycelial forms of Candida albicans, J.Bacteriol., 169: 4393 (1987).
D.R.Kirsch, M.H. Lai, J. O’Sullivan, Isolation of the gene for cytochrome P450 L1A1 (lanosterol 14a-demethylase) from Candida albicans, Gene, 68: 229 (1988).
M.H.Lai and D.D.Kirsch, Nucleotide sequence of cytochrome P450 L1A1 (lanosterol 14a-demthylase) from Candida albicans, Nucl. Acid. Res., 17: 804 (1989).
S.C. Singer, C.A. Richards, R.Ferone, D. Benedict, P. Ray, Cloning, purification and properties of Candida albicans thymidylate synthase, J.Bacteriol., 171: 1372 (1989).
A. Rosenbluh, M. Mevarech, Y. Koltin, J.A. Gorman, Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae, Mol. Gen. Genet., 200: 500 (1985).
M.B. Kurtz, D.R. Kirsch, R. Kelly, The molecular genetics of Candida albicans, Microbiol. Sci., 5: 58 (1988).
H.F. Jenkinson, G.P. Schep, M.G. Shepherd, Cloning and expression of the 3-isopropylmalate dehydrogenase gene from Candida albicans, FEMS Microbiol. Lett., 49: 285 (1988).
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© 1991 Plenum Press, New York
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Odds, F.C. (1991). Perspective of the Application of Molecular Biology Techniques in the Study of Candida . In: Tümbay, E., Seeliger, H.P.R., Anǧ, Ö. (eds) Candida and Candidamycosis. Federation of European Microbiological Societies Symposium Series, vol 50. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5910-4_2
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DOI: https://doi.org/10.1007/978-1-4684-5910-4_2
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