Abstract
The application of chemical kinetic investigations, using fast reaction techniques, to the studies of neuronal receptor-mediated reactions has been recently reported (Hess et al., 1979, 1987). However, the use of such mixing techniques is limited to the studies of receptors in membrane vesicles (Hess et al., 1979) with a time resolution of 5 msec, or to measurements with receptor-containing cells (Udgaonkar and Hess, 1987) with a 20 msec time resolution. In order to clarify the activation process of receptors, a new method which has a faster time resolution must be developed. Caged compounds pioneered by Professor Jack Kaplan, University of Pennsylvania, and Professor David Trentham, National Institute for Medical Research, London, are good candidates for this purpose. In contrast to their oxygen-bonded caged compounds, we protected nitrogen atoms included in many neurotransmitters. We have synthesized caged neurotransmitters bonded to a photo-dissociative o-nitrobenzyl group with the nitrogen atom. These compounds have shown relatively fast photolysis and have been useful for the study of neuronal receptors.
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References
Hess, G.P., Cash, D.J., and Aoshima, H. (1979). Acetylcholine receptor-controlled ion fluxes in membrane vesicles investigated by fast reaction techniques. Nature (London) 282, 329–331.
Hess, G.P., Udgaonkar, J.B., and Olbricht, W.L. (1987). Chemical kinetic measurements of transmembrane processes using rapid reaction techniques: Acetylcholine receptor. Ann. Rev. Biophys. Biophys. Chem. 16, 507–533.
McCray, J.A., Herbette, L., Kihara, T., and Trentham, D.R. (1980). A new approach to time-resolved studies of ATP-requiring biological systems: Laser flash photolysis of caged ATP. Proc. Natl. Acad. Sci. U.S.A. 77, 7237–7241.
Milburn, T., Matsubara, N., Billington, A.P., Udgaonkar, J.B., Walker, J.W., Carpenter, B.K., Webb, W.W., Marque, J., Denk, W., McCray, J.A. and Hess, G.P. (1989). Synthesis, photochemistry, and biological activity of a caged phtrtolabile acetylcholine receptor ligand. Biochemistry 28, 49–55.
Udgaonkar, J.B., and Hess, G.P. (1986). Acetylcholine receptor kinetics: Chemical kinetics. J. Membr. Biol 93, 93–109.
Udgaonkar, J.B., and Hess, G.P. (1987). Chemical kinetic measurements of a mammalian acetylcholine receptor by a fast-reaction technique. Proc. Natl. Acad. Sci. U.S.A. 84, 8758–8762.
Walker, J.W., McCray, J.A., and Hess, G.P. (1986). Photolabile protecting groups for an acetylcholine receptor ligand. Synthesis and photochemistry of a new class of onitrobenzyl derivatives and their effects on receptor function. Biochemistry 25, 1799–1805.
Wettermark, G. (1962). Photochromism of o-nitrotoluenes. J. Phys. Chem. 66, 2560–2562.
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© 1991 Plenum Press, New York
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Matsubara, N. et al. (1991). Chemical Kinetic Investigations of the Channel-Opening Process of Neurotransmitter Receptors. In: Kito, S., Segawa, T., Olsen, R.W. (eds) Neuroreceptor Mechanisms in Brain. Advances in Experimental Medicine and Biology, vol 287. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5907-4_7
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DOI: https://doi.org/10.1007/978-1-4684-5907-4_7
Publisher Name: Springer, Boston, MA
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