Considerations in Designing and Analyzing Data from Apo-B Turnover Studies

  • David M. Foster
  • P. Hugh
  • R. Barrett
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 285)


The kinetics of apo-B transport through VLDL, IDL and LDL can be described by models developed from tracer data (1,2). From these models, one obtains estimates of metabolic parameters of interest such as (i) fractional catabolic rates, (ii) production rates, and (iii) transport from one lipoprotein class to another. The models, however, have assumptions built into them which affect the interpretation of the data and parameter estimates. With many new experimental procedures being developed, our knowledge of the structure and function of the plasma lipoproteins is rapidly increasing. Many of these new procedures lend themselves to turnover studies with the result that tracer data of increasing complexity are being generated. These include techniques to subfractionate the traditional lipoprotein populations (3,4) and the utilization of stable as well as radioactive isotopes (5,6). The interpretation of these data means that many of the previously developed models may have to be altered or changed altogether as the assumptions upon which they were based prove no longer to be valid. With tracer studies being used to elucidate altered metabolic pathways in pathophysiological conditions or in response to therapy, one runs a high risk of misinterpreting turnover data using models with underlying false assumptions.


Isotopic Enrichment Tracer Data Fractional Catabolic Rate Kinetic Heterogeneity Familial Combine Hyperlipidemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    M. Berman. Kinetic analysis of turnover data. Prog. Biochem. Pharmacol. 15:67 (1979).PubMedGoogle Scholar
  2. 2.
    M. Berman, S. M. Grundy and B. V. Howard, eds. Lipoprotein Kinetics and Modeling, Academic Press, New York (1982).Google Scholar
  3. 3.
    C. A. Marzetta, D. M. Foster, and J. D. Brunzell. Relationships between LDL density and kinetic heterogeneity in subjects with normolipidemia and familial combined hyperlipidemia using density gradient ultracentrifugation. J. Lipid Res. 30:1307 (1989).PubMedGoogle Scholar
  4. 4.
    E. Trezzi, C. Calvi, P. Roma, and A. L. Catapano. Subfractionation of human very low density lipoproteins by heparin-Sepharose affinity chromatography. J. Lipid Res. 24:790 (1983).PubMedGoogle Scholar
  5. 5.
    D. R. Cryer, T. Matsushima, J. B. Marsh, M. Yudkoff, P. M. Coates, and J.A. Cortner. Direct measurement of apolipoprotein B synthesis in human very low density lipoprotein using stable isotopes and mass spectrometry. J. Lipid Res. 27:508 (1986).PubMedGoogle Scholar
  6. 6.
    J. S. Cohn, D. A. Wagner, S. D. Cohn, J. S. Millar, and E. J. Schaefer. The measurement of very low density and low density lipoprotein apoB-100 and high density lipoprotein apoA-1 synthesis in human subjects using deuterated leucine: effect of fasting and feeding. J. Clin. Invest. 85:804 (1990).PubMedCrossRefGoogle Scholar
  7. 7.
    R. A. Shipley and C. E. Clark. Tracer Methods for In Vivo Kinetics: Theory and Application, Academic Press, New York (1972).Google Scholar
  8. 8.
    D. M. Foster, A. Chait, J.J .Albers, C. Harris and J.D. Brunzell. Evidence for kinetic heterogeneity among human low density lipoproteins. Metabolism. 35:685 (1986).PubMedCrossRefGoogle Scholar
  9. 9.
    R. S. Kushwaha, D. M. Foster, P. H. R. Barrett, and K. D. Carey. Effect of estrogen and progesterone on the metabolism of apoprotein B in baboons. Am. J. Phvsiol. 258: E172 (1990)Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • David M. Foster
    • 1
  • P. Hugh
    • 1
  • R. Barrett
    • 1
  1. 1.Center for Bioenginecring, FL-20University of WashingtonSeattleUSA

Personalised recommendations