Regression of Atheroma and Putative Role of CETP in Cholesteryl Ester Removal

  • Yechezkiel Stein
  • Olga Stein
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 285)


Evidence for regression of atherosclerosis induced by cholesterol feeding has been provided by several investigators [1–3]. However, since the extent of atherosclerotic involvement is quite variable, quantitative evaluation of regression is difficult. We have used 3H-cholesteryl linoleyl ether (3H-CLE), a nonhydrolyzable analog of cholesteryl easter as a stable marker for the quantitation of atherosclerotic involvement [4] and evaluated the potential usefulness of 3H-CLE in the evaluation of regression of atheromatosis [5]. To that end, 20 rabbits were kept on a purina diet enriched with 1% cholesterol for 1 month and then on alternate weeks for an additional 2 months. The animals were randomized into two groups according to their plasma cholesterol levels and injected with autologous plasma labeled with 3H-CLE [5]. The baseline group was killed 10–12 days after injection, while the regression group was fed purina fortified with 3% cholestyramine and killed 8–11 months after injection of the 3H-CLE. We investigated the following: 1. Will the 3H-CLE remain in the aorta during the 11-month period of regression? 2. If 3H-CLE is retained, then the specific activity expressed as 3H-CLE/CE mass should rise with CE loss during regression; 3. Is the loss of CE during regression similar from the different parts of the aorta?


Cholesteryl Ester Regression Group Baseline Group Cholesterol Feeding Atherosclerosis Regression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D.Vesselinovitch, R.W.Wissler, K.Fisher-Dzoga, R.Hughes, and L.Dubien, Regression of atherosclerosis in rabbits. Part 1. Treatment with low-fat diet, hyperoxia and hypolipidemic agents, Atherosclerosis19:259 (1974).PubMedCrossRefGoogle Scholar
  2. 2.
    R.W.St.Clair, Atherosclerosis regression in animal models: Current concepts of cellular and biochemical mechanisms, Prog.Cardiovasc. Pis. 26: 109 (1983).CrossRefGoogle Scholar
  3. 3.
    M.R.Maiinow, Experimental models of atherosclerosis regression. Atherosclerosis 48: 105 (1983).CrossRefGoogle Scholar
  4. 4.
    Y.Stein, O.Stein, and G.Halperin, Use of 3H-cholesteryl linoleyl ether for the quantitation of plasma cholesteryl ester influx into the aortic wall in hypercholesterolemic rabbits, Arteriosclerosis2:281 (1982).PubMedCrossRefGoogle Scholar
  5. 5.
    O.Stein, G.Hollander, Y.Dabach, G.Halperin, and Y.Stein, Use of 3H-cholesteryl linoleyl ether as a quantitative marker for loss of cholesteryl ester during regression of cholesterol-induced aortic atheromas in rabbits, Arteriosclerosis 9:247 (1989).PubMedCrossRefGoogle Scholar
  6. 6.
    O.Stein, G.Halperin, and Y.Stein, Cholesteryl ester efflux from extra cellular and cellular elements of the arterial wall. Model systems in culture with cholesteryl linoleyl ether. Arteriosclerosis 6:70 (1986).PubMedCrossRefGoogle Scholar
  7. 7.
    R.E.Morton, Interaction of plasma-derived lipid transfer protein with macrophages in culture, J.Lipid Res. 29:1367 (1988).PubMedGoogle Scholar
  8. 8.
    Y.-S.C.Son and D.B.Zilversmit, Increased Lipid Transfer Activities in hyperlipidemic rabbit plasma, Arteriosclerosis, 6:345 (1986).PubMedCrossRefGoogle Scholar
  9. 9.
    D.K.Spady, and J.M.Dietschy, Dietary saturated triacylglycerols suppress hepatic low density lipoprotein receptor activity in the hamster, Proc.Natl.Acad.Sci.USA, 82: 4526 (1985).PubMedCrossRefGoogle Scholar
  10. 10.
    D.K.Spady, and J.M.Dietschy, Interaction of dietary cholesterol and triglycerides in the regulation of hepatic low density lipoprotein transport in the hamster, J.Clin.Invest. 81:300 (1988).PubMedCrossRefGoogle Scholar
  11. 11.
    Y.Stein, Y.Dabach, G.Hollander, and O.Stein, Cholesteryl ester transfer activity in hamster plasma: increase by fat and cholesterol rich diets, Biochim.Biophys.Acta 1042: 138 (1990).PubMedGoogle Scholar
  12. 12.
    Y.Stein, Y.Kleinman, G.Halperin, and O.Stein, Hepatic retention and elimination of cholesteryl linoleyl ether after injection of labeled acetylated LDL or chylomicrons, Biochim.Biophys.Acta750:300 (1983).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Yechezkiel Stein
    • 1
    • 2
  • Olga Stein
    • 1
    • 2
  1. 1.Lipid Research Laboratory, Department of Medicine BHadassah University HospitalJerusalemIsrael
  2. 2.Department of Experimental Medicine and Cancer ResearchHebrew University-Hadassah Medical SchoolJerusalemIsrael

Personalised recommendations