Metabolic Role of Human Apoprotein A-IV

  • L. Lagrost
  • P. Gambert
  • A. Athias
  • C. Lallemant
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 285)


Compared with the other human apoproteins, physiological variations and metabolic functions of apo A-IV remain obscure. The apo A-IV has been discovered in rat HDL and has been later found in human plasma, mesenteric lymph and interstitial fluid. Apo A-IV is synthetized by intestine and liver and is catabolized by liver and kidneys. As apo A-IV has been suspected to play a role in triglyceride transport, we undertook the study of its plasma concentration in a population of subjects with various plasma triglyceride levels. Moreover, recent studies brought some evidence for an implication of apo A-IV in the reverse cholesterol transport. Because recent reports suggested that HDL was the major lipoprotein class involved in this metabolic pathway, we investigated the structural and metabolic relation between apo A-IV and HDL.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    L. Lagrost, P. Gambert, S. Meunier, P.Morgado, J. Degres, P. d’Athis, and C. Lallemant, Correlation between apolipoprotein A-IV and triglyceride concentrations in human sera, J. Lipid Res., 30:701 (1989).PubMedGoogle Scholar
  2. 2.
    P. H. Green, R.P. Glickman, J.W. Riley, and E. Quinet, Human apolipoprotein A-IV : intestinal origin and distribution in plasma, J. Clin. Invest., 65:911 (1980).PubMedCrossRefGoogle Scholar
  3. 3.
    C. L. Bisgaier, O. P. Sachdev, I. L. Megna, and R. M. Glickman, Distri bution of apolipoprotein A-IV in human plasma, J. Lipid Res., 26:11 (1985).PubMedGoogle Scholar
  4. 4.
    P. J. Nestel, N. H. Fidge, and M. H. Tan, Increased lipoprotein-remnant formation in chronic renal failure, N. Engl. J. Med., 307:329 (1982).PubMedCrossRefGoogle Scholar
  5. 5.
    M. Seishira, and Y. Muto, An increased apo A-IV serum concentration of patients with chronic renal failure on hemodialysis, Clin. Chim. Acta, 167:303 (1987).CrossRefGoogle Scholar
  6. 6.
    N. A. Elshourbagy, M. S. Boguski, W. S. L. Liao, L. S. Jefferson, J. I. Gordon, and J. M. Taylor, Expression of rat apolipoprotein A-IV and A-I genes : mRNA induction during development and in response to glucocorticoids and insulin, Proc. Natl. Acad. Sci. USA, 82:8242 (1985).PubMedCrossRefGoogle Scholar
  7. 7.
    M. Pessah, C. Salvat, S. R. Wang, and R. Infante, In vitro synthesis of apo A-IV and apo C by liver and intestinal MRNAS from lean and obese Zucker rats. Biochem. Biophys. Res. Commun, 142:78 (1987).PubMedCrossRefGoogle Scholar
  8. 8.
    M. Rosseneu, G. Michiels, W. De Keersgieter, J. Bury, J. P. De Slypere, H. Dieplinger, and G. Utermann, Quantification of human apolipoprotein A-IV bysandwich-type enzyme-linked immunosorbent assay, Clin. Chem., 34:739 (1988).PubMedGoogle Scholar
  9. 9.
    G. Utermann, and U. Beisiegel, Apolipoprotein A-IV : a protein occurring in human mesenteric lymph chylomicron and free in plasma. Isolation and quantification, Eur. J. Biochem., 99:333 (1979).PubMedCrossRefGoogle Scholar
  10. 10.
    L. Lagrost, P. Gambert, M. Boquillon, and C. Lallemant, Evidence for high density lipoprotein as the major apolipoprotein A-IV containing fraction in normal human serum, J. Lipid Res., 30:1525 (1989).PubMedGoogle Scholar
  11. 11.
    P. Gambert, L. Lagrost, A. Athias, S. Bastiras, and C. Lallemant, Role of apolipoprotein A-IV in the interconversion of HDL subclasses, Advances in Experimental Medicine and Biology, 243:263 (1988).PubMedCrossRefGoogle Scholar
  12. 12.
    P. J. Barter, G. J. Hopkins, and L. Gorjatschko, Lipoprotein substrates for plasma cholesterol esterification. Influence of particle size and composition of the high-density lipoprotein subfraction 3, Atherosclerosis, 58:97 (1985).PubMedCrossRefGoogle Scholar
  13. 13.
    A. Steinmetz, and G. Utermann, Activation of lecithin : cholesterol acyltransferase by human apolipoprotein A-IV, J. Biol. Chem., 260:2258 (1985).PubMedGoogle Scholar
  14. 14.
    C. H. Chen, and J. J. Albers, Activation of lecithin : cholesterol acyltransferase by apolipoprotein E-2, E-3, and A-IV isolated from plasma, Biochim. Biophys. Acta, 836:279 (1985)PubMedGoogle Scholar
  15. 15.
    E. Dvorin, N. L. Gorder, D. M. Benson, and A. M. Gotto, Jr., Apolipoprotein A-IV. A determinant for binding and uptake of high density lipoproteins by rat hepatocytes, J. Biol. Chem., 261:15714 (1986).PubMedGoogle Scholar
  16. 16.
    Y. B. Mitchel, V. A. Rifici, and H. A. Eder, Characterization of the specific binding of rat apolipoprotein E-deficiency HDL to rat hepatic plasma membranes, Biochim. Biophys. Acta, 917:324 (1987).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • L. Lagrost
    • 1
  • P. Gambert
    • 1
  • A. Athias
    • 1
  • C. Lallemant
    • 1
  1. 1.Laboratoire de Biochimie des LipoproteinesHôpital du BocageDijon CedexFrance

Personalised recommendations