Abstract
Myoblast therapy, or the transfer of normal myoblasts into dystrophic muscle, requires both a large number and high purity of human myoblasts. Solutions to both of these problems are presented below.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Hauschka, S.D., Clonal analysis of vertebrate myogenesis II. Environmental influences upon human muscle differentiation, Dev. Biol. 37:329 (1974).
Hauschka, S.D., Clonal analysis of vertebrate myogenesis. 3. Developmental changes in the muscle-colony-forming cells of the human fetal limb, Dev. Biol. 37:345 (1974).
Blau, H.M. and C. Webster, Isolation and characterization of human muscle cells, Proc. Natl. Acad. Sci. USA 78: 5623 (1981).
Blau, H.M., I. Kaplan, T.-W. Tao and J.P. Kriss, Thyroglobulin-independent, cell-mediated cytotoxicity of human eye muscle cells in tissue culture by lymphocytes of a patient with Graves’ ophthalmopathy, Life Sci. 32: 45 (1982).
Blau, H.M., C. Webster, C.-P. Chiu, S. Guttman and F. Chandler, Differentiation properties of pure populations of human dystrophic muscle cells, Exp. Cell Res. 744:495 (1983).
Blau, H.M., C. Webster and G.K. Pavlath, Defective myoblasts identified in Duchenne muscular dystrophy, Proc. Natl. Acad. Sci. USA 80:4856 (1983).
Blau, H.M., G.K. Pavlath, E.C. Hardeman, C.-P. Chiu, L. Silberstein, S.G. Webster, S.C. Miller and C. Webster, Plasticity of the differentiated state, Science 230: 758 (1985).
Costa, E.M., H.M. Blau and D. Feldman, 1,25 dihydroxyvitamin D3 receptors and hormonal responses in cloned human skeletal muscle cells, Endocrin. 119: 2214 (1986).
Hardeman, E., C.-P. Chiu, A. Minty and H.M. Blau, The pattern of actin expression in human fibroblast X mouse muscle heterokaryons suggests that human muscle regulatory factors are produced, Cell, 47: 123 (1986).
Kaplan, I.K. and H.M. Blau, Metabolic properties of human acetylcholine receptors can be characterized on cultured human muscle, Exp. Cell Res. 166:379 (1986).
Shimizu, M., C. Webster, D.O. Morgan, H.M. Blau and R.A. Roth, Insulin and insulin-like growth factor receptors and responses in cultured human muscle cells, Amer. J. Phvsiol. 251:E611 (1986).
Webster, C., G. Filippi, A. Rinaldi, C. Mastopaolo, M. Tondi, M. Siniscalco, and H.M. Blau, The myoblast defect identified in Duchenne muscular dystrophy is not a primary expression of the DMD mutation: Clonal analysis of myoblasts from double heterozygotes for two X-linked loci: DMD and G6PD, Human Genetics 74: 74 (1986).
Gunning, P., E. Hardeman, R. Wade, P. Ponte, W. Bains, H.M. Blau and L. Kedes, Differential patterns of transcript accumulation during human myogenesis, Mol. Cell Biol., 7:4100 (1987).
Miller, S.C., H.Ito, H.M. Blau and F.M. Torti, Tumor necrosis factor inhibits human myogenesis in vitro, Mol. Cell Biol. 8: 2295 (1988).
Webster, C., G.K. Pavlath, F.S. Walsh and H.M. Blau, Isolation of human myoblasts with the fluorescence-activated cell sorter, Exp. Cell Res. 174:25 (1988).
Webster, C. and H.M. Blau, Extensive proliferative capacity of normal postnatal human myoblasts, 1989 submitted.
Yasin, R., F.S. Walsh, D.N. Landon and E.J. Thompson, New approaches to the study of human dystrophic muscle cells in culture, J. Neurol. Sci. 58:315 (1983).
Miranda, A.F., T. Mongini, and S. DiMauro, Human myopathies in muscle culture: Morphological, cytochemical, and biochemical studies, Adv. Cell Culture. 4:1 (1985).
Martinuzzi, A., V. Askanas, T. Kobayashi, W.K. Engel and S. DiMauro, Expression of muscle-gene-specific isozymes of phosphorylase and creatine kinase in innervated cultured human muscle, J. Cell Biol. 103:1423 (1986).
Ham, R.G., J.A. St.Clair, C. Webster and H.M. Blau, Improved media for normal human muscle satellite cells: serum-free clonal growth with low serum, In Vitro Cell. Dev. Biol. 24:833 (1988).
Hurko, O., E.P. Hoffman, L. McKee, D.R. Johns and L.M. Kunkel, Dystrophin analysis in clonal myoblasts derived from a Duchenne muscular dystrophy carrier, Amer. J. Hum. Genet. 44:820 (1989).
Walsh, F.S., G. Dickson, S. Moore and C.H. Barton, Unmasking N-CAM, Nature 339: 516 (1989).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this chapter
Cite this chapter
Blau, H.M., Webster, C., Pavlath, G.K. (1990). Purification and Proliferation of Human Myoblasts Isolated with Fluorescence Activated Cell Sorting. In: Griggs, R.C., Karpati, G. (eds) Myoblast Transfer Therapy. Advances in Experimental Medicine and Biology, vol 280. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5865-7_12
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5865-7_12
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5867-1
Online ISBN: 978-1-4684-5865-7
eBook Packages: Springer Book Archive