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Postlabeling Analysis of Polycyclic Aromatic Hydrocarbon: DNA Adducts in White Blood Cells of Foundry Workers

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Book cover Genetic Toxicology of Complex Mixtures

Part of the book series: Environmental Science Research ((ESRH,volume 39))

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Abstract

Blood samples were obtained from 61 volunteers working in a Finnish iron foundry who were occupationally exposed to polycyclic aromatic hydrocarbons (PAHs) from 19 control subjects not known to be occupationally exposed to this class of chemical carcinogens. Foundry workers were classified as belonging to high, medium, or low exposure groups according to their exposure to airborne benzo(a)pyrene (BaP) (high > 0.2, medium 0.05–0.2, low < 0.05 µg BaP/m3 air). Aromatic adducts were determined in white blood cell DNA from the subjects using the 32P-postlabeling technique. There was a high correlation between the estimated exposure and adduct levels.

Differences in the level of adducts were also noted between job categories in the foundry. This study suggests that the 32P-postlabeling assay may be useful in monitoring human exposure to known and previously unidentified environmental genotoxic agents.

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Author information

Authors and Affiliations

  1. Institute of Occupational Health, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland

    K. Hemminki

  2. Department of Pharmacology, Baylor College of Medicine, Houston, Texas, 77030, USA

    K. Randerath & M. V. Reddy

Authors
  1. K. Hemminki
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  2. K. Randerath
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  3. M. V. Reddy
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Editor information

Editors and Affiliations

  1. US Environmental Protection Agency, Research Triangle Park, North Carolina, USA

    Michael D. Waters , Joellen Lewtas , Martha M. Moore  & Stephen Nesnow , ,  & 

  2. US Environmental Protection Agency, Cincinnati, Ohio, USA

    F. Bernard Daniel

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© 1990 Plenum Press, New York

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Hemminki, K., Randerath, K., Reddy, M.V. (1990). Postlabeling Analysis of Polycyclic Aromatic Hydrocarbon: DNA Adducts in White Blood Cells of Foundry Workers. In: Waters, M.D., Daniel, F.B., Lewtas, J., Moore, M.M., Nesnow, S. (eds) Genetic Toxicology of Complex Mixtures. Environmental Science Research, vol 39. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5850-3_23

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  • DOI: https://doi.org/10.1007/978-1-4684-5850-3_23

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5852-7

  • Online ISBN: 978-1-4684-5850-3

  • eBook Packages: Springer Book Archive

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