Summary
A lower level of cystatin C in cerebrospinal fluid (CSF) is one of the useful diagnostic markers of hereditary cerebral hemorrhage with amyloidosis in Iceland. Therefore we set up an assay to determine the level of cystatin C in CSF for diagnosis of cerebral amyloid angiopathy (CAA) associated with cerebral hemorrhage and cystatin C. We carried out an sandwich enzyme immunosorbent assay using monoclonal mouse anti-cystatin C and polyclonal rabbit anti-cystatin C antibodies. We examined CSF from nine cases of cerebral hemorrhage and fifty reference cases of other neurological diseases. Four patients with cerebral hemorrhage showed a low level of cystatin C and clinical manifestations suggestive of CAA. Our study showed that ELISA is useful for the diagnosis of CAA associated with cerebral hemorrhage and cystatin C.
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© 1990 Plenum Press, New York
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Shimode, K., Fujihara, S., Nakamura, M., Kobayashi, S., Tsunematsu, T. (1990). Diagnosis of Cerebral Amyloid Angiopathy with Cerebral Hemorrhage by Enzyme-Linked Immunosorbent Assay (ELISA) of Cystatin C in Cerebrospinal Fluid. In: Nagatsu, T., Fisher, A., Yoshida, M. (eds) Basic, Clinical, and Therapeutic Aspects of Alzheimer’s and Parkinson’s Diseases. Advances in Behavioral Biology, vol 38A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5844-2_22
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DOI: https://doi.org/10.1007/978-1-4684-5844-2_22
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5846-6
Online ISBN: 978-1-4684-5844-2
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