Abstract
Eight monoclonal antibodies (MAbs) were raised against a bovine Coronavirus (B.C.V.) which had been isolated in Scotland and was designated S2. The MAbs were divided into two groups on the basis of their reactions with S2 virus in indirect immunofluorescence (I.F.), neutralisation and haemagglutination inhibition (H.A.I.) tests. Five of the MAbs were positive by all three tests but failed to bind to proteins in Western immunoblotting experiments. The remaining three MAbs were positive in I.F. tests only, two of which were shown to bind to the 52K nucleocapsid protein by Western immunoblotting. Different patterns of antigen distribution within infected cells were demonstrated when the MAbs were used in the I.F. test. However only minor strain variations were detected by I.F. and H.A.I. tests when the MAbs were tested against each of five cell culture adapated strains of B.C.V. Twenty-nine isolates of B.C.V. have been grown in neonatal calf tracheal organ cultures: attempts are being made to further characterise these isolates.
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
B. King and D.A. Brian. Bovine Coronavirus structural proteins. J. Virol. 42:700 (1982).
D. Deregt, M. Sahara, and L.A. Babiuk. Structural proteins of bovine Coronavirus and their intracellular processing. J. gen. Virol. 68: 2863 (1987).
D. Deregt and L.A. Babiuk. Monoclonal antibodies to bovine Coronavirus: Characteristics and topographical mapping of neutralising epitopes on the E2 and E3 glycoproteins. Virology, 161: 410 (1987).
D. Deregt, G.A. Gifford, M.K. Ijaz, T.C. Watts, J.E. Gilchrist, D.M. Haines, and L.A. Babiuk. Monoclonal antibodies to bovine Coronavirus glycoproteins E2 and E3: Demonstration of in vivo virus -neutralizing activity. J. gen. Virol. 70: 993 (1989).
B. King, B.J. Potts, and D.A. Brian. Bovine Coronavirus haemagglutinin protein. Virus Res. 2: 53 (1985).
M.D. Parker, G.J. Cox, D. Deregt, D.R. Fitzpatrick, and L.A. Babiuk. Cloning and in vitro expression of the gene for the E3 haemagglutinin glycoprotein of bovine Coronavirus. J. gen. Virol. 70: 155 (1989).
A.A. El-Ghorr, D.R. Snodgrass, P.M.M. Scott, and I. Campbell. A serological comparison of bovine Coronavirus strains. Arch. Virol. 104: 241 (1989).
J.F. Vautherot, J. Laporte, M.F. Madelaine, P.Bobulesco, and A. Roseto. Antigenic and polypeptide structure of bovine enteric Coronavirus as defined by monoclonal antibodies. Adv. Exp. Med. Biol. 173: 117 (1984).
S. Dea, R.S. Roy, and M.A.S.Y. Elazhary. Antigenic variations among calf diarrhoea coronaviruses by immunodiffusion and counterimmunoelectrophoresis. Ann. Rech. Vet. 13(4): 351 (1982).
E.J. Stott, L.H. Thomas, J.C.Bridger, and N.J. Jebbett. Replication of a bovine Coronavirus in organ cultures of foetal trachea. Vet. Microbiol. 1: 65 (1976).
J.C. Bridger, G.N. Woode, and A. Meyling. Isolation of coronaviruses from neonatal calf diarrhoea in Great Britain and Denmark. Vet. Microbiol. 3:101 (1978).
M.S. McNulty, D.G. Bryson, G.M. Allan, and E.F. Logan. Coronavirus infection of the bovine respiratory tract.VetMicrobiol. 9:425 (1984).
A.A. El-Ghorr, D.R. Snodgrass, and F.M.M. Scott. Evaluation of an immunogold electron microscopy technique for detecting bovine Coronavirus. J. Virol. Methods. 19:215 (1988).
C.A. Mebus, E.L. Stair, M.B. Rhodes, and M.J. Twiehaus. Neonatal calf diarrhoea: Propagation, attenuation, and characteristics of a coronavirus-like agent. Am. J. Vet. Res. 34:145 (1973).
S. Dea, R.S. Roy, and M.E. Begin. Physicochemical and biological properties of neonatal calf diarrhoea coronaviruses isolated in Quebec and comparison with the Nebraska calf Coronavirus. Am. J. Vet. Res. 41:23 (1980).
D.J. Reynolds, T.G. Debney, G.A. Hall, L.H. Thomas, and K.R. Parsons. Studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves. Arch. Virol. 85: 71 (1985).
L.H. Thomas and C.J. Howard. Effect of mycoplasma dispar, mycoplasma bovirhinis, acholeplasma laidlawii and T-mycoplasmas on explant cultures of bovine trachea. J. Comp. Path. 84: 193 (1974).
A.R. Collins, R.L. Knobler, H. Powell, and M.J. Buchmeier. Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. Virology, 119:358 (1982).
H. Laude, J-M. Chapsal, J. Gelfi, S. Labiau, and J. Grosclaude. Antigenic structure of transmissible gastroenteritis virus. I. Properties of monoclonal antibodies directed against virion proteins. J. gen. Virol. 67: 119 (1986).
S. Dea and P. Tijssen. Antigenic and polypeptide structure of turkey enteric coronaviruses as defined by monoclonal antibodies. J. gen. Virol. 70: 1725 (1989).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Plenum Press, New York
About this paper
Cite this paper
Clark, M.A., Campbell, I., El-Ghorr, A.A., Snodgrass, D.R., Scott, F.M.M. (1990). A Comparison of Bovine Coronavirus Strains Using Monoclonal Antibodies. In: Cavanagh, D., Brown, T.D.K. (eds) Coronaviruses and their Diseases. Advances in Experimental Medicine and Biology, vol 276. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5823-7_64
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5823-7_64
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5825-1
Online ISBN: 978-1-4684-5823-7
eBook Packages: Springer Book Archive