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Transduction in Biological Systems pp 401–414Cite as

A Pharmacological Approach to the Physiological Mechanism of Excitation-Contraction Coupling

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Abstract

The use of pharmacologic agents as tools to aid in the characterization and separation of physiological processes is not a new idea. The availability of specific toxins like tetrodotoxin and saxitoxin made it easy to test for the presence of sodium channels in excitable cells and made possible the purification(1) reconstitution(2,3) and cloning(4) of such channels. We hope to utilize a pharmacologic approach here to determine whether a Ca2+ -channel isolated recently from skeletal muscle sarcoplasmic reticulum (SR) is the one of physiological importance. Is it the right channel? We know several methods exist to cause Ca2+ release from SR.(5,6) If we had specific inhibitors for each form of release, we could test whether the releases or channels they blocked were involved in excitation-contraction coupling. We will examine the role of Ca2+ -induced Ca2+ release channels first because these are the channels already isolated.

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Author information

Authors and Affiliations

  1. Department of Physiology and Biophysics, The University of Texas Medical Branch, Galveston, Texas, 77550, USA

    Philip Palade, Donald Brunder, Christine Dettbarn & Philip Stein

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  1. Philip Palade
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  2. Donald Brunder
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  3. Christine Dettbarn
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  4. Philip Stein
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Editor information

Editors and Affiliations

  1. Centro de Estudios Científicos de Santiago, Universidad de Chile, Santiago, Chile

    Cecilia Hidalgo  & Enrique Jaimovich  & 

  2. Universidad de Chile, Santiago, Chile

    Juan Bacigalupo

  3. University of California at Los Angeles, Los Angeles, California, USA

    Julio Vergara

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© 1990 Plenum Press, New York

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Palade, P., Brunder, D., Dettbarn, C., Stein, P. (1990). A Pharmacological Approach to the Physiological Mechanism of Excitation-Contraction Coupling. In: Hidalgo, C., Bacigalupo, J., Jaimovich, E., Vergara, J. (eds) Transduction in Biological Systems. Series of the Centro de Estudios Científicos de Santiago. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5736-0_27

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  • DOI: https://doi.org/10.1007/978-1-4684-5736-0_27

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5738-4

  • Online ISBN: 978-1-4684-5736-0

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