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Calbindin-D28 in Mammalian Brain, Retina, and Endocrine Pancreas: Immunohistochemical Comparison with Calretinin

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Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 255)

Abstract

Calbindin 28K and calretinin are very similar calcium binding proteins which are both present in the central nervous system (CNS). They respectively bind 4 and 5 Ca++ ions. We have compared by immunohistochemistry and in situ hybridization their localisation in the brain and the retina. The two proteins are generally expressed in different neurons with a few neurons containing both calcium binding proteins. Calbindin 28K is also present in the endocrine system. We have examined the cellular distribution of calbindin in the pancreatic endocrine cells of chick, rat and human and found variable distribution among the different endocrine cell types. We also describe the presence of calbindin in RINm5F cells, an insulin-producing tumor cell line derived from a radiationinduced rat insulinoma.

Keywords

  • Endocrine Pancreas
  • Islet Cell Tumor
  • RINm5F Cell
  • Endocrine Cell Type
  • Vanadyl Sulfate

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  • Baimbridge KG, Miller JJ and Parkes CO (1982). Calcium-binding protein distribution in the rat brain. Brain Res 239: 519–525.

    PubMed  CrossRef  CAS  Google Scholar 

  • Gallifret Y (1968). Les diverses aires fonctionnelles de la rétine du Pigeon. Zeitschrift für Zellforschung. 86: 535–545.

    CrossRef  Google Scholar 

  • Garcia-Segura LM, Baetens D, Roth J, Norman AW, Orci L (1984). Immunohistochemical mapping of calcium-binding protein immunoreactivity in the rat central nervous system. Brain Res 296: 75–86.

    PubMed  CrossRef  CAS  Google Scholar 

  • Gazdar AF, Chick WL, Oie HK, Sims HL, King DL, Weir GC and Lauris V (1980). Continuous, clonal, insulin-and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. Proc Natl Acad Sci, USA, 77: 3519–3523.

    PubMed  CrossRef  CAS  Google Scholar 

  • Giroix MH, Malaisse-Lagae F, Sener A and Malaisse WJ (1985). Hescose metabolism in pancreatic islet. Galactose transport, Phosphorylation and oxidation. Mol Cell Biochem, 66: 61–64.

    PubMed  CrossRef  CAS  Google Scholar 

  • Jande SS, Maler L and Lawson DEM (1981). Immunohistochemical mapping of vitamin D-dependent calcium-binding protein in brain. Nature (London) 294: 765–767.

    CrossRef  CAS  Google Scholar 

  • Langeron M (1949). Précis de microscopic 7e edition, Masson et Cie, Editeurs, Paris.

    Google Scholar 

  • Norman AW, Roth J and Orci L (1982). The vitamin D endocrine system: steroid metabolism, hormone receptors and biological response (CaBPs). Endocrinol Rev, 3: 331–366.

    CrossRef  CAS  Google Scholar 

  • Parmentier M, Lawson DEM, and Vassart G (1987) Human calbindin complementary DNA sequence. Evolutionary and functional implications. Eur J Biochem, 170: 207–215.

    PubMed  CrossRef  CAS  Google Scholar 

  • Parmentier M, Ghysens M, Rypens F, Lawson DEM, Pasteeis JL, Pochet R (1987b). Calbindin in vertebrate classes. Immunohistochemical localization and Western blot analysis. Gen Comp Endocrinol. 65: 399–407.

    PubMed  CrossRef  CAS  Google Scholar 

  • Parmentier M, Pohl V and Vassart G (1988). A calbindin 29 KDA: a new neuronal protien. Abstract p. 181, Sixth International Symposium on Calcium-binding proteins in Health and Disease, Nagoya, Japan, July 1988.

    Google Scholar 

  • Pasteeis B, Parmentier M, Lawson DEM, Verstappen A and Pochet R (1987). Calcium binding protein immunoreactivity in pigeon retina. Investigative Ophthal. Visual Sci., 28: 658–664.

    Google Scholar 

  • Pochet R, Parmentier M, Lawson DEM, Pasteeis JL (1985). Rat brain synthesizes two vitamin D-dependent calcium-binding proteins. Brain Res 345: 251–256.

    PubMed  CrossRef  CAS  Google Scholar 

  • Pochet R, Pipeleers DG and Malaisse WJ (1987). Calbindin D 27 kDa: preferential localization in non-B islet cells of the rat pancreas. Biol. of the Cell., 61: 155–161.

    CrossRef  CAS  Google Scholar 

  • Résibois A, Rypens F and Pochet R (1988). Epithelial and neuronal calbindin in avian intestine: an immunohistochemical study. Cell and Tissue Res 251: 611–620.

    CrossRef  Google Scholar 

  • Rogers JH (1987). Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons. The J Cell Biol 105: 1343–1353.

    CrossRef  CAS  Google Scholar 

  • Roman A, Brisson P, Pasteels B, Demol S, Pochet R and Collin JP (1988). Pinealretinal molecular relationship; immunocytochemical evidence of calbindin 27-kDa in pineal transducers. Brain Res., 442: 33–42.

    PubMed  CrossRef  CAS  Google Scholar 

  • Spencer R, Charman M, Wilson PW, Lawson DEM (1978). The relationship between vitamin D stimulated calcium transport and intestinal CaBP in the chicken. Biochem J 170:93–101.

    PubMed  CAS  Google Scholar 

  • Wilson PW, Harding M, Lawson DEM (1985). Putative amino acid sequence of chick calcium-binding protein deduced from a complementary DNA sequence. Nucleic Acid Res. 13: 8867–8881.

    PubMed  CrossRef  CAS  Google Scholar 

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© 1989 Plenum Press, New York

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Pochet, R. et al. (1989). Calbindin-D28 in Mammalian Brain, Retina, and Endocrine Pancreas: Immunohistochemical Comparison with Calretinin. In: Hidaka, H., Carafoli, E., Means, A.R., Tanaka, T. (eds) Calcium Protein Signaling. Advances in Experimental Medicine and Biology, vol 255. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5679-0_46

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  • DOI: https://doi.org/10.1007/978-1-4684-5679-0_46

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5681-3

  • Online ISBN: 978-1-4684-5679-0

  • eBook Packages: Springer Book Archive