Demonstration of Different Regional Distributions of Calcineurin Subunits using Monoclonal Antibodies

  • Hideki Matsui
  • Toshifumi Itano
  • Seiji Etoh
  • Masaaki Tokuda
  • Jerry H. Wang
  • Osamu Hatase
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 255)


Immunohistochemical localizations of calcineurin subunits A (60KDa) and B (20KDa) were examined in a rat brain using subunit specific monoclonal antibodies. The immunoreactivity of the subunit A was abundant in the hippocampus, in the striatum, and in the thalamus, but weak in the neocortex. On the contrary, the immunoreactivity of the subunit B was more abundant in the cortex, and it was more ubiquitous than subunit A.

The distribution of subunit A in the rat brain very well agreed with that of zinc, which is an intrinsic metal ion and a potent inhibitor of calcineurin phosphatase.


Pyramidal Cell Mossy Fiber Immunohistochemical Localization Apical Dendrite Stratum Radiatum 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Hideki Matsui
    • 1
  • Toshifumi Itano
    • 1
  • Seiji Etoh
    • 1
  • Masaaki Tokuda
    • 1
  • Jerry H. Wang
    • 2
  • Osamu Hatase
    • 1
  1. 1.Dept. of PhysiologyKagawa Med. Sch.KagawaJapan
  2. 2.Dept. of Medical BiochemistryUniv. of CalgaryCalgaryCanada

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