Abstract
In relaxed smooth muscle the contractile apparatus is dormant and the initiation of contraction requires an activation. It is generally agreed that the activation is achieved by phosphorylation of the two 20,000-dalton light chains of myosin (LC20) and usually Serine 19 is phosphorylated. This process is catalyzed by myosin light chain kinase (MLCK) and the Ca2+-dependence of the system is due to the activation of the MLCK apoenzyme by the Ca2+4 calmodulin (CaM) complex. Dephosphorylation and inactivation of the contractile apparatus, reflects the activity of a light chain phosphatase. The latter, however, has not been well characterized and it is not known, for example, if one or more phosphatases are involved or, if the phosphatase activity is regulated.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
D. J. Hartshorne, Biochemistry of the contractile process in smooth muscle, in “Physiology of the Gastrointestinal Tract” 2nd. Ed. L. R. Johnson, ed., Raven Press, N.Y. pp. 423–482 (1987).
P. F. Dillon, M. O. Aksoy, S. P. Driska, and R. A. Murphy, Myosin phosphorylation and the cross bridge cycle in arterial smooth muscle, Science. 211:495–497 (1981).
M. O. Aksoy, S. Mras, K. E. Kamm, and R. A. Murphy, Ca++, cAMP, and changes in myosin phosphorylation during contraction of smooth muscle, Am. J. Physiol.. 245: C255–C270 (1983).
P. J. Silver, and J. T. Stull, Regulation of myosin light chain and Phosphorylase phosphorylation in tracheal smooth muscle, J. Biol. Chem., 257:6145–6150 (1982).
P. J. Silver, and J. T. Stull, Phosphorylation of myosin light chain and Phosphorylase in tracheal smooth muscle in response to KCl and carbachol, Mol. Pharmacol.. 25:267–274 (1984).
R. A. Murphy, Myosin phosphorylation and cross bridge regulation in arterial smooth muscle, State-of-the-art review, Hypertension, 4(Suppl. 2):3–7 (1982).
C-M. Hai, and R. A. Murphy, Cross bridge phosphorylation and regulation of latch state in smooth muscle, Am. J. Physiol. 254:C99–C106 (1988).
S. P. Driska, High myosin light chain phosphatase activity in arterial smooth muscle: can it explain the latch phenomenon? in “Smooth Muscle Contraction”, M. J. Siegman, ed., A. R. Liss, Inc., N.Y. 387–398 (1987).
H. Suzuki, H. Onishi, K. Takahashi, and S. Watanabe, Structure and function of chicken gizzard myosin, J. Biochem (Tokyo), 84:1529–1542 (1978).
M. Ikebe, S. Hinkins, and D. J. Hartshorne, Correlation of enzymatic properties and conformation of smooth muscle myosin, Biochemistry. 22:4580–4587 (1983).
D. J. Hartshorne, R. F. Siemankowski, and O. M. Aksoy, Ca regulation in smooth muscle and phosphorylation: Some properties of the myosin light chain kinase. In: Regulatory Mechanism of Muscle Contraction, S. Ebashi, K. Maruyama, and M. Endo, ed., Japan Society for the Promotion of Science and Fujihara Foundation of Science, Tokyo, 287–301, (1980).
M. Ikebe, R. J. Barsotti, S. Hinkins, and D. J. Hartshorne, Effects of magnesium chloride on smooth muscle actomyosin adenosine-5′-triphosphatase activity, myosin conformation and tension development in glycerinated smooth muscle fibers, Biochemistry 23:5062–5068 (1984).
E. M. V. Pires, and S. V. Perry, Purification and properties of myosin light-chain kinase from fast skeletal muscle, Biochem. J., 167:137–146 (1977).
T. S. Chandra, N. Nath, H. Suzuki, and J. C. Seidel, Modification of thiols of gizzard myosin alters ATPase activity, stability of myosin filaments, and the 6–10S conformational transition, J. Biol. Chem. 260:202–207 (1985).
S. Srivastava, M. Ikebe, and D. J. Hartshorne, Trinitrophenylation of smooth muscle myosin, Biochem. Biophys. Res. Commun.. 126:748–755 (1985).
H. Onishi, and S. Watanabe, Correlation between the papain digestibility and the conformation of 10S-myosin from chicken gizzard, J. Biochem. (Tokyo), 95:899–902 (1984).
M. Ikebe, and D. J. Hartshorne, Conformation-dependent proteolysis of smooth-muscle myosin, J. Biol. Chem. 259:11639–11642 (1984).
M. Ikebe, and D. J. Hartshorne, Proteolysis of smooth muscle myosin by Staphylococcus aureus protease: Preparation of heavy meromyosin and subfragment 1 with intact 20,000-dalton light chains, Biochemistry. 24:2380–2386 (1985).
M. Ikebe, and D. J. Hartshorne, Proteolysis and actin-binding properties of 10S and 6S smooth muscle myosin, Identification of a site protected from proteolysis in the 10S conformation and by the binding of actin, Biochemistry. 25:6177–6185 (1986).
T. Marianne-Pepin, D. Mornet, R. Bertrand, J-P. Labbe, and R. Kassab, Interaction of the heavy chain of gizzard myosin heads with skeletal F-actin, Biochemistry. 24:3024–3029 (1985).
T. Katoh, and F. Morita, Interaction between myosin and F-actin. Correlation with actin-binding sites on subfragment-1, J. Biochem. (Tokyo), 96: 1223–1230 (1984).
A. Muhlrad, and M. F. Morales, Isolation and partial renaturation of proteolytic fragments of the myosin head, Proc. Natl. Acad. Sci. USA, 81:1003–1007 (1984).
D. Mornet, R. Bertrand, P. Pantel, E. Audemard, and R. Kassab, Structure of the actin-myosin interface, Nature 292:301–306 (1981).
M. Ikebe, Y. Tonomura, H. Onishi, and S. Watanabe, Elementary steps in the F-actin activated Mg2+-ATPase reaction of gizzard H-mero myosin:effects of phosphorylation of the light-chain subunit. J. Biochem. (Tokyo), 90:61–77 (1981).
H. Suzuki, W. F. Stafford III, H. S. Slayter, and J. C. Seidel, A conformational transition in gizzard heavy meromyosin involving the head-tail junction resulting in changes in sedimentation coefficient, ATPase activity, and orientation of heads, J. Biol. Chem., 260:14810–14817 (1985).
M. Ikebe, S. Hinkins, and D. J. Hartshorne, Correlation of intrinsic fluorescence and conformation of smooth muscle myosin, J. Biol. Chem., 258:14770–14773 (1983).
M. Higashihara, and M. Ikebe, Inhibition of 10S-6S conformational change of smooth muscle myosin by a monoclonal antibody, Biophys. J., 53:578a (1988).
A. V. Somlyo, T. M. Butler, M. Bond, and A. P. Somlyo, Myosin filaments have non-phosphorylated light chains in relaxed smooth muscle, Nature. 294:567–569 (1981).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Hartshorne, D.J., Ito, M., Ikebe, M. (1989). Myosin and Contractile Activity in Smooth Muscle. In: Hidaka, H., Carafoli, E., Means, A.R., Tanaka, T. (eds) Calcium Protein Signaling. Advances in Experimental Medicine and Biology, vol 255. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5679-0_30
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5679-0_30
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5681-3
Online ISBN: 978-1-4684-5679-0
eBook Packages: Springer Book Archive