Gene Expression of a Rat Cerebellar Ca-Binding Protein, Spot 35 Protein
About ten years ago we discovered a cerebellar protein, spot 35 protein, during the systematic analysis of the rat brain protein by using two dimensional gel-electrophoresis.1 Then we found that this spot 35 protein is an acidic Ca-binding protein by using 45Ca-binding assay procedure and is localized in Purkinje cells, their dendrite and axons using immunohistochemical examination.2,3 Translational experiment of cerebellar mRNA suggested the transcriptional regulation of gene expression of spot 35 protein.3 Next immunohistochemical studies revealed the developmental changes of Purkinje cells in the rat and human cerebella.4 Furthermore, our neurophysiological study showed involvement of spot 35 protein in modulation of Purkinje cell activity of rat cerebellum.5 Recently, we succeeded in cloning the cDNA encoding this protein.6,7 This cDNA was used to analyze the developmental and regional changes of mRNA coding for spot 35 protein in the rat brain, to probe the in situ hybridization of this mRNA in the rat cerebellum and to isolate the spot 35 gene in order to analyze its structure.
KeywordsPurkinje Cell Noncoding Region Human Cerebellum Internal Granule Cell Layer Purkinje Cell Activity
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