Abstract
We have investigated the suitability of applying primer directed dideoxynucleotide mediated sequencing methods to the characterization of low abundance messages. Direct mRNA sequence analysis is based on the priming of a target within a heterogeneous population of RNA by using oligonucleotide primers complimentary to the mRNA of interest. We have examined the minimal message abundance limits and mRNA amounts required for direct dideoxynucleotide RNA sequencing as a means of exploring the utility of this approach in characterizing point mutations.
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© 1989 Plenum Press, New York
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Joshi, R., Snyder, F.F. (1989). Sequencing of Mammalian Messenger RNA Using Oligonucleotide Primers: Application to Hypoxanthine Phosphoribosyltransferase and a Phosphoglycerate Kinase Variant. In: Mikanagi, K., Nishioka, K., Kelley, W.N. (eds) Purine and Pyrimidine Metabolism in Man VI. Advances in Experimental Medicine and Biology, vol 253A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5673-8_78
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DOI: https://doi.org/10.1007/978-1-4684-5673-8_78
Publisher Name: Springer, Boston, MA
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