ITP-Pyrophosphohydrolase and Purine Metabolism in Human Erythrocytes
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In a study on liquid erythrocyte preservation (de Verdier et al, 1987), we measured purine nucleotides in erythrocytes from 103 blood donors. We found ITP concentration above detection limit (0.05 mmol/1 in this study) in fresh erythrocytes from one donor and in erythrocytes stored for 42 days in CPD-SAGMAN solution (Högman et al, 1983) in another donor. The present study was designed in order to explore whether these erythrocytes showed any other sign of aberrant purine metabolism before or after storage. In view of an ongoing study on the use of the commonly prescribed purine analogue allopurinol in liver function evaluation (van Waeg et al, 1988a), the metabolism of allopurinol in normal and ITP-pyrophosphohydrolase (ITPase) deficient erythrocytes was also studied.
KeywordsHuman Erythrocyte Purine Base Purine Nucleoside Phosphorylase Salvage Rate Homozygote Mutant
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- Capuozzo E, Gigante M, Salerno C, Crifo C, 1986. Hypoxanthine transport through human erythrocyte membranes. Adv Exp Med Biol, 195B: 71–74.Google Scholar
- Hershko A, Razin A, Shoshani T, Mager J, 1967. Turnover of purine nucleotides in rabbit erythrocytes. II: Studies in vitro. Biochim Biophys Acta, 149: 59–73.Google Scholar
- Sweetman L, 1968. Urinary and cerebrospinal fluid oxypurine levels and allopurinol metabolism in the Lesch-Nyhan syndrome. Fedn Proc, 27: 1055–1059.Google Scholar