Abstract
Neoplastic cultured cells and tissues often exhibit altered plasma membrane morphologies and biochemical activities when compared to non-transformed counterparts [1]. However, accurate interpretation and reliability of data collected for such comparisons may be influenced greatly by the purity and composition of the plasma membrane fractions utilised. Non-uniform homogenisation, contamination by organelles other than plasma membrane and changes in culture conditions, together with the transformation process itself, all tend to confound the fractionation of plasma membranes from cells and hepatomas.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
D. Wallach, Membrane Molecular Biology of Neoplastic Cells, Elsevier, New York (1975)
S. Kashiwampta, S. Goto, R. Samba and F. Suzuki, Inhibition by bilirubin of (Na+-K+)-activated adenosine triphosphatase and K+-activated p-nitrophenyl phosphatase activities of nal-treated microsomes from young rat cerebrum J. Biol. Chem. 254:4577 (1979)
E.M. Croze and D.J. Morré, Isolation of plasma membrane, Golgi apparatus, and endoplasmic reticulum fractions from single homogenates of mouse liver, J. Cell Physiol 119:46 (1984)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Nowack, D.D., Morré, D.M., Morré, D.J. (1989). Plasma Membranes from Hepatomas and Cultured Cells by Aqueous Two-Phase Partition. In: Fisher, D., Sutherland, I.A. (eds) Separations Using Aqueous Phase Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5667-7_18
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5667-7_18
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5669-1
Online ISBN: 978-1-4684-5667-7
eBook Packages: Springer Book Archive