Abstract
The relative transparency of tissues to near infrared light means that it is possible to transilluminate intact organs. In the infrared region, oxygen dependent absorptions due to haemoglobin and cytochrome aa3 can be observed, and it is therefore possible to monitor changes in both the blood and tissue oxygenation of the organ.1 This monitoring technique is particularly applicable to the study of the brain since there is no interfering absorption from myoglobin, and recent technical developments of the instrumentation have made it possible to transilluminate 8–9 cm of brain tissue.2 However, once measurements of absorption change at several wavelengths are available, there are still considerable problems in converting this data into quantitative changes in the concentration of oxy and deoxy haemoglobin and of oxidised cytochrome aa3.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Jobsis F.F. “Non invasive infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters” Science, 198, 1264–1267 (1977).
Cope, M., Delpy, D.T. “System for long term measurement of cerebral blood and tissue oxygenation on newborn infants by near infrared transillumination” Med. & Biol. Eng. & Comp. 222, 183–190, (1988).
Cope, M., Delpy, D.T., Reynolds, E.O.R., Wray, S., Wyatt, J.S., van der Zee, P. “Methods of quantitating cerebral near infrared spectroscopy data” Adv. Exp. Med & Biol. 222, 183–190, (1988).
Wray, S., Cope, M., Delpy, D.T., Wyatt, J.S., Reynolds, E.O.R. “Characterisation of the near infrared absorption spectra of cytochrome aa3 and haemoglobin for the non invasive monitoring of cerebral oxygenation” Biochim, Biophys. Acta. 933, 184–192, (1988).
Delpy, D.T., Cope, M., van der Zee, P., Arridge, S., Wray S., Wyatt, J.S. “Estimation of optical path length through tissue from direct time of flight measurement” Phys. in Med. & Biol, (in press).
van der Zee, P., Delpy D.T. “Computed point spread functions for light in tissue using a measured volume scattering function.” Adv. Exp. Med. & Biol. 222, 191–198, (1988).
Svaasand, L.O., Ellinsen, R., “Optical properties of human brain” Photochem. & Photbiol. 38, 293–299, (1983).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Plenum Press, New York
About this chapter
Cite this chapter
Delpy, D.T. et al. (1989). Quantitation of Pathlength in Optical Spectroscopy. In: Rakusan, K., Biro, G.P., Goldstick, T.K., Turek, Z. (eds) Oxygen Transport to Tissue XI. Advances in Experimental Medicine and Biology, vol 248. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5643-1_5
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5643-1_5
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5645-5
Online ISBN: 978-1-4684-5643-1
eBook Packages: Springer Book Archive