Abstract
Kreuzer and Hoofd (1987) recently reviewed the experimental evidence, theoretical framework and physiological significance for facilitated O2 transport by hemoglobin in the red blood cell and by myoglobin in heart and red skeletal muscle. It is now well accepted that these vital biological proteins enhance tissue O2 delivery by carrier-mediated transport. However, many mechanistic details are not fully understood or their importance in vivo have not been completely evaluated. Much of the previous research during the past 30 years has been conducted with flat layers of carrier protein solutions subjected to known O2 concentration gradients in diffusion chambers. Facilitated O2 transport theory predicts how PO2 will vary with distance across the layer. In the present study, we measured PO2 profiles with recessed cathode microelectrodes (Whalen et al, 1967) across ca. 500 μm layers of aqueous solutions containing either hemoglobin or myoglobin. To our knowledge, there have been no previous attempts to evaluate facilitated O2 transport theory by actually measuring PO2 profiles in carrier protein solutions.
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References
Breepoel, P.M., de Koning, J., Hoofd, L., 1982; Diffusion of oxygen in methemoglobin solutions: dependence on salt concentrations, Biochem. Biophys. Res. Commun., 109:848–850.
Bouwer, S., 1987; Facilitated oxygen diffusion through hemoglobin solutions. Measurement of diffusion and reaction parameters, Ph.D. Thesis, Department of Physiology, Catholic University, Nijmegen, The Netherlands.
Buerk, D.G., Goldstick, T.K., 1982; Arterial wall oxygen consumption rate varies spatially, Am. J. Physiol., 243:H948–H958.
Buerk, D.G., 1985; An evaluation of Easton’s paradigm for the oxyhemoglobin dissociation curve, in: “Oxygen Transport to Tissue — VI,” Plenum Press, N.Y., Adv. Exp. Med. Biol., 180:333–344.
Buerk, D.G., Bridges, E.W., 1986; A simplified algorithm for computing the variation in oxyhemoglobin saturation with pH, PCO2, T and DPG, Chem. Eng. Commun., 47:113–124.
de Koning, J., Hoofd, LJ.C, Kreuzer, F., 1981; Oxygen transport and the function of myoglobin. Theoretical model and experiments in chicken gizzard smooth muscle, Pflügers Arch, 389:211–217.
Easton, D.M., 1979; Oxyhemoglobin dissociation curve as expo-exponential paradigm of asymmetric sigmoid function, J. Theor. Biol. 76:335–349.
Fletcher, J.E., 1980; On facilitated oxygen diffusion in muscle tissues, Biophys. J., 29:437–458.
Gayeski, T.E., Connett, R.J., Honig, C.R., 1987; Minimum intracellular PO2 for maximum cytochrome turnover in red muscle in situ, Am. J. Physiol., 252:H906–915.
Goldstick, T.K., Fatt, I., 1970; Diffusion of oxygen in solutions of blood proteins, Chem. Eng. Progr. Symp. Ser., 66:101–113.
Gonzalez-Fernandez, J.M., Atta, S.E., 1982; Facilitated transport of oxygen in the presence of membranes in the diffusion path, Biophys. J., 38:133–141.
Gutierrez, G., 1986; The rate of oxygen release and its effect on capillary O2 tension: a mathematical analysis, Resp. Physiol., 63:79–96.
Heliums, J.D., 1977; The resistance to oxygen transport in the capillaries relative to that in the surrounding tissue, Microvas. Res., 13:131–136.
Hoofd, L., Kreuzer, F., 1979; A new mathematical approach for solving carrier-facilitated diffusion problems, J. Math. Biol., 8:1–13.
Hoofd, L., Breepoel, P., Kreuzer, F., 1984; Facilitated diffusion and electrical potentials in protein solutions with ionic species, in: “Oxygen Transport to Tissue — V,” Plenum Press, N.Y., Adv. Exp. Med. Biol., 169:133–143.
Jacquez, J.A., 1984; The physiological role of myoglobin: More than a problem in reaction-diffusion kinetics, Math. Biosci., 68:57–97.
Kreuzer, F., Hoofd, L., 1987; Chapter 6, Facilitated diffusion of oxygen and carbon dioxide, in: “Handbook of Physiology, The Respiratory System, Vol. IV,” American Physiological Society, Bethesda, MD., pp. 89–111.
Kutchai, H., Jacquez, J.A., Mather, F.J., 1970; Nonequilibrium facilitated transport in hemoglobin solutions, Biophys. J., 10:38–54.
Mochizuki, M., Kagawa, T., 1986; Numerical solution of partial differential equations describing the simultaneous O2 and CO2 diffusions in the red blood cell, Jap. J. Physiol., 36:43–63.
Spaan, J.A.E., Kreuzer, F., van Wely, F.K., 1980; Diffusion coefficients of oxygen and hemoglobin as obtained simultaneously from photometric determination of the oxygenation of layers of hemoglobin solutions, Pflügers Arch, 384:241–251.
Whalen, W.J., Riley, J., Nair, P., 1967; A microelectrode for measuring intracellular PO2, J. Appl. Physiol., 23:798–801.
Whalen, W.J., 1971; Intracellular PO2 in heart and skeletal muscle, The Physiologist. 14:69–82.
Whalen, W.J., Buerk, D.G., Thuning, C.A., 1973a; Blood flow-limiting oxygen consumption in resting cat skeletal muscle, Am. J. Physiol. 224:763–768.
Whalen, W.J., Nair, P., Buerk, D., 1973b; Oxygen tension in the beating cat heart in situ, in: “Oxygen Supply — Theoretical and Practical Aspects of Oxygen Supply and Microcirculation of Tissue,” Urban and Schwarzenberg, West Germany, pp. 199–201.
Whalen, W.J., Nair, P., Buerk, D., Thuning, C.A., 1974; Tissue PO2 in normal and dener-vated cat skeletal muscle, Am. J. Phvsiol., 227:1221–1225.
Whalen, W.J., Buerk, D.G., Thuning, C.A., Kanoy, Jr., B.D., Duran, W.N., 1976; Tissue PO2, VO2, blood flow and perfusion pressure in resting dog gracilis muscle at constant flow, in: “Oxygen Transport to Tissue — II,” Plenum Press, N.Y., Adv. Exp. Med. Biol., 75:639–655.
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© 1989 Plenum Press, New York
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Buerk, D.G., Hoofd, L., Turek, Z. (1989). Microelectrode Studies of Facilitated O2 Transport Across Hemoglobin and Myoglobin Layers. In: Rakusan, K., Biro, G.P., Goldstick, T.K., Turek, Z. (eds) Oxygen Transport to Tissue XI. Advances in Experimental Medicine and Biology, vol 248. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5643-1_16
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DOI: https://doi.org/10.1007/978-1-4684-5643-1_16
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