Oxygen Distribution in Isolated Perfused Liver Observed by Phosphorescence Imaging
The measurement of oxygen in intact tissues has proven to be full of technical difficulties. For example, the direct determination of oxygen in tissue using microelectrodes is subject to error in the low range of oxygen pressures (< 1 uM) and is restricted to fixed sites of evaluation (Silver, 1984). Moreover, insertion of the electrode damages the tissue and disturbs the local milieu. Indirect qualitative measurements using surface fluorescence of NADH indicate the presence of anoxia in tissue (Barlow and Chance, 1976) but lacks internal standardization (Koretsky et al., 1987). Spectrophotometric measurements of hemoglobin (Kekonen et al, 1987) and myoglobin (Fabel and Lubbers, 1965; Tamura et al., 1978) are useful for oxygen pressures near their P50 but are subject to optical interference from changes in light scattering and from absorbtion by other chromophores. Alternatively, cryomicrospectrophotometry of myoglobin (Gayeski and Honig, 1986) can provide an accurate method for calculating intracellular oxygen pressure in some tissues. Because this method requires freezing of the tissue, it is impractical for monitoring changes in oxygenation during any length of time.
KeywordsPortal Vein Gray Level Oxygen Pressure Pyridine Nucleotide Oxygen Distribution
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