Toward Absolute Reflectance Oximetry: I. Theoretical Consideration for Noninvasive Tissue Reflectance Oximetry

  • Setsuo Takatani
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 248)


Methods of absorption and reflection photometry are sensitive enough to allow in vivo measurement of pigments such as hemoglobin, myoglobin and cytochromes which participate in oxygen transport to tissues. Since the influence of light on these pigments can be made really small, transmission and reflection photometry can be useful tools to noninvasively evaluate the oxygenation process that takes place in various tissues. Historically, the application of the spectrophotometry method for measurement of tissue oxygenation in situ has been made first in 1930-40’s by Kramer(1934), Matthes(1934), Millikan(1942) and Brinkman(1949) in which two wavelengths were employed, one for the measurement of pigment concentration and the other for compensation of nonspecific light loss by tissue. In the 1950’s, the two wavelength method was very much improved by Chance (1954).


Tissue Model Oxygen Difference Theoretical Reflectance Tissue Oxygen Saturation Tissue Hemoglobin 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Setsuo Takatani
    • 1
  1. 1.National Cardiovascular Center Research InstituteSuita, OsakaJapan

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