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Biologically Based Methods for Cancer Risk Assessment pp 193–207Cite as

Alterations in Gene Expression in Mouse Hepatocarcinogenesis

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Abstract

Alteration in gene expression is one of several molecular changes observed in cancer. The great relevance on cell phenotype of quantitative alterations in the levels of particular mRNAs is well documented. To isolate genes under-expressed in mouse liver tumors compared to normal adult liver, we have screened a normal adult liver cDNA library with RNA probes prepared from a normal adult liver and from a hepatocellular carcinoma. Three different clones showed the common feature to be expressed at relatively high levels in normal adult liver and to be undetectable or expressed at 10–50-fold lower levels, in liver carcinomas and in 14-day-old normal liver. Therefore, these clones should represent genes regulated during liver development. Preliminary nucleotide sequence analysis of a 3′ terminal region of one clone indicated that the clone may be identical to mouse major urinary protein. The characterization of the other two clones is in progress.

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Abbreviations

AFB1:

aflatoxin B1

CD:

choline-deficient diet

CDE:

choline-deficient diet containing ethionine

DAB:

pdimethylaminoazobenzene

DEN:

diethylnitrosoamine

HO-DHE:

l′-hydroxy-2′,3′-dehydroestragole

3′-Me-DAB:

3′-methyl-4-dimethylaminoazobenzene

MUP:

major urinary protein

N-HO-AAF:

N-hydroxy-2-acetylaminofluorene

PB:

Phenobarbital

TPA:

12-0-tetradecanoyl phorbol-13-acetate

PDGF:

platelet-derived growth factor

VC:

vinyl carbamate

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Authors and Affiliations

  1. Division of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy

    Tommaso A. Dragani, Giacomo Manenti, Marina R. M. Sacchi, Bruno Colombo & Giuseppe Della Porta

Authors
  1. Tommaso A. Dragani
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  2. Giacomo Manenti
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  3. Marina R. M. Sacchi
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  4. Bruno Colombo
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  5. Giuseppe Della Porta
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Editor information

Editors and Affiliations

  1. Office of Risk Analysis, Oak Ridge National Laboratory, 37831-6109, Oak Ridge, Tennessee, USA

    Curtis C. Travis

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© 1989 Plenum Press, New York

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Dragani, T.A., Manenti, G., Sacchi, M.R.M., Colombo, B., Porta, G.D. (1989). Alterations in Gene Expression in Mouse Hepatocarcinogenesis. In: Travis, C.C. (eds) Biologically Based Methods for Cancer Risk Assessment. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5625-7_15

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  • DOI: https://doi.org/10.1007/978-1-4684-5625-7_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5627-1

  • Online ISBN: 978-1-4684-5625-7

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