Abstract
Subconfluent cultures of 3T3 fibroblasts were labeled to isotopic equilibrium with [3 H]glycerol and analyzed by thin-layer chromatography after extraction of lipids to determine the levels of [3 H]diacylglycerol (DAG) in comparison to the total amount of3 H incorporated into cellular lipid. The transformed cells used in this study were always compared to their true parental clone and were of recent origin to decrease the possibility that observed changes are merely reflecting long-term cell divergence.
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Blackshear, P. J., Wen, L., Glynn, B. P., and Witters, L. A., 1986. Protein kinase C-stimulated phosphorylation in vitro of an Mr 80,000 protein phosphorylated in response to phorbol esters and growth factors in intact fibroblasts, J. Biol. Chem. 261:1459–1469.
Faletto, D. L., Arrow, A. S., and Macara, I. G., 1985, An early decrease in phosphatidylinositol turnover occurs on induction of Friend cell differentiation and precedes the decrease in c-myc expression, Cell 43:315–325.
Farese, R. V., Konda, T. S., Davis, J. S., Standaert, M. L., Pollet, R. J., and Cooper, D. R., 1987, Insulin rapidly increases diacylglycerol by activating de novo phosphatidic acid synthesis, Science 236:586–589.
Kamata, T., Sullivan, N. F., and Wooten, M. W., 1987, Reduced protein kinase C activity in a ras - resistant cell line derived from KiMSV transformed cells, Oncogene 1:37–46.
Lacal, J., Moscat, J., and Aaronson, S. A., 1987, Novel Source of 1,2-diacylglycerol elevated in cells, transformed by the Ha-røs oncogene, Nature 330:269–271.
Macara, I. G., 1985, Oncogenes, ions and phospholipids, Am. J. Physiol. 248:C3–C11.
Macara, I. G., 1989, Elevated phosphocholine in røs-transformed 3T3 cells from increased choline kinase activity, not from phosphatidylcholine breakdown, Mol. Cell. Biol. (in press).
Moolenar, W. H., Yarden, Y., deLaat, S. W., and Schlessinger, J., 1982, Epidermal growth factor induces electrically silent Na+ influx in human fibroblasts, J. Biol. Chem. 257:8502–8506.
Rozengurt, E., 1981, Stimulation of Na+ influx, Na-K pump activity and DNA synthesis in quiescent cultured cells, Adv. Enz. Regul. 19:61–85.
Rozengurt, E., Rodriquez-Pena, M., and Smith, K. A., 1983, Phorbol esters, phospholipase C and growth factors rapidly stimulate the phosphorylation of a Mr 80,000 protein in intact quiescent 3T3 cells, Proc. Natl. Acad. Sci. USA 80:7244–7248.
Wolfman, A., and Macara, I. G., 1987, Elevated levels of diacylglycerol and decreased phorbol ester sensitivity in ras-transformed fibroblasts, Nature 325:359–361.
Wolfman, A., Wingrove, T., Blackshear, P. J., and Macara, I. G., 1987, Down-regulation of protein kinase C and of an endogenous 80-kDa substrate in transformed fibroblasts, J. Biol. Chem. 262: 16546–16552.
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© 1989 Plenum Press, New York
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Macara, I.G. et al. (1989). Growth Factors, Oncogenes, and Protein Kinase C. In: Fiskum, G. (eds) Cell Calcium Metabolism. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5598-4_27
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DOI: https://doi.org/10.1007/978-1-4684-5598-4_27
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