Abstract
Phosphoinositide metabolism has been known to play a crucial role in signal transduction systems of various cells, producing two second messengers (Nishizuka 1984; Berridge and Irvine, 1984). One is 1,2-diacylglycerol (1,2-DG), which directly activates protein kinase C., and the other is inositol-l,4,5-trisphosphate, which releases Ca2+ from internal storage sites. Recently, several lines of evidence have been revealing the involvement of guanine nucleotide-binding regulatory protein (G protein) in agonist-induced activation of phospholipase C., analogous to the adenylate cyclase system (Cockcroft, 1987; Haslam and Davidson, 1984; Cockcroft and Gomperts, 1985; Bradford and Rubin, 1986; Brasset al., 1986; Kikuchiet al., 1986; Lapetina, 1986). Upon stimulation of various types of inflammatory cells, arachidonic acid is released and converted to physiologically active substances, prostaglandins and leukotrienes. Although the coupling of phospholipase C with G protein has been inten sively studied, only limited information is available regarding the involvement of G protein in phospholipase A2activation (Burchet al., 1986; Jelsema, 1987; Benjaminet al., 1987; Nakashimaet al., 1987). Possible involvement of G protein in phospholipase A2activation was investigated by examining the effects of guanine nucleotides (GTP or its nonhydrolyzable analog, GTPĪ³S) on generation of free arachidonic acid in permeabilized inflammatory cells, including saponin-permeabilized human platelets and ATP-permeabilized rat peritoneal mast cells.
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References
Bell, R. L., Kennedy, D. A., Stanford, N., and Majerus, P. W., 1979, Diglyceride lipase: A pathway for arachidonate release from human platelets, Proc. Natl. Acad. Sci. USA 76:3238ā3241.
Benjamin, C. W., Tarpley, W. G., and Gorman, R. R., 1987, Loss of platelet-derived growth factorstimulated phospholipase activity in NIH-3T3 cells expressing the EJ-ras oncogene, Proc. Natl. Acad. Sci. USA 84:546ā550.
Berridge, M. J., and Irvine, R. F., 1984, Inositol trisphosphate, a novel second messenger in cellular signal transduction, Nature 312:315ā321.
Besterman, J. M., Duronio, V., and Cuatrecasas, P., 1986, Rapid formation of diacylglycerol from phosphatidylcholine: A pathway for generation of a second messenger, Proc. Natl. Acad. Sci. USA 83:6785ā6789.
Bills, T. K., Smith, J. B., and Silver, M. J., 1977, Selective release of arachidonic acid from the phospholipids of human platelets in response to thrombin, J. Clin. Invest. 60:1ā6.
Bokoch, G. M., and Gilman, A. G., 1984, Inhibition of receptor-mediated release of arachidonic acid by pertussis toxin, Cell 39:301ā308.
Bradford, P. G., and Rubin, R. P., 1986, Guanine nucleotide regulation of phospholipase C activity in permeabilized rabbit neutrophils, Biochem . J. 239:97ā102.
Brass, L. F., Laposata, M., Banga, H. S., and Rittenhouse, S. E.,1986, Regulation of the phosphoi-nositide hydrolysis pathway in thrombin-stimulated platelets by a pertussis toxin-sensitive guanine nucleotide-binding protein: Evaluation of its contribution to platelet activation and comparisons with the adenylate cyclase inhibitory protein, Gi, J. Biol. Chem. 261:16838ā16847.
Burch, R. M., Luini, A., and Axelrod, J., 1986, Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to Ī±1-adrenergic stimulation in FRTL5 thyroid cells, Proc. Natl. Acad. Sci. USA 83:7201ā7205.
Cockcroft, S., 1987, Polyphosphoinositide phosphodiesterase: Regulation by a novel guanine nucleotide binding protein, Gp, Trends Biochem. Sci. 12:75ā78.
Cockcroft, S., and Gomperts, B. D., 1979, Activation and inhibition of calcium-dependent histamine secretion by ATP ions applied to rat mast cells, J. Physiol. (Lond.) 296:229ā243.
Cockcroft, S., and Gomperts, B. D., 1985, Role of nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase, Nature 314:534ā536.
Daniel, L. W., Waite, M., and Wykle, R. L., 1986, A novel mechanism of diglyceride formation: 12-0-Tetradecanoylphorbol-13-acetate stimulates the cyclic breakdown and resynthesis of phos-phatidylcholine, J. Biol. Chem. 261:9128ā9132.
Haslam, R. J., and Davidson, M. M. L., 1984, Receptor-induced diacylglycerol formation in permea-bilized platelets: Possible role for a GTP-binding protein, J. Receptor Res. 4:605ā629.
Jelsema, C. L., 1987, Light activation of phospholipase A2 in rod outer segments of bovine retina and its modulation by GTP-binding proteins, J. Biol. Chem. 262:163ā168.
Kikuchi, A., Kozawa, O., Kaibuchi, K., Katada, T., Ui, M., and Takai, Y., 1986, Direct evidence for involvement of a guanine nucleotide-binding protein in chemitactic peptide-stimulated formation of inositol bisphosphate and trisphosphate in differentiated human leukemic (HL-60) cells: Recon-stitution with Gi or Go of the plasma membrane ADP-ribosylated by pertussis toxin, J. Biol. Chem. 261:11558ā11562.
Lapetina, E. G., 1986, Effect of pertussis toxin on the phosphodiesteratic cleavage of the polyphos-phoinositides by guanosine 5ā²-O-thiotriphosphate and thrombin in permeabilized human platelets, Biochem . Biophys. Acta 884:219ā224.
Litosch, I., Wallis, C., and Fain, J. N., 1985, 5-Hydroxytryptamine stimulates inositol phosphate production in a cell-free system from blowfly salivary glands: Evidence for a role of GTP in coupling receptor activation to phosphoinositide breakdown, J. Biol. Chem. 260:5464ā5471.
Martin, T. W., Wysolmerski, R. B., and Lagunoff, D., 1987, Phosphatidylcholine metabolism in endothelial cells: evidence for phospholipase A and a novel Ca2+ -independent phospholipase C., Biochim . Biophys. Acta 917:296ā307.
Nakamura, T., and Ui, M., 1984a, Simultaneous inhibitions of inositol phospholipid breakdown, ara-chidonic acid release, and histamine secretion in mast cells by islet-activating protein, pertussis toxin, J. Biol. Chem. 260:3584ā3593.
Nakamura, T., and Ui, M., 1984b, Islet-activating protein, pertussis toxin, inhibits Ca2+ -induced and guanine nucleotide-dependent releases of histamine and arachidonic acid from rat mast cells, FEBS Lett . 173:414ā418.
Nakashima, S., Tohmatsu, T., Hattori, H., Suganuma, A., and Nozawa, Y., 1987, Guanine nucleotides stimulate arachidonic acid release by phospholipase A2 in saponin-permeabilized human platelets, J. Biochem. 101:1055ā1058.
Nishizuka, Y., 1984, The role of protein kinase C in cell surface signal transduction and tumour promotion, Nature 308:693ā698.
Okano, Y., Yamada, K., Yano, K., and Nozawa, Y., 1987, Guanosine 5ā²-(Ī³-thio)triphosphate stimulates arachidonic acid liberation in permeabilized rat peritoneal mast cells, Biochem . Biophys. Res. Commun . 145:1267ā1275.
Ragab-Thomas, J. M. F., Hullin, F., Chap, H., and Douste-Blazy, L., 1987, Pathways of arachidonic acid liberation in thrombin and calcium ionophore A23187-stimulated human endothelial cells: Respective roles of phospholipids and triacylglycerol and evidence for diacylglycerol generation from phosphatidylcholine, Biochim . Biophys. Acta 917:388ā397.
Schacht, J., 1976, Inhibition of neomycin of polyphosphoinositide turnover in subcellular fractions of guinea-pig cerebral cortex in vitro, J. Neurochem. 27:1119ā1124.
Smith, J. B., Dangelmaier, C., and Mauco, G., 1985, Measurement of arachidonic acid liberation in thrombin-stimulated human platelets. Use of agents that inhibit both the eyelooxygenƤse and lipoxygenase enzymes, Biochim . Biophys. Acta 835:344ā351.
Sutherland, C. A., and Amin, D., 1982, Relative activities of rat and dog platelet phospholipase A2 and diglyeride lipase, J. Biol. Chem. 257:14006ā14010.
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Ā© 1989 Plenum Press, New York
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Okano, Y., Nakashima, S., Tohmatsu, T., Yamada, K., Nagata, KI., Nozawa, Y. (1989). Guanine Nucleotide-Dependent Release of Arachidonic Acid in Permeabilized Inflammatory Cells. In: Fiskum, G. (eds) Cell Calcium Metabolism. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5598-4_25
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DOI: https://doi.org/10.1007/978-1-4684-5598-4_25
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