Abstract
The calcium pump in liver plasma membrane, which is supported by a high-affinity calcium-activated ATPase (Lotersztajnet al., 1981, 1982; Pavoineet al., 1987), is responsible for extrusion of calcium out of the cell. We have shown that the liver Ca2+ pump is specifically inhibited by pharmacological concentrations of glucagon (Lotersztajnet al., 1984, 1985). This inhibition is independent of adenylate cyclase activation (Lotersztajnet al., 1984) and is probably unrelated to activation of phospholipase C by nanomolar concentrations of glucagon (Wakelamet al., 1986). The liver Ca2+ pump is also regulated by two proteins, an activator that directly stimulates activity of the purified (Ca2+ —Mg2+ ) ATPase (Lotersztajnet al., 1981), and a 30,000-Da inhibitor that interacts with the purified enzyme only in the presence of the activator and Mg2+ (Lotersztajn and Pecker, 1982) and that mediates enzyme inhibition by glucagon (Lotersztajnet al ., 1985). The question arose as to the possible analogy between these activator and inhibitor proteins of (Ca2+ —Mg2+ )ATPase and guanine nucleotide-binding (G) proteins.
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References
Aksamit, R. P., Backlund, P. S. Jr., and Cantoni, G. L., 1985, Cholera toxin inhibits chemotaxis by a cAMP-independent mechanism, Proc. Nad. Acad. Sci. USA 82:7475–7479.
Birnbaumer, L., Codina, J., Mattera, R., Cerione, R. A., Hildebrandt, J. D., Sunyer, T., Rojas, F. J., Caron, M. G., Lefkowitz, R. J., and Iyengar, R., 1985, Regulation of hormone receptors and adenylyl cyclases by guanine nucleotide binding N proteins, Recent Prog. Hormone Res. 41: 41–99.
Bockaert, J., Deterre, P., Pfister, C., Guillon, G., and Chabre, M., 1985, Inhibition of hormonallyregulated adenylate cyclase by the beta gamma subunit of transducin, EMBO J. 4:1413–1417.
Bourne, H. R., 1986, One molecular machine can transduce diverse signals, Nature 321:814–816.
Cerione, R. A., Codina, J., Kilpatrick, B. F., Staniszewski, C., Gierschik, P., Somers, R. L., Spiegel, A. M., Birnbaumer, L., Caron, M. G., and Lefkowitz, R. J., 1985, Transducin and inhibitory nucleotide regulatory protein inhibit the stimulatory nucleotide regulatory protein mediated stimulation of adenylate cyclase in phospholipid vesicle systems, Biochemistry 24:4499–4503.
Georges, S. K., Uttenthal, L. O., Ghiglione, M., and Bloom, S. R., 1985, Molecular forms of glucagonlike peptides in man, FEBS Lett. 192:275–278.
Gilman, A. G., 1984, G Protein and dual control of adenylate cyclase, Cell 36:577–579.
Heyworth, C. M., Whetton, A. D., Wong, S., Martin, B. R., and Houslay, M. D., 1985, Insulin inhibits the cholera-toxin-catalysed ribosylation of a Mr-25000 protein in rat liver plasma membranes, Biochem. J. 228:593–603.
Imboden, J. B., Shoback, D. M., Pattison, G., and Stobo, J. D., 1986, Cholera toxin inhibits the T cell antigen receptor mediated increase in inositol trisphosphate and cytoplasmic free calcium, Proc. Nad. Acad. Sci. USA 83:5673–5677.
Kallner, A., 1975, Determination of phosphate in serum and urine by a single step malachite-green method, Clin. Chim. Acta. 59:35–39.
Levitski, A., 1987, Regulation of adenylate cyclase by hormones and G proteins, FEBS Lett. 211: 113–118.
Lotersztajn, S., and Pecker, F., 1982, A membrane-bound protein inhibitor of high affinity Ca ATPase in rat liver plasma membranes, J. Biol. Chem. 257:6638–6641.
Lotersztajn, S., Hanoune, J., and Pecker, F., 1981, A high affinity calcium-stimulated magnesium dependent ATPase in rat liver plasma membranes, J. Biol. Chem. 256:11209–11215.
Lotersztajn, S., Mavier, P., Clergue, J., Dhumeaux, D., and Pecker, F., 1982, Human liver plasma membrane Ca ATPase: Identification and sensitivity to calcium antagonists, Hepatology 2:843–848.
Lotersztajn, S., Epand, R., Mallat, A., and Pecker, F., 1984, Inhibition by glucagon of the calcium pump in liver plasma membranes, J. Biol. Chem. 259:8195–8201.
Lotersztajn, S., Mallat, A., Pavoine, C., and Pecker, F., 1985, The inhibition of liver plasma membrane (Ca2+ -Mg2+ )ATPase, J. Biol. Chem. 260:9692–9698.
Lotersztajn, S., Pavoine, C., Mallat, A., Stengel, D., Insel, P. A., and Pecker, F., 1987, Cholera toxin blocks glucagon-mediated inhibition of the liver plasma membrane (Ca2+ -Mg2+ )ATPase, J. Biol. Chem. 262:3114–3117.
Maguire, M. E., and Erdos, J. J., 1980, Inhibition of magnesium uptake by ß-adrenergic agonists and prostaglandin E1 is not mediated by cAMP, J. Biol. Chem. 255:1030–1035.
Mallat, A., Pavoine, C., Lotersztajn, S., and Pecker, F., 1985, Inhibition of the Ca pump in liver plasma membranes by glucagon is due to a metabolite of the hormone, Fed. Proc. 44:1392.
Mallat, A., Pavoine, C., Dufour, M., Lotersztajn, S., Bataille, D., and Pecker, F., 1987, A glucagon fragment is responsible for inhibition of the liver Ca2+ pump by glucagon, Nature 325:620–622.
Neville, D. M., 1968, Isolation of an organ specific protein antigen from cell-surface membrane of rat liver, Biochim. Biophys. Acta. 154:540–552.
Patzelt, C., and Schiltz, E., 1984, Conversion of proglucagon in pancreatic alpha cells: The major end products are glucagon and a single peptide, the major proglucagon fragment that contains two glucagon sequences, Proc. Natl. Acad. Sci. USA 81:5007–5011.
Pavoine, C., Lotersztajn, S., Mallat, A., and Pecker, F., 1987, The high affinity (Ca2+ -Mg2+ )ATPase in liver plasma membranes is a Ca2+ pump, J. Biol. Chem. 262:5113–5118.
Spiegel, A. M., 1987, Signal transduction by guanine nucleotide binding proteins, Mol. Cell Endocrinol. 49:1–16.
Steiner, D. F., Quinn, P. S., Chan, S. J., Marsh, J., and Tager, H. S., 1980, Processing mechanism in the biosynthesis of proteins, Ann. N.Y. Acad. Sci. 343:1–16.
Taylor, C. W., and Merrit, J. E., 1986, Receptor coupling to polyphosphoinositine turnover: A parallel with the adenylate cyclase system, Trends Pharmacol. Sci. 7:238–242.
Wakelam, M. J. O., Murphy, G. J., Hruby, G. J., and Houslay, M. D., 1986, Activation of two signal transduction systems in hepatocytes by glucagon, Nature 323:68–71.
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© 1989 Plenum Press, New York
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Lotersztajn, S., Pavoine, C., Mallat, A., Stengel, D., Insel, P., Pecker, F. (1989). Hormonal Inhibition of the Liver Plasma Membrane (Ca2+ —Mg2+ ) ATPase is Mediated by a Gs-like Protein. In: Fiskum, G. (eds) Cell Calcium Metabolism. GWUMC Department of Biochemistry Annual Spring Symposia. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5598-4_1
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DOI: https://doi.org/10.1007/978-1-4684-5598-4_1
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