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Construction of a Safe and Efficient Retrovirus Packaging Cell Line

  • Dina Markowitz
  • Stephen Goff
  • Arthur Bank
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 34)

Abstract

Ecotropic and amphotropic retrovirus packaging Cell lines have been constructed in which the helper virus genome have been separated onto two plasmids, and the ψ packaging signal and 3’ LTR have been removed. The gag and pol genes on one plasmid and the env gene on another plasmid were transfected into NIH 3T3 Cells. Packaging Cell lines produced by these transfected genes released titers of replication-defective retroviral vectors which were comparable to titers produced by packaging Cell lines containing the helper virus genome on one plasmid. There has been no evidence of recombination events between the ecotropic helper virus plasmids and the vector virus plasmid that would result in the generation of intact replication-competent virus. These results suggest that a packaging Cell line containing gag, pol and env on different plasmids is efficient and safe for use in retroviral gene gransfer.

Key Words

Retroviral gene transfer — packaging Cell line. 

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Dina Markowitz
    • 1
    • 2
    • 3
  • Stephen Goff
    • 1
    • 2
    • 3
  • Arthur Bank
    • 1
    • 2
    • 3
  1. 1.Department of Genetics and Development College of PhysiciansColumbia UniversityNew YorkUSA
  2. 2.Department of Biochemistry and Molecular Biophysics College of Physicians and SurgeonsColumbia UniversityNew YorkUSA
  3. 3.Department of Medicine College of Physicians and SurgeonsColumbia UniversityNew YorkUSA

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