Abnormalities of the Hematopoietic Regulatory Network

  • Grover C. BagbyJr.
  • Brenda Wilkinson
  • Elaine McCall
  • Melinda Lee
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 34)


It is now widely recognized that mononuclear phagocytes play a major role in regulating the production of hematopoietic growth factors by a variety of cell types1–17. As shown in Fig. 1, two monokines, interleukin 1 (IL-1) and tumor necrosis factor alpha (TNF) are known to induce the expression of G- and GM-CSF genes in fibroblasts and in endothelial cells7,9,13,15 . Such observations have supported our notion that mononuclear phagocytes may serve as pivotal regulatory cells in growth factor gene expression12. The principal hurdle to be overcome is the uncertainty surrounding the biological relevance of these findings. This is a legitimate concern in view of the highly reductionistic design of the studies used in support of this model. For example, studies in our laboratory begin with the purification and culture, independently of other collaborating cell populations, of peripheral Blood monocytes in complete tissue culture medium to generate monocyte conditioned medium (MCM), followed by the introduction of serial dilutions of that MCM to confluent cultures of stromal cells such as fibroblasts or endothelial cells, and the assay of the stromal cell supernatants for hematopoietic growth factors1–4,6,11,14,15. In such experiments, IL-1 secreted by mononuclear phagocytes, induces the expression of G- and GM-CSF genes7,9,15, a phenomenon which can be detected by using the fibroblast or endothelial cell supernatants in specific bioassays with neutralizing antibodies15, by using western blot analysis, and by measuring CSF gene transcripts by northern or Si nuclease analysis7,9,15 (Fig. 2).


Mononuclear Phagocyte Colony Growth Elderly Volunteer Hematopoietic Growth Factor Auxiliary Cell 
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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Grover C. BagbyJr.
    • 1
    • 2
    • 3
    • 4
    • 5
  • Brenda Wilkinson
    • 1
    • 2
    • 3
    • 4
    • 5
  • Elaine McCall
    • 1
    • 2
    • 3
    • 4
    • 5
  • Melinda Lee
    • 1
    • 2
    • 3
    • 4
    • 5
  1. 1.Medical Research, VA Medical CenterOregon Health Sciences UniversityPortlandUSA
  2. 2.Division of HematologyOregon Health Sciences UniversityPortlandUSA
  3. 3.Medical Research, VA Medical Center and the Divisions of Hematology and Medical Oncology and Geriatrics Department of MedicineOregon Health Sciences UniversityPortlandUSA
  4. 4.Division of Medical Oncology and GeriatricsOregon Health Sciences UniversityPortlandUSA
  5. 5.Department of MedicineOregon Health Sciences UniversityPortlandUSA

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