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Devitrification and Recrystallization of Glass Forming Aqueous Solutions

  • Conference paper
Book cover The Biophysics of Organ Cryopreservation

Part of the book series: NATO ASI Series ((NSSA,volume 147))

Abstract

The ideal aqueous solution for use in cryobiology would be one which was completely non-toxic and which could be cooled to liquid nitrogen temperatures and returned to room temperature without the formation of ice or any other crystalline phase. Unfortunately requirements of non-toxicity and non-freezing are rarely compatible and the cryobiological techniques which have been developed represent a variety of compromises. On one extreme is the technique known as cryopreservation by vitrification (1,2,3). In this technique large weight fractions of solute are added to the cryopreservation solution and the solution forms a glass on cooling, but the solutes are usually toxic to some extent at the high concentrations involved. Given the toxicity problem, the concentration of solutes is usually kept to the minimum needed for glass formation and as a result the glass usually forms ice briefly during warming. Hence ice is not totally avoided. At the other extreme (4), the solutions are loaded with only relatively minor amounts of solute and the solution inevitably forms a large, approaching equilibrium, quantity of ice during the cooling and heating excursion.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Monash University, Clayton, Victoria, 3168, Australia

    Douglas R. MacFarlane & Maria Forsyth

Authors
  1. Douglas R. MacFarlane
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  2. Maria Forsyth
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Editor information

Editors and Affiliations

  1. MRC Medical Cryobiology Group, Cambridge University, Cambridge, UK

    David E. Pegg

  2. Medical College of Georgia, Augusta, Georgia, USA

    Armand M. Karow Jr.

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© 1987 Plenum Press, New York

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MacFarlane, D.R., Forsyth, M. (1987). Devitrification and Recrystallization of Glass Forming Aqueous Solutions. In: Pegg, D.E., Karow, A.M. (eds) The Biophysics of Organ Cryopreservation. NATO ASI Series, vol 147. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5469-7_12

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  • DOI: https://doi.org/10.1007/978-1-4684-5469-7_12

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5471-0

  • Online ISBN: 978-1-4684-5469-7

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