Abstract
The mechanisms by which gonadotropins regulate the development and function of the corpus luteum are not completely understoon. Cyclic AMP is recognized as the primary ‘second messenger’ of luteinizing hormone (LH) action in the corpus luteum. We have recently reported that gonadotropins also stimulate the accumulation of ‘second messengers’ derived from phospholipase C (PLC) catalysed hydrolysis of phosphatidylinositol 4,5-bisphosphate (Davis et al., 1986a,c, 1987). Hydrolysis of this phospholipid liberates diacylglycerol and inositol 1,4,5-trisphosphate (IP 3) which serve to activate protein kinase C (Nishizuka, 1986) and mobilize intracellular Ca2 (Berridge, 1984), respectively. This newly described ‘second messenger’ system may be responsible for regulating cellular growth, metabolism and secretion in hormone, growth factor and neurotransmitter responsive tissues. The present studies were performed to examine the effect of LH on intracellular Ca concentrations in isolated bovine luteal cells. LH-induced increases in the levels of IP3 and cAMP were correlated with increases in intracellular Ca2+.
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References
Bedwani JR, Wong PYD, 1975. Ionic dependence of luteinizing hormone-induced steroidogenesis in the rabbit ovary. J Physiol 250:669–679
Berridge MJ, 1984. Inositol trisphosphate and diacylglycerol as second messengers. Biochem J 220:345–360
Clark MR, Kawai Y, Davis JS, LeMaire WJ, 1985. Ovarian protein kinases. In: Toft DO, Ryan RJ (eds), Proceedings of the Fifth Ovarian Workshop. Illinois: Ovarian Workshops, pp. 383–401
Davis JS, Clark MR, 1983. Activation of protein kinase in the bovine corpus luteum by phospholipid and Ca2+ . Biochem J 214:569–574
Davis JS, Farese RV, Marsh JM, 1981. Luteinizing hormone stimulates phospholipid labeling and progesterone synthesis in isolated bovine luteal cells. Endocrinology 109:469–475
Davis JS, Weakland LL, Farese RV, West LA, 1987. Luteinizing hormone increases inositol trisphosphate and cytosolic free Ca2+ in isolated bovine luteal cells. J Biol Chem, in press
Davis JS, Weakland LL, West LA, Farese RV, 1986a. Luteinizing hormone stimulates the formation of inositol trisphosphate and cyclic AMP in rat granulosa cells: evidence for phospholipase C generated second messengers in the action of luteinizing hormone. Biochem J 238:597–604
Davis JS, West LA, Farese RV, 1986b. Gonadotropin-releasing hormone (GnRH) rapidly stimulates the formation of inositol phosphates and diacylglycerol in rat granulosa cells: further evidence for the involvement of Ca2 and protein kinase C in the action of GnRH. Endocrinology 118:2561–1571
Davis JS, West LA, Weakland LL, Farese RV, 1986c. Human chorionic gonadotropin activates the inositol l,4,5-trisphosphate-Ca2+ intracellular signalling system in bovine luteal cells. FEBS Lett 208:287–291
Dorflinger LJ, Albert PJ, Williams AT, Behrman HR, 1984. Calcium is an inhibitor of luteinizing hormone-sensitive adenylate cyclase in the luteal cell. Endocrinology 114:1208–1215
Hansel W, Dowd JP, 1986. New concepts of the control of corpus luteum function. J Reprod Fert 78:755–768
Higuchi T, Kaneko A, Abel JH Jr, Niswender GD, 1976. Relationship between membrane potential and progesterone release in ovine corpora lutea. Endocrinology 99:1023–1032
Lenz RW, Ax RL, First NL, 1982. Proteoglycan production by bovine granulosa cells in vitro is regulated by calmodulin and calcium. Endocrinology 110:1052–1054
Nishizuka Y, 1986. Studies and perspectives of protein kinase C. Science 233:305–312
Sawyer HR, Abel JH Jr, McMlellan MC, Schmitz M, Niswender GD, 1979. Secretory granules and progesterone secretion by ovine corpora lutea in vitro. Endocrinology 104:476–486
Shemesh M, Hansel W, Strauss JF, III, 1984. Calcium-dependent, cyclic nuc-leotide-independent steroidogenesis in the bovine placenta. Proc Natl Acad Sci USA 81:6403–6407
Tsien RY, Pozzan T, Rink TJ, 1982. Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new intracellularly trapped fluorescent indicator. J Cell Biol 94:325–344
Veldhuis JD, Hammond JM, 1981. Role of calcium in the modulation of ornithine decarboxylase activity in isolated pig granulosa cells in vitro. Biochem J 196:795–801
Veldhuis JD, Klase PA, Demers LM, Chafouleas JG, 1984. Mechanisms subserving calcium’s modulation of luteinizing hormone action in isolated swine granulosa cells. Endocrinology 114:441–449
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© 1987 Plenum Press, New York
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Davis, J.S. (1987). Stimulation of Intracellular Free Ca2+ by Luteinizing Hormone in Isolated Bovine Luteal Cells. In: Mahesh, V.B., Dhindsa, D.S., Anderson, E., Kalra, S.P. (eds) Regulation of Ovarian and Testicular Function. Advances in Experimental Medicine and Biology, vol 219. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5395-9_40
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DOI: https://doi.org/10.1007/978-1-4684-5395-9_40
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