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Recognition of DNA Sequences by Restriction Endonucleases

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DNA—Ligand Interactions

Part of the book series: NATO ASI Series ((volume 137))

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Abstract

Type II restriction endonucleases recognize DNA sequences 4 or 6 basepairs long with very high specificity in a vast excess of non-specific DNA binding sites. They cleave the DNA within or in the immediate vicinity of the recognition sequence. Because of the immense importance of these enzymes in analysis, preparation and in vitro recombination of DNA many investigations have been carried out to understand the structural and mechanistic features underlying their mode of recognition1. Among approximately 500 type II restriction endonucleases described, EcoRI is the so far best studied enzyme. EcoRI is a dimeric protein with two identical sub-units, the aminoacid sequence of which is known2,3.

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Authors and Affiliations

  1. Biophysikalische Chemie, Med. Hochschule Hannover, D-3000, Hannover 61, Germany

    Guenter Maass

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  1. Guenter Maass
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Editors and Affiliations

  1. Service de Biochimie, Bat. 142, Département de Biologie, Centre d’Etudes Nucléaires de Saclay, F-91191, Gif-sur-Yvette Cedex, France

    Wilhelm Guschlbauer (Director) (Director)

  2. Institut für Kristallographie, Freie Universität, Takustrasse 6, D-1000, Berlin 33, German Federal Republic

    Wolfram Saenger (Codirector) (Codirector)

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© 1987 Plenum Press, New York

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Maass, G. (1987). Recognition of DNA Sequences by Restriction Endonucleases. In: Guschlbauer, W., Saenger, W. (eds) DNA—Ligand Interactions. NATO ASI Series, vol 137. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5383-6_16

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  • DOI: https://doi.org/10.1007/978-1-4684-5383-6_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5385-0

  • Online ISBN: 978-1-4684-5383-6

  • eBook Packages: Springer Book Archive

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