Abstract
A high percentage of gut lamina propria plasma cells in tissue sections were stained by three fluoresceinated rabbit anti mouse IgG3 antisera. Similar cytoplasmic staining was found in several mouse strains including CBA/N mice. To further extend these data, northern hybridization experiments with a cDNA probe specific for IgG3 was performed with total RNA isolated from the small intestine or from cell preparations enriched for lamina propria plasma cells (data not shown). In contrast to the fluorescent studies, little or no mRNA for IgG3 was found. In all cases the antigen used in producing the anti-mouse IgG3 serum was the J 606 (IgG3) myeloma protein, which reacts with certain polysaccharides such as inulin [β(2–1) poly fructosan] and bacterial levan [β(2–6) polyfructosan].
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Mosier, D.E. and Subbarao, B., Immunol. Today 3 217, 1982.
Johnson, N., Slankard, H., Paul, L. and Hood, L., J. Immunol 128, 302, 1982.
Lieberman, R., Potter, M., Humphrey, W., Mushinski, E.B. and Vrana, M., J. Exp. Med. 142, 106, 1975.
Basta, P., Kubagawa, H., Kearney, J.F. and Briles, D.E., J. Immunol. 130, 2423, 1983.
Slack, J., Der Balian, G., Nahm, M. and Davie, J.M., J. Exp. Med. 151, 853, 1980.
Ohriner, W.D. and Cebra, J.J., Eur. J. Immunol. 15, 906, 1985.
Teale, J.M., J. Immunol. 130, 72, 1983.
Eldridge, J.H., Kiyono, H., Michalek, S.M. and McGhee, J.R., J. Exp. Med. 157, 789, 1983.
Brodeur, P.H. and Riblet, R., Eur. J. Immunol. 14, 92, 1984.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Andre, P.M., Tomasi, T.B. (1987). A Predominant Idiotype in the Gut Associated Lymphoid Tissue. In: Mestecky, J., McGhee, J.R., Bienenstock, J., Ogra, P.L. (eds) Recent Advances in Mucosal Immunology. Advances in Experimental Medicine and Biology, vol 216 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5344-7_19
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5344-7_19
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5346-1
Online ISBN: 978-1-4684-5344-7
eBook Packages: Springer Book Archive