Methods and Applications of Restriction Fragment Length Polymorphism Analysis to Plants

  • Benoit S. Landry
  • Richard W. Michelmore
Part of the Basic Life Sciences book series (BLSC, volume 41)


The lack of markers has hindered genetic analyses in many important plant species. Molecular biology now provides an opportunity to develop large numbers of genetic markers. Cloned DNA sequences can be used to probe specific regions of eukaryotic genomes for the presence of polymorphism at the DNA sequence level. This polymorphism is detected as variation in the length of DNA fragments homologous to a labeled probe after digestion of the genomic DNA with restriction endonucleases. Such variation has been termed restriction fragment length polymorphism (RFLP).


Restriction Fragment Length Polymorphism Human Leukocyte Antigen Class Polytene Chromosome Restriction Fragment Length Polymorphism Analysis Restriction Fragment Length Polymorphism Marker 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Benoit S. Landry
    • 1
  • Richard W. Michelmore
    • 1
  1. 1.Department of Vegetable CropsUniversity of CaliforniaDavisUSA

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