Genetic Tools for the Analysis of Gene Expression in Plants
- 272 Downloads
The analysis of gene expression in plants is often based on correlations between data obtained by a variety of means: biochemical, physiological, and molecular genetic studies on the one hand, and classical genetic tools on the other hand. Among the techniques applied to the study of plant genes are screening of various genomic and complementary DNA (cDNA) libraries by synthetic oligonucleotides, gene- or organ-specific cDNAs, or antibody probes, in vitro translation of hybrid-released mRNAs, two-dimensional protein gel electrophoresis, immunoblotting, and transcript mapping (7,10). However, for an in depth understanding of how certain genes are regulated, alternative approaches are needed.
KeywordsGene Fusion Chimeric Gene Selectable Marker Gene Chloramphenicol Acetyl Transferase Target Plasmid
Unable to display preview. Download preview PDF.
- 7.Dodds, J.H., ed. (1985) Plant Genetic Engineering, Cambridge University Press, Cambridge, England.Google Scholar
- 10.Freeling, M., ed., (1985) Plant Genetics (UCLA Symposia on Molecular and Cellular Biology, New Series Vol. 35 ), Alan R. Liss, Inc., New York.Google Scholar
- 13.Hastings, J.W., T.O. Baldwin, and M.Z. Nicoli, (1978) Bacterial luciferase: Assay, purification and properties. In Bioluminescence and Chemiluminescence (Methods in Enzymology, Vol. LVII), M.A. DeLuca, ed. Academic Press, Inc., New York, pp. 135–152.Google Scholar
- 21.Koncz, C., Zs. Koncz-Kalman, and J. Schell, (1986) Transposon Tn5 mediated gene transfer into plants. Mol. Gen. Genet. (in apress).Google Scholar
- 22.Koncz, C., F. Kreuzaler, Zs. Kalman, and J. Schell, (1984) A simple method to transfer, integrate, and study expression of foreign genes, such as chicken ovalbumin and a-actin in plant tumors. EMBO J. 3: 1597–1603.Google Scholar
- 23.Koncz, C., O. Olsson, W.H.R. Langridge, J. Schell, and A. Szalay, (1986) Expression and functional assembly of bacterial luciferase in plants. Proc. Natl. Acad. Sci., USA (in apress).Google Scholar
- 27.Saedler, H., U. Bonas, A. Gierl, B.J. Harrison, R.B. Klösgen, E. Krebbers, P. Nevers, P.A. Peterson, Zs. Schwarz-Sommer, H. Sommer, K. Upadhyaya, and U. Wienand, (1984) Transposable elements in Antirrhinum majus and Zea mays. Cold Spring Harbor Symp. Quant. Biol. 49: 355–361.PubMedCrossRefGoogle Scholar
- 32.Stachel, S.E., E. Messens, M. Van Montagu, and P. Zambryski, (1985) Identification of signal molecules produced by wounded plant cells that activate T-DNA transfer. in Agrobacterium tumefaciens. Nature 318: 624–630.Google Scholar
- 34.Vasil, I.K., ed., (1984) Cell Culture and Somatic Cell Genetics of Plants, Vol. 1, Academic Press, Inc., New York.Google Scholar