Abstract
Cell biologists, biochemists, biophysicists, and other life scientists who study the mammalian cell are in general concerned with the basic questions, What is the cell made of? How does it work? In order to answer these questions, scientists are continuing to develop new techniques and approaches which are revealing a variety of information. It is interesting that some of this information remains buried, only to be rediscovered a decade or so later as new or even old models of cell structure and function are proposed (see Conklin, 1940, for an historical review).
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References
Caille, J.P., and Hinke, J.A.M., 1974, The volume available to diffusion in the muscle fiber, Qam Physiol Pharmacol, 52:814.
Clegg, J.S,, 1984, Properties and metabolism of the aqueous cytoplasm and its boundaries, Am. J. Physiol,. 246: R133.
Conklin, E.G., 1940, Cell and protoplasm concepts: historical account, In: The Cell and Protoplasm, F.R. Moulton, ed., The Science Press, Washington, D. C.
Crick, F.H.C., and Hughes, A.F.W., 1950, The physical properties of cytoplasm: a study by means of the magnetic particle method, Exp. Cell Res., 1:37.
Fulton, A.B., 1982, How crowded is the cytoplasm? Cell. 30:345.
Geiger, B., Kreis, T.E., Avnur, Z. and Schlessinger, J., Cell and Muscle Motility, 1985, in press.
Gershon, N., Porter, K. and Trus, B., 1983, The micro trabecular lattice and the cytoskeleton, their volume, surface area and the diffusion of molecules through it, In: Biological Structures and Coupled Flows, A. Oplatka and M. Balaban, eds., Academic Press, New York.
Hammerstedt, R.H., Keith, A.D., Boltz, R.C., and Todd, P.W., 1979, Use of amphiphilic spin labels and whole cell isoelectric focusing to assay charge characteristics of sperm surfaces, Arch. Biochem. Biophys., 194:565.
Hodgkin, A.L., and Keynes, R.D., 1956, Experiments on the injection of substances into squid giant axons by means of a micro syringe, J. Phygiol., 131:592.
Horowitz, S.B., 1972, The permeability of the amphibian oocyte nucleus, J. Cell Biol., 54:609.
Horowitz, S.B., and Moore, L.E., 1974, The nuclear permeability, intracellular distribution, and diffusion of insulin in the amphibian oocyte, J. Cell Biol., 60:405.
Horowitz, S.B., Fenichel, I.R., Hoffman, B., Kollmann, G., and Shapiro, B. 1970, The intracellular transport and distribution of cysteamine phosphate derivatives, Biophvs. 10:944.
Jacobson, K. and Wojcieszyn, J., 1984, The translational mobility of substances within the cytoplasmic matrix, Proc. Natl. Acad. Sci. USA, 81:6747.
Keith, A.D. and Mastro, A.M., Cell and water viscosity, in Biomembranes, Methods in Enzvmology, L. Packer, ed., in press.
Keith, A.D., and Snipes, W., 1974, Viscosity of cellular protoplasm, Science (Wash. DC), 183:666.
Keith, A.D., Mastro, A.M., and Snipes, W., 1979, Diffusion relationships between cellular plasma membrane and cytoplasm, In: Low Temperature Stress in Crop Plants, J. Lyons and J. Raison, eds., Academic Press, New York.
Keith, A.D., Snipes, W., and Chapman, D., 1977, Spin label studies on the aqueous regions of phospholipid multilayers, Biochemistry, 16:634.
Keith, A.D., Snipes, W.C., Melhorn, R.J., and Gunter, T., 1977, Factors restricting diffusion of water-soluble spin labels, Biophvs. J., 19:205.
Kreis, T.E., Geiger, B., and Schlessinger, J., 1982, Mobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery, Cell, 29:835.
Mansell, J., and Clegg, J.S., 1983, Cellular and molecular consequences of reduced cell water content, Crvobiology., 20:591.
Mastro, A.M. and Pepin, K.G., 1980, Supressiono of lectin-stimulated DNA synthesis in bovine lymphocytes by the tumor promoter 12–0- tetradecanoyl phorbol-13-acetate, Cancer Res., 40:3307.
Mastro, A.M. and Keith, A.D., 1984, Diffusion in the aqueous compartment, J. Cell Biol., 99:180s.
Mastro, A., and Keith, U, 1981, Spin label viscosity studies of mammalian cell cytoplasm, In: The Transformed Cell, I. Cameron and T. Pool, eds., Academic Press, New York.
Mastro, A.M., Babich, M., Taylor, W., and Keith, A., 1984, Diffusion of a small molecule in the cytoplasm of mammalian cells, Proc. Natl. Acad. Sci. USA. 81:3414.
Paine, P.L. and Horowitz, S.B., 1980, The movement of material between nucleus and cytoplasm,.In: Cell Biology: A Comprehensive Treatise, D.M. Prescott and L. Goldstein, eds., Academic Press, New York.
Porter, K.R. and Tucker, J.B., 1981, The ground substance of living cells, Sci. Am., 244(3):57.
Porter, K.R., Boggs, D.P., and Anderson, K.L., 1982, The distribution of water in the cytoplasm, 40th Annual Proceedings of the Electron Microscope Socioty M lusriss, 4–7.
Schobert, B., and Marsh, D., 1982, Spin label studies on osmotically induced changes in the aqueous cytoplasm of Phaeodacvtvlomr trjcornutup, Biochem., Biophys., Acta., 720:87.
Suzuki, A., Goll, D.E., Singh, I., Allen, R.E., Robson, R.M., and Stromer, M.H., 1976, Some properties of purified skeletal muscle a -actinin, J. Biol. Chem., 251:6860.
Wang, Y., Lanni, F., McNeil, P.L., Ware, B.R., and Taylor, D.L., 1982, Mobility of cytoplasmic and membrane-associated actin in living cells, Proc. Natl. Acad. Sci. USA. 79:4660.
Wolosewick, J. J., and Porter, K.R., 1979, Micro trabecular lattice of the cytoplasmic ground substance, J. Cell Biol., 82:114.
Wojcieszyn, J.W., Schlegel, R.A., Wu, E.S., and Jacobson, K.A., 1981, Diffusion of injected macromolecules within the cytoplasm of living cells, Proc, Natl, Acfrd, Sci, USA, 78:4407.
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© 1986 Plenum Press, New York
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Mastro, A.M., Hurley, D.J. (1986). Diffusion of a Small Molecule in the Aqueous Compartment of Mammalian Cells. In: Welch, G.R., Clegg, J.S. (eds) The Organization of Cell Metabolism. NATO ASI Series, vol 127. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5311-9_6
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DOI: https://doi.org/10.1007/978-1-4684-5311-9_6
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