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Cell Walls and Secondary Products as Obstacles to Plant Enzyme Isolation: Problems and Solutions, Including a Simple Liquid-Nitrogen Homogenizer for Bulk Tissue Extraction

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Book cover The Metabolism, Structure, and Function of Plant Lipids

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Abstract

When we first undertook studies of monoterpene biosynthesis in peppermint (Mentha piperita), we and other plant biochemists were handicapped by inadequate methodology and by inadequate understanding of plants. Gas chromatography was not yet available. It was assumed that secondary metabolites were synthesized slowly and irreversibly, so tracer experiments were conducted over periods of days instead of minutes or hours. Finally, the unique problems of plant enzymology were not understood.

Supported in part by grants from the National Science Foundation and the O.S.U. Environmental Health Sciences Center.

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Author information

Author notes

  1. Richard P. Sandstrom

    Present address: Botany Department, University of Washington, Seattle, Washington, 98195, USA

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, 97331, USA

    Richard P. Sandstrom & W. David Loomis

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  1. Richard P. Sandstrom
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  2. W. David Loomis
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Editor information

Editors and Affiliations

  1. University of California, Davis, Davis, California, USA

    Paul K. Stumpf

  2. ARCO Plant Cell Research Institute, Dublin, California, USA

    J. Brian Mudd

  3. U.S. Department of Agriculture, Albany, California, USA

    W. David Nes

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© 1987 Plenum Press, New York

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Sandstrom, R.P., Loomis, W.D. (1987). Cell Walls and Secondary Products as Obstacles to Plant Enzyme Isolation: Problems and Solutions, Including a Simple Liquid-Nitrogen Homogenizer for Bulk Tissue Extraction. In: Stumpf, P.K., Mudd, J.B., Nes, W.D. (eds) The Metabolism, Structure, and Function of Plant Lipids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5263-1_6

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  • DOI: https://doi.org/10.1007/978-1-4684-5263-1_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5265-5

  • Online ISBN: 978-1-4684-5263-1

  • eBook Packages: Springer Book Archive

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