Abstract
Corey published a leading paper in 196 7 demonstrating that 2, 3-iminosqualene inhibited the cyclization of squalene-oxide to lanosterol in a cell-free system1. In the interim years between then and this seventh international plant lipid symposium, four groups-one each in the United States2, Canada3 another in France4 and Italy4, have firmly established the mechanistic and biological importance of using this and structurally related molecules, e.g. 25-azasteroids5, to interfere with fungal sterol biosynthesis which results in diminished growth response. Industry and biotechnology firms have followed suit in recent years designing analogous inhibitors6, 7 which they believe may have potential promise in plant protection. On the premise that specific N-isopentenoids induce alterations in sterol biosynthesis which in turn may create a pathologic state in the structure and function of membranes of pathogenic fungi, we began a chemical synthesis program in 1984 by preparing blockers targeted at fungal lipid biosynthesis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
E.J. Corey, P.R.O. de Montellano, K. Lin, and P.D. 2, 3-Iminosqualene, a potent inhibitor of the enzymc cyclization of 2, 3-oxidosqualene to sterols. J. Amer. Chem. Soc. 89/2797 (1967).
W.J. Pinto and W.R. Nes, Stereochemical specificity of sterols in Saccharomyces cerevisiae. J. Biol. Chem. 258: 4472 (1983).
L. Avruch and A.C. Oehlschlager, A direct synthesis of 2, 3-epiminosqualene synthesis 10/622 (1973).
A. Rahier, M. Taton, P. Bouvier-Nave, P. Schmitt, P. Benveniste, F. Schuber, A.S. Narula, L. Cattel, C. Anding and P. Place, Design of high energy intermediate analogues to study sterol biosynthesis in higher plants. Lipids 21: 52 (1986).
A.C. Oehlschlager, R.H. Angus, A.M. Pierce, H.D. Pierce and R. Srinivasan, Azasterol inhibition of Δ24 sterol methyltransferase in Saccharomyces cerevisiae. Biochemistry 23: 3582 (1984).
F. Paltauf, G. Daum, G. Zuder, G. Hogenauer, G. Schulz, and G. Seidl. Squalene and ergosterol biosynthesis in fungi treated with natifine, a new antimycotic agent. Biochim. Biophys. Acta 712: 268 (1982).
W.D. Nes, P.H. Le, L.R. Berg, G.W. Patterson and J.L. Kerwin, A comparison of cycloartenol and lanosterol biosynthesis and metabolism by the Oomycetes. Experientia 42: 556 (1986).
W.D. Nes and A.E. Stafford, Evidence for metabolic and functional discrimination of sterols by Phytophthora cactorum. Proc. Natl. Acad. Sci. 80: 3227 (1983).
W.D. Nes and R.C. Heupel, Physiological requirement for biosynthesis of multiple 24ß-methylsterols in Gibberella fujikuroi. Arch. Biochem. Biophys. 244: 211 (1986).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Parish, E.J., Hanners, P.K., Nes, W.D. (1987). Synthesis and Biological Evaluation of Fungal Bioregulators of Sterol Biosynthesis. In: Stumpf, P.K., Mudd, J.B., Nes, W.D. (eds) The Metabolism, Structure, and Function of Plant Lipids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5263-1_15
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5263-1_15
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5265-5
Online ISBN: 978-1-4684-5263-1
eBook Packages: Springer Book Archive