Summary
In the process of the decoding of the mRNA by the 70 S ribosome a hexanucleotide double stranded RNA is formed by the mRNA and the aminoacyl- and the peptidyl-tRNA. Both, in initiation or termination, however, only either the formylmethionylor the peptidyl-tRNA are present on the ribosome. According to a theory as outlined by Shine and Dalgarno the 3’ end of the 16 S RNA can form a short double strand with the mRNA. In order to prove these assumptions we prepared oligonucleotides containing the Shine-Dalgarno region and the initiation codon. These were examined in their capabilities to direct the binding of fMet-tRNA to the 30 S ribosome. The data indicate that the double strand formation between 16 S RNA and mRNA acts as a signal to stimulate the binding of the initiator-tRNA to the ribosomal subunit.
To elucidate details of the termination reaction the binding constants between the release factors and the codons UAA, UAG and UGA have been determined. Furthermore, we crosslinked the codons to the 70 S ribosomal termination complex. The nucleotides were crosslinked to both the proteins and the 16 S RNA. The data suggest that the termination codons interact with the 16 S RNA. A model, which explains the function of the 16 S RNA in initiation and elongation is proposed.
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References
Backendorf, C., Overbeek, G. P., Van Boom, J. H., Van Der Marel, G., Veeneman, G. and Van Duin, J., 1980, Role of 16 S RNA in Ribosome Messenger Recognition, Eur. J. Biochem., 110: 599
Caskey, C. Th., 1980, Peptide Chain Termination, Trends Biochem. Sci., 5: 234
Gassen, H. G. 1982, The Bacterial Ribosome: A Programmed Enzyme, Angew. Chemie, 21: 23
Holschuh, K., Riesner, D. and Gassen, H. G., 1981, Steps of mRNA Translocation in Protein Biosynthesis, Nature, 293: 675
Holschuh, K. and Gassen, H. G., 1982, Mechanism of Translocation: Binding Equilibria Between the Ribosome, mRNA Analogues and Cognate tRNAs, J. of Biol. Chem., 257: 1987
Schmitt, M., Manderschied, U., Kyriatsoulis, A., Brinckmann, U. and Gassen, H. G., 1980, Tetranucleotides as Effectors for the Binding of Initiator tRNA to Escherichia coli Ribosomes, Eur. J. Biochem., 109; 291
Shine, J. and Dalgarno, L., 1974, The 3’ Terminal Sequence of Escherichia coli 16 S Ribosomal RNA: Complementary to Nonsense Triplets and Ribosome Binding Sites, Proc. Natl. Acad. Sci. USA, 71: 1342
Steitz, J. A. and Jakes, K., 1975 How Ribosomes Select Initiator Regions in mRNA: Base Pair Formation Between the 3’ Terminus of 16 S rRNA and the mRNA During Initiation of Protein Syntheses in Escherichia coli, Proc. Natl. Acad. Sci. USA, 72: 4734
Steitz, J. A., 1980, RNA-RNA Interaction During Polypeptide Chain Initiation, in: “Ribosomes, Structure, Function and Genetics”, Craven, G. R., Davies, J., Davies, K., Kahan, L. and Nomura, M., ed., University Park Press, Baltimore
Taniguchi, T. and Weissmann, C., 1978, Inhibition of Qß RNA 70 S Ribosome Initiation Complex Formation by an Oligonucleotide Complementary to the 3’ Terminal Region of Escherichia coli 16 S Ribosomal RNA, Nature, 275: 770
Tate, W. P., Ward, Ch. D., Trotman, N. A. C., Lührmann, R. and Stöffler, G. 1983, The Shine and Dalgarno Hypothesis for Termination: The 3’Terminus of the 16S rRNA of the Escherichia coli Ribosome can be Modified or Base-Paired with a Complementary Oligonucleotide without Affecting Termination in Vitro, Biochem. Int., 7: 529
Tate, W. P., Hornig, H. and Lührmann, R., 1983, Recognition of Termination Codon by Release Factor in the Presence of a tRNA Occupied A-site, J. Biol. Chem., 258: 10360
Tessier, L.-H., Sondermeyer, P., Faure, T., Dreyer, D., Benavente, A., Villeval, D., Courtney, M. and Lecoy, J.-P., 1984, The Influence of mRNA Primary and Secondary Structure on Human IFN-Y-Gene Expression in Escherichia coli, Nucleid Acids Research, 12: 7663
Van Duin, J., Ravensbergen, C. J. C. and Doornbos, J., 1984) Basepairing of Oligonucleotides to the 3’end of 16S Ribosomal RNA is not Stabilized by Ribosomal Proteins, Nucl. Acids Res., 12: 5079
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© 1986 Plenum Press, New York
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Eckert, V., Lang, A., Kyriatsoulis, A., Gassen, H.G. (1986). Double Stranded RNA in the Decoding of the mRNA by the Bacterial Ribosome. In: van Knippenberg, P.H., Hilbers, C.W. (eds) Structure and Dynamics of RNA. NATO ASI Series, vol 110. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5173-3_23
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DOI: https://doi.org/10.1007/978-1-4684-5173-3_23
Publisher Name: Springer, Boston, MA
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