Abstract
We have developed a number of monoclonal antibodies (MoAb) against human sperm. 4 of them have been characterized to some extent. In the indirect immunofluorescence, clone C., reacts with acrogome, Clone F11G1 reacts with the main tail piece, C1one 6SPB6 stains the whole sperm and Clone A3 stains the equatorial segment as well as the entire tail. None of the 4 MoAbs give reaction with human peripheral blood lymphocytes, human erythrocytes or human serum proteins in the sandwich type enzyme immunoassay. MoAb F11 G1 cross-reacts with monkey and baboon sperm but not with mice, rat or hamster epididymal sperm. MoAb 6SPB6 and A3 react with human, monkey and baboon sperm strongly but weakly with rat sperm. However, MoAb C1 was found to react only with human sperm. Their efficacy to prevent fertilization is being tested in vitro in zona-free hamster egg system and in baboons in vivo.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Singh, G. (1986). Monoclonal Antibodies Against Human Sperm. In: Talwar, G.P. (eds) Immunological Approaches to Contraception and Promotion of Fertility. Reproductive Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5140-5_23
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5140-5_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5142-9
Online ISBN: 978-1-4684-5140-5
eBook Packages: Springer Book Archive