Contraceptive Effect of Two Anti-Sperm Monoclonal Antibodies, Administered Singly and in Combination, in the Mouse

  • Patricia M. Saling
  • Paula C. Morton
  • Robert Waibel
Part of the Reproductive Biology book series (RBIO)


Although many reports in the literature suggest an association between infertility and anti-sperm antibodies, the particular sperm antigens) that may be responsible is unknown. In an effort to sort out this dilemma, as well as to identify sperm components involved specifically at discrete steps of the gamete interaction process, we have generated antimouse sperm monoclonal antibodies (mAbs).


Passive Immunization Tubal Lumen Mouse Sperm Contraceptive Vaccine Sperm Penetration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Biggers, J. D., 1969, Are there general principles of contraceptive technology? Proc. Pakistan International Family Planning Conference at Dacca, Jan. 28–Feb. 4, 1969. pp. 283–288.Google Scholar
  2. Biggers, J. D., 1976, General principles of contraceptive technology. IN: “Regulation of Human Fertility.” K. S. Moghissi and T. N. Evans, eds. Wayne State University Press: Detroit. pp. 28–56.Google Scholar
  3. Goding, J. W., 1983, “Monoclonal Antibodies: Principles and Practice.” Academic Press: New York.Google Scholar
  4. Goldberg, E., 1983, Current status of research on sperm antigens: Potential application as contraceptive vaccines. IN: “Research Frontiers in Fertility Regulation.” Zatuchni, G.I., ed. PARFR, Chicago, Ill. Vol. 2, No.6.Google Scholar
  5. Goldberg, E., T. E. Wheat, and V. Gonzales-Prevatt, 1983, Development of a contraceptive vaccine based on synthetic antigenic determinants of lactate dehydrogenase-C4. IN: “Immunology of Reproduction.” T. G. Wegman and T. J. Gill, eds. Oxford University Press: New York, pp. 491–504.Google Scholar
  6. Hunter, W. M. and F. C. Greenwood, 1962, Preparation of iodine-131 labelled growth hormone of high specific activity. Nature 194: 495–496.PubMedCrossRefGoogle Scholar
  7. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall, 1951, Protein measurement with the Folin Phenol reagent. J. Biol. Chem. 193: 265–275.PubMedGoogle Scholar
  8. Saling, P. M. and K. A. Lakoski, 1985, Mouse sperm antigens that participate in fertilization. II. Inhibition of sperm penetration through the zona pellucida using monoclonal antibodies. Biol. Reprod., in press.Google Scholar
  9. Saling, P. M. and R. Waibel, 1985, Mouse sperm antigens that participate in fertilization. III. Passive immunization with a single monoclonal anti-sperm antibody inhibits pregnancy and fertilization in vivo. Biol. Reprod., in press.Google Scholar
  10. Saling, P. M., L. M. Raines, and M. G. O’Rand, 1983, Monoclonal antibody against mouse sperm blocks a specific event in the fertilization process. J. Exp. Zool. 227: 481–486.PubMedCrossRefGoogle Scholar
  11. Saling, P. M., G. P. Irons, and R. Waibel, 1985a, Mouse sperm antigens that participate in fertilization. I. Inhibition of sperm fusion with the egg plasma membrane using monoclonal antibodies. Biol. Reprod., in press.Google Scholar
  12. Saling, P. M., R. Waibel, and K. A. Lakoski, 1985b, Immunological identification of sperm antigens that participate in fertilization. IN: “The Molecular and Cellular Biology of Fertilization.” J. L. Hedrick, ed. Plenum Press: New York, NY, in press.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Patricia M. Saling
    • 1
  • Paula C. Morton
    • 1
  • Robert Waibel
    • 1
  1. 1.Department of Obstetrics and GynecologyDuke University Medical CenterDurhamUSA

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