Cloning and Expression of Biologically Active Human Fertility Hormones

  • Vemuri B. Reddy
  • Anton K. Beck
  • Vincent Vellucci
  • Anthony J. Garramone
  • Edward G. Bernstine
Part of the Reproductive Biology book series (RBIO)


The three human gonadotropins, choriogonadotropin (hCG), luteinizing hormone (hLH) and follicle stimulating hormone (hFSH), are glycoproteins playing a major role in ovulation, spermatogenesis and conception. hCG is secreted by the developing placenta during the first trimester of pregnancy. Both hLH and hFSH are secreted by the pituitary and together play a central role in regulating menstrual cycle and ovulation.


BeWo Cell Klenow Polymerase SV40 Late Promoter 
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  1. 1.
    Canfield, R.E., Birkens, S., Morse, J.H. and Morgan, F.J. (1976) in Peptide Hormones, ed. Parsons, J.A. (Univ. Park Press, Baltimore), pp. 299–315.Google Scholar
  2. 2.
    Pierce, J.G. and Parsons, T.F.A. (1981) Annu. Rev. Biochem. 50, 465–495.PubMedCrossRefGoogle Scholar
  3. 3.
    Kessler, M., Reddy, M.S., Shah, R.H. and Bahl, O.P. (1979) J. Biol. Chem. 254, 7901–7908.PubMedGoogle Scholar
  4. 4.
    Kessler, M.J., Mise, T., Ghai, R.D. and Bahl, O.P. (1979) J. Biol. Chem. 254, 7909–7914.PubMedGoogle Scholar
  5. 5.
    McKerns, K.W. (1978) Structure and Function of the Gonadotropins (Plenum Press, New York and London).Google Scholar
  6. 6.
    Hamer, D.H. and Leder, P. (1979) Nature (London) 281, 35–40.CrossRefGoogle Scholar
  7. 7.
    Gething, M.J. and Sambrook, J. (1981) Nature (London) 293, 620–625.CrossRefGoogle Scholar
  8. 8.
    Gluzman, Y. (1982) Eukaryotic Vectors (Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.).Google Scholar
  9. 9.
    Gluzman, Y. (1981) Cell, 23, 175–182.PubMedCrossRefGoogle Scholar
  10. 10.
    Maxam, A. and Gilbert, W. (1980) Methods Enzymol. 65, 499–599.PubMedCrossRefGoogle Scholar
  11. 11.
    Fiddes, J.C. and Goodman, H.M. (1979) Nature (London) 281, 351–356.CrossRefGoogle Scholar
  12. 12.
    Fiddes, J.C. and Goodman, H.M. (1980) Nature (London) 286, 684–687.CrossRefGoogle Scholar
  13. 13.
    Talmadge, K., Vamvakopoulos, N.C. and Fiddes, J.C. (1984) Nature (London) 307, 37–40.CrossRefGoogle Scholar
  14. 14.
    Maniatis, T., Hardison, R.C., Lacy, E., Laurer, J., O’Connell, C., Quon, D., Sim, G.K. and Efstrantiadis, A. (1978) Cell, 15, 687–701.PubMedCrossRefGoogle Scholar
  15. 15.
    Sompayrac, L.M. and Danna, K.J. (1981) Proc. Natl. Acad. Sci., USA 78, 7575–7578.PubMedCrossRefGoogle Scholar
  16. 16.
    Kimura, G. and Dulbecco, R. (1972) Virology 49, 79–81.CrossRefGoogle Scholar
  17. 17.
    Loraine, J.A. and Brown, J.B. (1959) J. Endocrinol. 18, 77–84.PubMedCrossRefGoogle Scholar
  18. 18.
    Laemmli, U.K. (1970) Nature (London) 227, 680–685.CrossRefGoogle Scholar
  19. 19.
    Patillo, R.A. and Grey, G.O. (1968) Cancer Res. 28, 1231–1236.Google Scholar
  20. 20.
    Penman, S. (1966) J. Mol. Biol. 17, 117–130.PubMedCrossRefGoogle Scholar
  21. 21.
    Maniatis, T. Fritsch, E.F. and Sambrook, J. (1982) Molecular cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.).Google Scholar
  22. 22.
    Bhaskara Reddy, V., Ghosh, P.K., Lebowitz, P. and Weissman, S.M. (1978) Nucleic Acids Res. 5, 4195–4213.CrossRefGoogle Scholar
  23. 23.
    Reddy, V.B., Thimmappaya, B., Dhar, R., Subramanian, K.N., Zain, B.S., Pam, J., Ghosh, P.K., Celma, M.L. and Weissman, S. (1978) Science 200, 494–502.PubMedCrossRefGoogle Scholar
  24. 24.
    Reddy, V.B., Beck, A.K., Garramone, A.J., Vellucci, V., Lustbader, J. and Bernstine, E.G. (1985) Proc. Natl. Acad. Sci., U.S.A. 82, 3644–3648.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Vemuri B. Reddy
    • 1
  • Anton K. Beck
    • 1
  • Vincent Vellucci
    • 1
  • Anthony J. Garramone
    • 1
  • Edward G. Bernstine
    • 1
  1. 1.Integrated Genetics, Inc.FraminghamUSA

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