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The T-DNA Genes of Agrobacterium Plasmids Appear to be of a Complex Evolutionary Origin

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Book cover Genetics, Development, and Evolution

Part of the book series: Stadler Genetics Symposia Series ((SGSS))

Abstract

Crown galls are typical neoplasmic tissues of plants. These plant tumours are the result of a complex interaction between susceptible plants and gram-negative bacteria (Agrobacterium turnefaciens). During this interaction a defined segment (T-DNA) of a large extrachromosomal plasmid harbored by Agrobacteria (Ti-plasmid) is transferred from the bacteria to the nucleus of infected plant cells. The transferred DNA segment is stably integrated in one of many possible loci on different chromosomes (for recent reviews see Caplan et al., 1983; Gheysen et al., 1985; Nester et al., 1984; Helle et al., 1984).

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References

  • Akiyoshi, D. E., Regier, D. A., Jen, G., and Gordon, M. P., 1985, cloning and nucleotide sequence of the tzs gene from Agrobacterium tumefaciens strain T37, Nucleic Acids Res., 13:2773.

    Article  PubMed  CAS  Google Scholar 

  • Akiyoshi, D. E., Klee, H., Amasino, R. M., Nester, E. W., and Gordon, M. P., 1984, T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis, Proc. Natl. Acad. Sci. USA, 81:5994, 13:2773.

    Article  PubMed  CAS  Google Scholar 

  • Akiyoshi, D. E., Morris, R. O., Hinz, R., Mischke, B. S., Kosuge, T., Garfinkel, D. J., Gordon, M. P., and Nester, W., 1983, Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA, Proc. Natl. Acad. Sci. USA, 80:407.

    Article  PubMed  CAS  Google Scholar 

  • Barry, G. F., Rogers, S. G., Fraley, R. T., and Brand, L., 1984, Identification of a cloned cytokinin biosynthetic gene, Proc. Natl. Acad. USA, 81:4776.

    Article  CAS  Google Scholar 

  • Buchmann, I., Marner, F.-J., Schröder, G., Waffenschmidt, S., and Schröder, J., 1985, Tumor genes in plants: T-DNA encoded cytokinin biosynthesis, EMBO J., 4:853.

    PubMed  CAS  Google Scholar 

  • Caplan, A., Herrera, Estrella, L., Inzá, D., Van Haute, E., Van Montagu, M., Schell, J., and Zambryski, P., 1983, Introduction of genetic material into plant cells. Science, 222:815.

    Article  PubMed  CAS  Google Scholar 

  • Chapman, R. W., and Morris, R. O., 1977, Trans-zeatin in culture filtrates of Agrobacterium turnefaciens, Biochem, Biophys. Res. Commun., 76:453–459.

    Article  Google Scholar 

  • Cherayil, J. D., and Lipsett, M. N., 1977, Zeatin ribonucleotides in the transfer ribonucleic acid of Rhizobium leguminosarum, Agrobacterium tumefaciens, Corynebacterium fascians and Erwinai amylovora, J, Bacteriol., 131:741.

    CAS  Google Scholar 

  • Claeys, M., Messens, E., Van Montagu, M., and Schell, J., 1978, Quantitative determination of cytokinins in culture media of Agrobacterium tumefaciens, Quant. Mass Spec. Lift Sci., 2:409.

    Google Scholar 

  • Comai, L., and Kosuge, T., 1980, Involvement of plasmid deoxyribonucleic acid in indeoleacetic acid synthesis in Pseudomonas sayastanoi, J. Bacteriol., 143:950.

    PubMed  CAS  Google Scholar 

  • De Greve, H., Dhaese, P., Seurinck, J., Lemmers, M., Van Montagu, M., and Schell, J., 1982, Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene, J. Mol. Appl. Genet., 1:499.

    PubMed  Google Scholar 

  • Depicker, A., Stachel, S., Dhaese, P., Zambryski, P., and Goodman, H. M., 1982, Nopaline synthase: transcript mapping and DNA sequence. J. Mol. Appl. Genet. 1:561

    PubMed  CAS  Google Scholar 

  • Dhaese, P., De Greve, H., Gielen, J., Seurinck, J., Van Montagu, M., and Schell, J., 1983, Identification of sequences involved in the polyadenylation of higher plant nuclear transcripts using Agrobacterium T-DNA genes as models, EMBO J., 2: 419.

    PubMed  CAS  Google Scholar 

  • Engler, G., Depicker, A., Maenhaut, R., Villarroel-Mandiola, R., Van Montagu, M. Schell, J. 1981, Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti-plasmid of Agrobacterium tumefaciens, J. Mol. Biol., 152:183.

    Article  PubMed  CAS  Google Scholar 

  • Follin, A., Inzé, D., Budar, F., Genetello, C., Van Montagu, M., and Schell, J., 1985, Genetic evidence that the tryptophan 2-mono-oxygenase gene of Pseudomonas savastanoi is functionally equivalent to one of the T-DNA genes involved in plant tumour formation by Agrobacterium tumefaciens, Mol. Gen. Genet., in press.

    Google Scholar 

  • Garfinkel, D. J., Simpson, R. B., Ream, L. W., White, F. F., Gordon, M. P., and Nester, E. W., 1981, Genetic analysis

    Google Scholar 

  • Gordon, M. P., and Nester, E. W., 1981, Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis, Cell, 27:143.

    Article  PubMed  Google Scholar 

  • Gheysen, G., Dhaese, P., Van Montagu, M., and Schell, J., 1985, DNA flux across genetic barriers: the crown gall phenomenon, in: “Genetic Flux in Plants” (Advances in Plant Gene Research, vol. 2), B. Hohn, and E. S. Dennis, eds., Springer Verlag, Wien, pp. 11–47.

    Chapter  Google Scholar 

  • Gielen, J., De Beuckeleer, M., Seurinck, J., Deboeck, F., De Greve, H., Lemmers, M., Van Montagu, M., and Schell, J., 1984, The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAchS, EMBO J., 3:835.

    PubMed  CAS  Google Scholar 

  • Goldberg, S. B., Flick, J. S., and Rogers, S. G., 1984, Nucleotide sequence of the tmr locus of Agrobacterium tumefaciens pTiT37 T-DNA, Nucl. Acids Res., 12:4665.

    Article  PubMed  CAS  Google Scholar 

  • Heidekamp, F., Dirkse, W. G., Hille, J., and Van Ormondt, H., 1983, Nucleotide sequence of the Agrobacterium tumefaciens octo-pine Ti-plasmid-encoded tmr gene, Nucl. Acids Res., 11:6211.

    Article  PubMed  CAS  Google Scholar 

  • Hille, J., Hoekema, A., Hooykaas, P., and Schilperoort, R. A., 1984, Gene organisation of the Ti-plasmid, in: “Genes Involved in Microbe-Plant Interactions” (Advances in Plant Gene Research, Vol. 1), D.P.S. Verma, and Th. Hohn, eds., Springer, Wien, pp. 287–309.

    Chapter  Google Scholar 

  • Inzé, D., Follin, A., Van Lijsebettens, M., Simoens, C., Genetello, C., Van Montagu, M., and Schell, J., 1984, Genetic analysis of the individual T-DNA genes of Agrobacterium tumefaciens; further evidence that two genes are involved in indole-3-acetic acid synthesis, Mol. Gen. Genet., 194:265.

    Article  Google Scholar 

  • Joos, H., Inzé, D., Caplan, A., Sormann, M., Van Montagu, M., and Schell, J., 1983, Genetic analysis of T-DNA transcripts in nopaline crown galls, Cell, 32:1057

    Article  PubMed  CAS  Google Scholar 

  • Kao, J. C., Perry, K. L., and Kado, C. I., 1982, Indoleacetic acid complementation and its relation to host range specifying genes on the Ti plasmid of Agrobacterium tumefaciens, Mol. Gen. Genet., 188:425.

    Article  PubMed  CAS  Google Scholar 

  • Kemper, E., Waffenschmidt, S., Weiler, E. W., Rausch, T., and Schröder, J., 1985, T-DNA-encoded auxin formation in crown-gall cells, Planta, 163:257.

    Article  CAS  Google Scholar 

  • Kosuge, T., Heskett, M. G., and Wilson, E. E., 1966, Microbial synthesis and degradation of indole-3-acetic acid. The conversion of L-tryptophan to indole-3-acetamide by an enzyme system for Pseudomonas savastanoi, J. Biol. Chem., 241:3738.

    PubMed  CAS  Google Scholar 

  • Leemans, J., Deblaere, R., Willmitzer, L., De Greve, H., Hernalsteens, J.-P., Van Montagu, M., and Schell, J., 1982, Genetic identification of functions of TL-DNA transcripts in octopine crown galls, EMBO J., 1:147.

    PubMed  CAS  Google Scholar 

  • Morris, R. O., Akiyoshi, D. E., MacDonald, E.M.S., Morris, J. W., Regier, D. A., and Zaerr, J. B., 1982, Cytokinin metabolism in relation to tumor induction by Agrobacterium tumefaciens, in: “Plant Growth Substances 1982”, P. F. Wareing, ed., Academic Press, New York, pp. 175–183.

    Google Scholar 

  • Murai, N., Skoog, F., Doyle, M. E., and Hanson, R. S., 1980, Relationship between cytokinin production, presence of Plasmids and fasciation caused by strains of C . fascians, Proc. Natl. Acad. Sci. USA, 77:619.

    Article  PubMed  CAS  Google Scholar 

  • Nester, E. W., Gordon, M. P., Amasino, R. M., and Yanofsky, M. F., 1984, Crown gall: a molecular and physiological analysis, Ann. Rev. Plant Physiol., 35:387.

    Article  CAS  Google Scholar 

  • Ooms, G., Hooykaas, P. J., Moleman, G., and Schilperoort, R. A., 1981, Crown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octo-pine Ti plasmids; analysis of T-DNA functions, Gene, 14:33.

    Article  PubMed  CAS  Google Scholar 

  • Regier, D. A., and Morris, R. O., 1980, Secretion of trans-zeatin by Agrobacterium tumefaciens: a function determined by the nopaline Ti plasmid, Biochem. Biophys. Res. Commun., 104:1560.

    Google Scholar 

  • Schröder, G., Klipp, W., Hillebrand, A., Ehring, R., Koncz, C., and Schröder, J., 1983, The conserved part of the T-region in Ti-plasmids expresses four proteins in bacteria, EMBO J., 2:403.

    PubMed  Google Scholar 

  • Schröder, G., Waffenschmidt, S., Weiler, E. W., and Schröder, J., 1984, The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur. J. Biochem., 138:387.

    Article  PubMed  Google Scholar 

  • Smidt, M., and Kosuge, T., 1978, The role of indole-3-acetic acid accumulation by alpha methyl tryptophan-resistant mutants of Pseudomonas savastanoi in gall formation on oleanders Physiol.Plant Pathol., 13:203.

    CAS  Google Scholar 

  • Surico, G., Comai, L., and Kosuge, T., 1975, Pathogenicity of strains of Pseudomonas syringae pv. savastanoi and their indoleacetic acid deficient mutants on olive and oleander, Phytopathology, 74:490.

    Article  Google Scholar 

  • Taya, Y., Tanaka, Y., and Nishimura, S., 1978, 5′-AMP is a direct precursor of cytokinin in Dictyostelium discoideum, Nature, 271:545.

    Article  PubMed  CAS  Google Scholar 

  • Thomashow, L. S., Reeves, S., and Thomashow, M. F., 1984, Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti plasmid pTiA6 encodes an enzyme that catalyzes synthesis of indoleacetic acid, Proc. Natl. Acad. Sci. USA, 81:5071.

    Article  PubMed  CAS  Google Scholar 

  • Van Onckelen, H., Rüdelscheim, P., Inzé, D., Follin, A., Messens, E., Horemans, S., Schell, J., Van Montagu, M., and De Greef, J., 1985, Tobacco plants transformed with the Agrobacterium T-DNA gene 1 contain high amounts of indole-3-acetamide, FEBS Letters, 181:373.

    Article  Google Scholar 

  • Weiler, E. W., and Spanier, K., 1981, Phytohormones in the formation of crown gall tumors, Planta, 153:326.

    Article  CAS  Google Scholar 

  • Willmitzer, L., Dhaese, P., Schreier, P. H., Schmalenbach, W., Van Montagu, M., and Schell, J., 1983, Size, localisation, and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; evidence for common transcripts present in both octopine and nopaline tumors, Cell, 32:1045.

    Article  PubMed  CAS  Google Scholar 

  • Willmitzer, L., Simons, G., and Schell, J., 1982, The TL-DNA in octopine crown gall tumours codes for seven well-defined polyadenylated transcripts, EMBO J., 1:139.

    PubMed  CAS  Google Scholar 

  • Willmitzer, L., Schmalenbach, W., and Schell, J., 1981, Transcription of T-DNA in octopine and nopaline crown gall tumours is inhibited by low concentrations of alpha-amanitin, Nucl. Acids Res., 9:4801.

    Article  PubMed  CAS  Google Scholar 

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© 1986 Plenum Press, New York

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Schell, J. (1986). The T-DNA Genes of Agrobacterium Plasmids Appear to be of a Complex Evolutionary Origin. In: Gustafson, J.P., Stebbins, G.L., Ayala, F.J. (eds) Genetics, Development, and Evolution. Stadler Genetics Symposia Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5137-5_15

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  • DOI: https://doi.org/10.1007/978-1-4684-5137-5_15

  • Publisher Name: Springer, Boston, MA

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