Abstract
Transposons and transposon- and retrovirus-like DNA segments form a major part of the repetitive DNA in many eukaryotes. They are a cause of many “spontaneous” mutations in Drosophila and other organisms, and many catalyze the rearrangements of large segments of DNA that have occurred during evolution (Spradling and Rubin, 1981; Kleckner, 1981; Flavell et al., 1981; Roeder and Fink 1983; Fedoroff, 1983). In the course of cloning segments of Dictvostelium discoideum genomic DNA that are expressed preferentially during early differentiation, we isolated the DIRS-1 transposon (Chung et al., 1983; Zuker and Lodish, 1981). DIRS-1 is unusual in that its transcription is induced either by heat shock or by the high cell density that occurs during the early stages of differentiation. It is also novel in that its structure is unlike any transposon in prokaryotes or eukaryotes that has been identified to date. DIRS-1 has been independently isolated by Rosen et al. (1983) who call it Tdd-1.
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References
Cappello, J., Cohen, S. M., and Lodish, H. F., 1984a, Dictyostelium transposable element DIRS-1 preferentially inserts into DIRS-1 sequences, Mol. Cell. Biol., 4:2207–2213.
Cappello, J., Zuker, C., and Lodish, H. F. 1984b, Repetitive Dictyostelium heat-shock promotor functions in Saccharomyces cerevisiae, Mol. Cell. Biol., 4:591–598.
Chung, S., Zuker, C., and Lodish, H. F., 1983, A repetitive and apparently transposable DNA sequence in Dictyostelium discoideum associated with developmentally regulated RNAs, Nucl. Acids Res. 11:4835–4852.
Cohen, S. M., Cappello, J., and Lodish, H. F., 1984, Transcription of Dictyostelium discoideum transposable element DIRS-1, Mol. Cell. Biol., 4:2332–2340.
Del Rey, F. J., Donahue, T. F., and Fink, G. R., 1982, Sigma, a repetitive element found adjacent to tRNA genes of yeast, Proc. Nal. Acad. Sci. USA., 79:4138–4142
Eibel, H., Gafner, J., Stotz, A., and Philippsen, P., 1980, Characterization of the yeast mobile element Tyl, Cold Spring Harbor Symp. Quant. Biol., 45:609-
Falkenthal, S., Graham, M. L., Korn, E. L., and Lengyel, J. A., 1981, Transcription, processing and turnover from the Drosophila mobile genetic element copia, Devel. Biol., 92:294-
Fedoroff, N. V. 1983, Controlling elements in cell lineages, in: “Mobile Genetic Elements,” J. A. Shapiro, ed., Academic Press, New York, pp. 1–57.
Flanell, R. B., O’Dell, M., and Hutchinson, J., 1981, Nucleotide sequence organization in plant chromosomes and evidence for sequence translocation during evolution, Cold Spring Harbor Svmp. Quant. Biol., 45:501-
Kimmel, A. R., and Firtel, R. A., 1983, Sequence organization in Dictyostelium: unique structure at the 5′ ends of protein coding genes, Nucl. Acids Res., 11:541–552.
Kleckner, N., 1981, Transposable elements in prokaryotes, Annu. Rev. Genet.. 15:341-
Majors, J. E., Swanstrom, R., DeLorre, W. J., Payne, G. S., Hughes, S. H., Ortiz, S., Quintrell, N., Bishop, J. M., and Varmus, H. E., 1980, DNA intermediates in the replication of retroviruses are structurally (and perhaps functionally) related to transposable elements, Cold Spring Harbor Symp. Quant. Biol., 45:731-
Mangiarotti, G., Zuker, C., Chisholm, R., and Lodish, H. F., 1983, Different mRNAs have different nuclear transit times in Dictyostelium discoideum aggregates, Mol. Cell. Biol., 3:1151–1517.
Mangiarotti, G., Lefebvre, P., and Lodish, H. F., 1982, Differences in the stability of development tally regulated mRNAs in aggregated and disaggregated Dictvostelium discoideum cells, Devel. Biol., 89:82–91.
Murphy, E., and Lofdahl, S., 1984, Transposition of Tn554 does not generate a target duplication, Nature (London), 307:292–294.
O’Hare, K., and Rubin, G. M., 1983, Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanocraster genome, Cell, 34:25–35.
Pelham, H. R. B., 1982, A regulatory upstream promoter element in the Drosophila HSP 70 heat shock gene, Cell. 30:517–528.
Pelham, H.R. B., and Bienz, M., 1982, A synthetic heat-shock promoter confers heat inducibility on the herpes simplex thymidine kinase gene, EMBO J., 1:1473-
Potter, S. S., 1982, DNA sequence of a foldback transposabale element in Drosophila, Nature (London), 297:201–204.
Roeder, G. S., and Fink, G. R., 1983, Transposable elements in yeast, in: “Mobile Genetic Elements,” J. A. Shapiro, ed., Academic Press, New York, pp. 250–326.
Rosen, E., Sivertsen, A., and Firtel, R. A., 1983, An unusual transposon encoding heat shock inducible and developmentally regulated transcripts in Dictyostelium, Cell, 35:243-
Rosenzweig, B., Liao, L. W., and Hirsh, D., 1983, Target sequences for the C. elegans transposable element Tcl, Nucl. Acids Res., 11:7137–7140.
Spradling, A. C., and Rubin, G. M., 1981, Drosophila genome organization: conserved and dynamic aspects, Annu. Rev. Genet., 15:219-
Stanfield, S. W., and Lengyel, J. A., 1980, Small circular deoxyribonucleic acid of Drosophila melanogaster: homologous transcripts in the nucleus and cytoplasm, Biochemistry, 19:3873-.
Yvan, R., 1981, Structure and mechanism of multifunctional restriction endonucleases, Annu. Rev. Biochem.. 50:285-
Zuker, C., 1983, Repetitive developmentally regulated genes in Dictyostelium, Ph.D. Thesis, Massachusetts Institute of Technology, Cambridge, MA.
Zuker, C., Cappello, J., Chisholm, R. L., and Lodish, H. F., 1983, A repetitive Dictyostelium gene family that is induced during differentiation and by heat shock, Cell, 34:997–1005.
Zuker, C., Cappello, J., Lodish, H. F., George, P., and Chung, S., 1984, Dictyostelium transposable element DIRS-1 has 350-base-pair inverted terminal repeats that contain a heat shock promoter, Proc. Natl. Acad. Sci. USA, 81:2660–2664.
Zuker, C., and Lodish, H. F., 1981, Repetitive sequences cotranscribed with developmentally regulated Dictyostelium discoideum mRNAs, Proc. Natl. Acad. Sci. USA, 78:5386–5390.
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© 1986 Plenum Press, New York
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Cappello, J., Cohen, S.M., Handelsman, K., Lodish, H.F. (1986). Structure and Regulated Transcription of DIRS-1, A Novel Dictyostelium Discoideum Transposable Element. In: Gustafson, J.P., Stebbins, G.L., Ayala, F.J. (eds) Genetics, Development, and Evolution. Stadler Genetics Symposia Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5137-5_11
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DOI: https://doi.org/10.1007/978-1-4684-5137-5_11
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