Skip to main content
SpringerLink
Log in

Hormonal Receptor Plasticity in the Brain as Shown by In Vitro Quantitative Autoradiography

  • Chapter
Neuroendocrine Molecular Biology

Part of the book series: Biochemical Endocrinology ((BIOEND))

Abstract

Binding of neuroactive substances to specific receptor sites initiates sophisticated events which result in specific pharmacological, behavioral and physiological responses. The complex organization and the tissular heterogeneity of most of the endocrine organs have led to the development of new methodologies for the precise localization of these binding sites. Together with constant progress obtained for the biochemical characterization of those binding sites by conventional binding assays (mainly carried out on membrane preparations), recent autoradiographic techniques provide us with new interpretations about the numerous interactions underlying brain functions.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Baskin, D.G., Petracca, F., and Dorsa, D.M. 1983. Autoradiographic localization of specific binding sites for 3H-Arg8 vasopressin in the septum of the rat brain with tritium-sensitive film. Eur. J. Pharmac. 90: 155–157.

    Article  CAS  Google Scholar 

  • Baumgarten, H.G., Jenner, S., and Schlossberger, H.G. 1979. Serotonin neurotoxins: Effects of drugs on the destruction of brain serotoninergic, noradrenergic and dopaminergic axons in adult rat by intraventricularly, intracisternally or intracerebrally administered 5,7-dihydroxytryptamine and related compounds. In Neurotoxin, Fundamental and Clinical Advances, I.W. Chubb, L.B. Geffen eds, Adelaide University Union Press, Adelaide, pp. 221–226.

    Google Scholar 

  • Biegon, A., Bercowitz, H., and Samuel D. 1980. Serotonin receptor concentration during the estrous cycle of the rat. Brain Res. 187: 221–225.

    Article  PubMed  CAS  Google Scholar 

  • Biegon, A., Rainbow, T.C., and Mc Ewen, B.S. 1982. Quantitative autoradiography of serotonin receptors in the rat brain. Brain Res. 242: 197–204.

    Article  PubMed  CAS  Google Scholar 

  • Biegon, A., Terlou, M., Voorhuis, T.D., and De Kloet, E.R. 1984. Arginine-vasopressin binding sites in rat brain: A quantitative autoradiographic study. Neuroscience Lett. 44: 229–234.

    Article  CAS  Google Scholar 

  • Bosler, O., and Beaudet, A. 1985. VIP neurons as prime synaptic targets for serotoninergic afferents in rat suprachiasmatic nucleus: a combined radioimmunological and immunocytochemical study. J. Cytol., in press.

    Google Scholar 

  • Brunello, N., Barbacria, M.L., Chuang, D.M., and Costa, E. 1982. Down-regulation of beta adrenergic receptors following repeated desmethyl-imipramine injections: permissive role of serotoninergic axons. Neuropharmacol. 21: 1145–1149.

    Article  CAS  Google Scholar 

  • De Kloet, E.R., Kovacs, G.L., Szabo, G., Telegdy, G., Bohus, B., and Versteeg, D.H.G. 1982. Decreased serotonin turnover in the dorsal hippocampus of rat brain shortly after adrenalectomy: selective normalization after corticosterone substitution. Brain Res. 239: 659–663.

    Article  PubMed  Google Scholar 

  • Fuxe, K., Agnati, L.F., Benfenati, F., Celani, M., Zini, I., Zoli, M., and Mutt, V. 1983. Evidence for the existence of receptor-receptor interactions of monoamine receptors by neuropeptides. J. Neural Transm. suppl. 18: 165–179.

    CAS  Google Scholar 

  • Govoni, S., Hong, J.S., Yang, H.Y.T., and Costa, E. 1980. Increase of neurotensin content elicited by neuroleptics in nucleus accumbens. J. Pharmacol, Exp. Ther. 215: 413–417.

    CAS  Google Scholar 

  • Gozlan, H., El Mestikawy, S., Pichat, L., Glowinski, J. and Hamon, M. 1983. Identification of presynaptic serotonin autoreceptors using a new ligand: 3H-PAT. Nature 305: 140–142.

    Article  PubMed  CAS  Google Scholar 

  • Hervé, D., Tassin, J.P., Studier, J.M., Dana, C., Kitabgi, P., Vincent, J.P., Glowinski, J., and Rostène, W. 1985. Dopaminergic control of 125I-neurotensin binding site density in corticolimbic structures of the rat brain. Proc. Natl. Acad. Sci. USA, in press

    Google Scholar 

  • Héry, M., Faudon, M. and Héry, F. 1984. Effect of VIP on serotonin release in the suprachiasmatic area of the rat: Modulation by estradiol. Peptides 5: 313–317.

    Article  PubMed  Google Scholar 

  • Hoover, D.B., Hancock, J.C. 1983. Autoradiographic localization of quinuclidinyl benzilate binding to rat pituitary gland. Neuroendocrinology 37: 297–301.

    Article  PubMed  CAS  Google Scholar 

  • Hruska, R.E., and Silbergeld, E.K. 1980. Estrogen treatment enhances dopamine receptor sensitivity in the rat striatum. Eur. J. Pharmacol. 61: 397–400.

    Article  PubMed  CAS  Google Scholar 

  • Israel, A., Correa, F.M.A., Niwa, M., Saavedra, J.M. 1984. Quantitative determination of angiotensin II binding sites in rat brain and pituitary gland by autoradiography. Brain Res. 322: 341–345.

    Article  PubMed  CAS  Google Scholar 

  • Kiss, J., Léranth, Cs, and Halasz, B. 1984. Serotoninergic ending on VIP-neurons in the suprachiasmatic nucleus and on ACTH-neurons in the arcuate nucleus of the rat hypothalamus. A combination of high resolution autoradiography and electron microscopic immunocytochemistry. Neuroscience Lett. 44: 119–124.

    Article  CAS  Google Scholar 

  • Lightman, S.L., Ninkovic, M., Hunt, S.P., and Iversen L.L. 1983. Evidence for opiate receptors on pituicytes. Nature 305: 235–237.

    Article  PubMed  CAS  Google Scholar 

  • Mazière, B., Loc’h, C., Hantraye, P., Guillon, R., Duquesnoy, N., Soussaline, F., Naquet, R., Comar, D., and Mazière, M. 1984. 76Brbromospiroperidol: A new tool for quantitative in vivo imaging of neuroleptic receptors. Life Sci. 35: 1349–1356.

    Article  PubMed  Google Scholar 

  • Mc Ewen, B.S., 1982. Glucocorticoids and hippocampus: Receptors in search of a function. In: Current Topics in Neuroendocrinology, D. Ganten, D.W. Pfaff eds, Springer-Verlag, Berlin, pp. 1–22.

    Google Scholar 

  • Meibach, R.C., Maayani, S., and Green J.P. 1980. Characteriezation and radioautography of 3H-LSD by rat brain slices in vitro: the effect of 5-hydroxytryptamine. Eur. J. Pharmacol. 67: 371–382.

    Article  PubMed  CAS  Google Scholar 

  • Morel, G., Leroux, P., and Pelletier, G. 1985. Localization and characterization of somatostatin-14 and somatostatin-28 receptors in the rat pituitary as studied by slide-mounted frozen sections. Neuropeptides 6: 41–52.

    Article  PubMed  CAS  Google Scholar 

  • Nelson, D.L., Herbet, A., Bourgoin, S., Glowinski, J., and Hamon, M. 1978. Characteristics of central 5-HT receptors and their adoptive changes following intracerebral 5,7-dihydroxytryptamine administration in the rat. Mol. Pharmacol. 14: 983–995.

    PubMed  CAS  Google Scholar 

  • Neraeroff, C.B., Youngblood, W.W., Manberg, P.J., Prange, A.J. Jr, and Kizer, J.S. 1983. Regional brain concentrations of neuropeptides in Huntington’s chorea and schizophrenia. Science 221: 972–975.

    Article  Google Scholar 

  • Palacios, J.M., Niehoff, D.L., and Kuhar, M.J. 1981. Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry. Neuroscience Lett. 25: 101–105.

    Article  CAS  Google Scholar 

  • Peroutka, S.J., and Snyder, S.H. 1981. Two distinct serotonin receptors: regional variations in receptor binding in mammalian brain. Brain Res. 208: 339–347.

    Article  PubMed  CAS  Google Scholar 

  • Pert, C.B., Kuhar, M.J., and Snyder, S.H. 1975. Autoradiographic localization of binding of the opiate receptor in rat brain. Life Sci. 16: 1849–1854.

    Article  PubMed  CAS  Google Scholar 

  • Pfaff, D.W. 1968. Autoradiographic localization of radioactivity in rat brain after injection of tritiated sex hormones. Science 161: 1355–1356.

    Article  PubMed  CAS  Google Scholar 

  • Quirion, R. 1983. Interactions between neurotensin and dopamine in the brain: an overview. Peptides 4: 609–615.

    Article  PubMed  CAS  Google Scholar 

  • Rainbow, T.C., Bleisch, W.V., Biegon, A., and Mc Ewen, B.S. 1982. Quantitative densitometry of neurotransmitter receptors. J. Neurosci. Meth. 5: 127–138.

    Article  CAS  Google Scholar 

  • Rainbow, T.C., De Groff, V., Luine, V.N., and Mc Ewen, B.S. 1980. Estradiol 17 beta increases the number of muscarinic receptors in hypothalamic nuclei. Brain Res. 198: 239–243.

    Article  PubMed  CAS  Google Scholar 

  • Reubi, J.C., and Maurer, R. 1985. Visualization of LHRH receptors in the rat brain. Eur. J. Pharmacol. 106: 453–454.

    Article  Google Scholar 

  • Rostène, W.H. 1984. Neurobiological and neuroendocrine functions of the vasoactive intestinal peptide (VIP). Prog. Neurobiology 22: 103–129.

    Article  Google Scholar 

  • Rostène, W., Besson, J., Broer, Y., Dussaillant, M., Grouselle, D., Kitabgi, P., Lhiaubet, A.M., Morgat, J.L., Sarrieau, A., and Vial, M. 1985a. Localisation par radioautographie des récepteurs des neuropeptides dans le système nerveux central. Ann. Endocrinol. 46: 27–33.

    Google Scholar 

  • Rostène, W.H., Fischette, C.T., Dussaillant, M., Mc Ewen, B.S. 1985b. Adrenal steroid modulation of vasoactive intestinal peptide effect on serotonin binding sites in the rat brain shown by in vitro quantitative autoradiography. Neuroendocrinology 40: 129–134.

    Article  PubMed  Google Scholar 

  • Rostène, W.H., Fischette, C.T.,, Rainbow, T.C., and Mc Ewen, B.S. 1983. Modulation by vasoactive intestinal peptide of serotonin receptors in the dorsal hippocampus of the rat brain: An autoradiographic study. Neuroscience Lett. 37: 143–148.

    Article  Google Scholar 

  • Rostène, W.H., Morgat, J.L., Dussaillant, M., Rainbow, T.C., Sarrieau, A., Vial, M., and Rosselin, G. 1984. In vitro biochemical characterization and autoradiographic distribution of H-thyrotropin-releasing hormone binding sites in rat brain sections. Neuroendocrinology 39: 81–86.

    Article  PubMed  Google Scholar 

  • Rostène, W., and Mourre, C. 1985c. Préparation de standards iodés pour radioautographie quantitative in vitro à l’aide d’un film sensible au tritium. C.R. Acad. Sci. Paris 301: 245–250.

    PubMed  Google Scholar 

  • Rostène, W., Quirion, R., Beaudet, A., and Mazière, B. 1986. New brain imaging approaches for the visualization of receptors for chemical messengers and drugs. Médecine Sciences, in press

    Google Scholar 

  • Rotsztejn, W.H., Besson, J., Briaud, B., Gagnant, L., Rosselin, G., and Kordon, C. 1980. Effect of steroids on vasoactive intestinal peptide in discrete brain regions and peripheral tissues. Neuroendocrinology 31: 287–291.

    Article  PubMed  CAS  Google Scholar 

  • Sadoul, J.L., Checler, F., Kitabgi, P., Rostène, W., Javoy-Agid, F. and Vincent, J.P. 1984. Loss of high affinity neurotensin receptors in substantia nigra from Parkinsonian subjects. Biochem. Biophys. Res. Commun. 125: 395–404.

    Article  PubMed  CAS  Google Scholar 

  • Sapolsky, R.M., Krey, L.C., and Mc Ewen, B.S. 1983a. Corticosterone receptors decline in a site-specific manner in the aged rat brain. Brain Res. 289: 235–240.

    Article  PubMed  CAS  Google Scholar 

  • Sapolsky, R.M., Krey, L.C., and Mc Ewen, B.S. 1983b. The adrenocortical stress-response in the aged male rat: Impairment of recovery from stress. Exp. Gerontol. 18: 55–64.

    Article  PubMed  CAS  Google Scholar 

  • Sarrieau, A., Vial, M., Philibert, D. and Rostène, W. 1984a. In vitro autoradiographic localization of H-corticosterone binding sites in rat hippocampus. Eur. J. Pharmacol. 98: 151–152.

    Article  PubMed  CAS  Google Scholar 

  • Sarrieau, A., Vial, M., Philibert, D., Moguilewsky, M., Dussaillant, M., Mc Ewen, B.S., and Rostène, W. 1984b. In vitro binding of tritiated glucocorticoids directly on unfixed rat brain sections. J. Ster. Biochem. 20: 1233–1238.

    Article  CAS  Google Scholar 

  • Sokoloff, L. 1977. Relation between physiological function and energy metabolism in the central nervous system. J. Neurochem. 29: 13–26.

    Article  PubMed  CAS  Google Scholar 

  • Stumpf, W.E. 1968. Estradiol-concentrating neurons: Topography in the hypothalamus by dry-mount autoradiography. Science 162: 1001–1003.

    Article  PubMed  CAS  Google Scholar 

  • Tassin, J.P., Simon, H., Hervé, D., Blanc, G., Le Moal, M., Glowinski, J., and Bockaert, J. 1982. Non-dopaminergic fibres may regulate dopaminesensitive adenylate-cyclase in the prefrontal cortex and nucleus accumbens. Nature 295: 696–698.

    Article  PubMed  CAS  Google Scholar 

  • Uhl, G.R., and Kuhar, M.J. 1984. Chronic neuroleptic treatment enhances neurotensin receptor binding in human and rat substantia nigra. Nature 309: 350–352.

    Article  PubMed  CAS  Google Scholar 

  • Uhl, G.R., Whitehouse, P.J., Price, D.L., Tourtelotte, W.W., and Kuhar, M.J. 1984. Parkinson’s disease: depletion of substantia nigra neurotensin receptors. Brain Res. 308: 186–190.

    Article  PubMed  CAS  Google Scholar 

  • Unnerstall, J.R., Niehoff, D.L., Kuhar, M.J., and Palacios, J.M. 1982. Quantitative receptor autoradiography using H-Ultrofilm: application to multiple benzodiazepine receptors. J Neurosci. Meth. 6: 59–73.

    Article  CAS  Google Scholar 

  • Wagner, H.R., Crutcher, K.A., and Davies, J.N. 1979. Chronic estrogen treatment decreases beta adrenergic responses in rat cerebral cortex. Brain Res. 171: 147–151.

    Article  PubMed  CAS  Google Scholar 

  • Weissmann-Nanopoulos, D., Mach, E., Magre, J., Blaquiere, B., and Pujol, J.F. 1985. Evidence for 5-HT1 binding sites on 5-HT containing neurons in the raphe dorsalis and centralis of the rat brain. Neurochem. Intern., in press

    Google Scholar 

  • Wynn, P.C., Hauger, R.L., Holmes, M.C., Millan, M.A., Catt, K.J., and Aguilera, G. 1984. Brain and pituitary receptors for corticotropin releasing factor: Localization and differential regulation after adrenalectomy. Peptides 5: 1077–1084.

    Article  PubMed  CAS  Google Scholar 

  • Yamamura, H.I., Gee, K.W., Brinton, R.E., Davis, T.P., Mac Hadley, and Wamsley, J.K. 1983. Light microscopic autoradiographic visualization of H-arginine vasopressin binding sites in rat brain. Life Sci. 32: 1919–1924.

    Article  PubMed  CAS  Google Scholar 

  • Young, W.S., III, and Kuhar, M.J. 1980. Serotonin receptor localization in rat brain by light microscopic autoradiography. Eur. J. Pharmacol. 62: 237–239.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. INSERM U. 55, Hôpital Saint-Antoine, 75571, Paris Cedex 12, France

    William H. Rostène, Jocelyne Magre & Alain Sarrieau

  2. INSERM U. 114, Collège de France, 75231, Paris Cédex 05;, France

    Denis Hervé

  3. Centre de Biochimie CNRS, Université de Nice, Parc Valrose, 06034, Nice Cédex, France

    Patrick Kitabgi

Authors
  1. William H. Rostène
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Denis Hervé
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Patrick Kitabgi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jocelyne Magre
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Alain Sarrieau
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. MRC Brain Metabolism Unit, Edinburgh, Scotland

    G. Fink  & A. J. Harmar  & 

  2. The International Foundation for Biochemical Endocrinology, Blue Hill Falls, Maine, USA

    Kenneth W. McKerns

Rights and permissions

Reprints and permissions

Copyright information

© 1986 Plenum Press, New York

About this chapter

Cite this chapter

Rostène, W.H., Hervé, D., Kitabgi, P., Magre, J., Sarrieau, A. (1986). Hormonal Receptor Plasticity in the Brain as Shown by In Vitro Quantitative Autoradiography. In: Fink, G., Harmar, A.J., McKerns, K.W. (eds) Neuroendocrine Molecular Biology. Biochemical Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5131-3_34

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-5131-3_34

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5133-7

  • Online ISBN: 978-1-4684-5131-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation