Abstract
Isolated adult heart cells (myocytes) with properties suitable for metabolic studies are now available in a number of laboratories. These cells are Ca2+-tolerant and show excellent viability and stability. They also retain the electrical properties, responsiveness to hormones and the complex morphological features of heart muscle cells in situ (1–5). Such myocyte preparations should be of considerable value in defining the response of heart cells to ischemia. Myocytes offer a unique opportunity to examine metabolic properties and transport reactions in ventricular muscle cells in the absence of variables due to tissue perfusion and without contributions from vascular cells or other cell types that may be present in intact tissue. In addition, the myocytes can be subjected to uniform perturbations in media of well-defined composition, can be serially sampled to determine the time course of responses, and are amenable to controlled disruption for compartmental analysis (6,7).
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References
G.P. Brierley, C.M. Hohl, and R.A. Altschuld, 1983, Ion movements in adult rat heart myocytes, in: “Myocardial Injury,” J.J. Spitzer, ed., Plenum Pub. Corp., New York, p. 231.
J.W. Dow, N.G.L. Harding, and T. Powell, 1981, Isolated cardiac myocytes I. Preparation of adult myocytes and their homology with intact tissue, Cardiovas. Res. 15: 483.
J.W. Dow, N.G.L. Harding, and T. Powell, 1981, Isolated cardiac myocytes II. Functional aspects of mature cells, Cardiovas. Res. 15: 549.
B.B. Farmer, M. Mancina, E.S. Williams, and A.M. Watanabe, 1983, Isolation of calcium tolerant myocytes from adult rat hearts: Review of the literature and description of a method, Life Sci. 33: 1.
R.L. Kao, E.W. Christman, S.L. Luh, J.M. Kraubs, G.F.O. Tylers, and E.H. Williams, 1980, The effects of insulin and anoxia on the metabolism of isolated mature rat cardiac myocytes, Arch. Biochem. Biophys. 203: 587.
R.A. Altschuld, C. Hohl, A. Ansel, and G.P. Brierley, 1981, Compartmentation of K+ in isolated rat heart cells, Arch. Biochem. Biophys. 209: 175.
C.M. Hohl, A. Ansel, R.A. Altschuld, and G.P. Brierley, 1982, Contracture of isolated rat heart cells on anaerobic to aerobic transition, Am. J. Physiol. 243: H1022.
R.A. Altschuld, J.R. Hostetler, and G.P. Brierley, 1981, Response of isolated rat heart cells to hypoxia, re-oxygenation, and acidosis, Circ. Res. 49: 307.
R.A. Haworth, D.R. Hunter, and H.A. Berkoff, 1981, Contracture in isolated adult rat heart cells, Circ. Res. 49: 1119.
M.P. Murphy, C.M. Hohl, G.P. Brierley, and R.A. Altschuld, 1982, Release of enzymes from adult rat heart myocytes, Circ. Res. 51: 560.
M.A. Russo, A. Cittadini, A.M. Dani, G. Inesi, and T. Terranova, 1981, An ultrastructural study of calcium induced degenerative changes in dissociated heart cells, J. Mol. Cell. Cardiol. 13: 265.
R.A. Altschuld, L. Gibb, A. Ansel, C. Hohl, F.A. Kruger, and Brierley, G.P., 1980, Calcium tolerance of isolated rat heart cells, J. Mol. Cell. Cardiol. 12: 1383.
C.E. Ganote, 1983, Contraction band necrosis and irreversible myocardial injury, J. Mol. Cell. Cardiol. 15: 67.
C.E. Ganote and J.P. Kaltenbach, 1979, Oxygen-induced enzyme release: early events and a proposed mechanism, J. Mol. Cell. Cardiol. 11: 289.
R.B. Jennings and K.A. Reimer, 1981, Lethal myocardial ischemic injury, Am. J. Pathol. 102: 241.
C.M. Hohl, R.A. Altschuld, and G.P. Brierley, 1983, Effects of calcium on the permeability of isolated adult rat heart cells to sodium, Arch. Biochem. Biophys. 221: 197.
P.M. Grinwald and W.G. Naylor, 1981, Calcium entry in the calcium paradox, J. Mol. Cell. Cardiol. 13: 867.
D.J. Hearse, S.M. Humphrey, and G.R. Bullock, 1978, The oxygen paradox and the calcium paradox: Two facets of the same problem?, J. Mol. Cell Cardiol. 10: 641.
D.J. Hearse, S.M. Humphrey, and G.R. Bullock, 1978, The oxygen paradox and the calcium paradox: Two facets of the same problem?, J. Mol. Cell Cardiol. 10: 641.
L.E. Alto and N.S. Dhalla, 1979, Myocardial cation contents during induction of calcium paradox, Am. J. Physiol. 237: H713.
M. Ashraf, 1979, Correlative studies on sarcolemmal ultrastructure, permeability, and loss of intracellular enzymes in the isolated heart perfused with calcium-free medium, Am. J. Pathol. 97: 411.
M. Ashraf, M. Onda, Y. Hirohata, and A. Schwartz, 1982, Therapeutic effect of diltiazem on myocardial cell injury during the calcium paradox, J. Mol. Cell. Cardiol. 14: 323.
R.A. Haworth, D.R. Hunter, H.A. Berkoff and R.L. Moss, 1983, Metabolic cost of the stimulated beating of isolated adult rat heart cells in suspension, Circ. Res. 52: 342.
A. Fabiato, 1983, Calcium-induced release of calcium from the cardiac sarcoplasmic reticulum, Am. J. Physiol. 245: C1.
J.C. Altona and A. van der Laarse, 1982, Anoxia-induced changes in composition and permeability of sarcolemmal membranes in rat heart cell cultures, Cardiovas. Res. 16: 138.
M. Jurkowitz, K.M. Scott, R.A. Altschuld, A.J. Merola, and G.P. Brierley, 1974, Ion transport by heart mitochondria. Retention and loss of energy coupling in aged heart mitochondria, Arch. Biochem. Biophys. 165: 98.
T.Y. Lipskaya, V.D. Templ, L.V. Belousova, E.V. Molokova, and I.V. Rybina, 1981, Investigation of the interaction of mitochondrial creatine kinase with the membrane of the mitochondria, Biochimiya (English Translation) 45: 877.
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© 1986 Plenum Press, New York
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Brierley, G.P., Wenger, W.C., Altschuld, R.A. (1986). Heart Myocytes as Models of the Cellular Response to Ischemia. In: Brautbar, N. (eds) Myocardial and Skeletal Muscle Bioenergetics. Advances in Experimental Medicine and Biology, vol 194. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5107-8_23
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DOI: https://doi.org/10.1007/978-1-4684-5107-8_23
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