Abstract
The primary work of the kidney is active transport.1 It is a long-standing observation that a linear relationship exists between the rate of sodium reabsorption by the whole kidney and its rate of oxygen consumption.2,3 Since the oxygen is consumed at the mitochondria and the energy for active transport is used by the Na,K-ATPase located at the plasma membrane on the basolateral side, a basic question in cellular physiology concerns the mechanism whereby the two processes are linked. The answer to this question leads directly to energy compartmentation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
L. J. Mandel and R. S. Balaban, Stoichiometry and coupling of active transport to oxidative metabolism in epithelial tissues, Am. s1. Physiol. 240: F357 - F371 (1981).
K. Thurau, Renal Na reabsorption and 02 uptake in dogs during hypoxia and hydrocholorothiazide infusion, Proc. Soc. Exp. Biol. Med. 106:714–717 (1961).
G. Torelli, E. Mella, A. Faelli, and S. Costantini, Energy requirements for sodium reabsorption in the in vivo rabbit kidney, AID. J. Physiol. 211: 576–580 (1966).
P. Needleman, J. V. Passonnau, and O. H. Lowry, Distribution of glucose and related metabolites in rat kidney, Aia1 Physiol. 215: 655–659 (1968).
H. A. Lardy and H. Wellman, Oxidative phosphoryla- tions: role of inorganic phosphate and acceptor systems in control of metabolic rates, Biol. Chem. 195: 215–224 (1952).
B. Chance and C. M. Williams, The respiratory chain and oxidative phosphorylation. Adv. Enzymol. Relat. Areas Mol. Biol. 17: 65–134 (1956).
R. S. Balaban, L. J. Mandel. S. Soltoff, and J. M. Storey, Coupling of Na-K-ATPase activity to aerobic respiratory rate in isolated cortical tubules from the rabbit kidney. Proc. Nat’. Acad. $ci. USA 77: 447–451 (1980).
W. E. Jacobus, R. W. Moreadith, and K. M. Vandegaer, Mitochondrial respiratory control. Evidence against the regulation of respiration by extramitochondrial phosphorylation potentials or by {ATP}/{ADP} ratios, J. Biol. Chem. 257: 2397–2402 (1982).
R. L. Veech, J. W. Randolph, N. W. Cornell, and H. A. Krebs, Cytosolic phosphorylation potential, J. Biol. Chem. 254: 6538–6547 (1979).
S. P. Soltoff and L. J. Mandel, Active ion transport in the renal proximal tubule. I. Transport and metabolic studies, I. Sien. Physiol., in press (1984).
T. P. M. Akerboom, H. Bookelman, P. R. Zuurendonk, R. van der Meer, and J. M. Tager, Intramitochondrial and extramitochondrial concentrations of adenine nucleotides and inorganic phosphate in isolated hepatocytes from fasted rats, Eur. J. Biochem. 84: 413–420 (1978).
W. D. Schwenke, S. Soboll, H. J. Seitz, and H. Sies, Mitochondrial and cytosolic ATP/ADP ratios in rat liver in vivo, Biochem. I. 200: 405–408 (1981).
K. F. Lalloue and A. C. Schoolwerth, Metabolic transport in mitochondria, Ann. Rev. Biochem. 48: 871–922 (1979).
R. S. Balaban, Nuclear magnetic resonance studies of epithelial metabolism and function, Fed. Proc. 41: 42–47 (1982).
R. S. Balaban, D. G. Gadian, and G. K. Radda, Phosphorus nuclear magnetic resonance study of the rat kidney in vivo, Kidney Int. 20: 575–579 (1981).
S. P. Soltoff and L. J. Mandel, Active ion transport in the renal proximal tubule. III. The ATP dependence of the sodium pump, J. Gen. Physiol., in press (1984).
P. L. Jorgensen, Regulation of the (Na+ + K+)-activated ATP hydrolyzing enzyme system in rat kidney. I. The effect of adrenalectomy and the supply of sodium on the enzyme system, Biochim. Biophys. Acta 151: 212–224 (1968).
J. M. Braughler and C. N. Corder, Purification of the (Na+ + K4)-adenosine triphosphatase from human renal tissue, Biochim. Biophys. Acta 481: 313–327 (1977).
S. I. Harris, L. Patton, L. Barrett, and L. J. Mandel, (Na+, K+)-ATPase kinetics within the intact renal cell, J. Biol. Chem. 257: 6996–7002 (1982).
S. R. Gullans, P. C. Brazy, S. P. Soltoff, V. W. Dennis, and L. J. Mandel, Metabolic inhibitors: Effects on metabolism and transport in rabbit proximal tubule, Am. J. Physiol. 243: F133 - F140 (1982).
J. Kyte, Immunoferritin determination of the distribution of (Na+ + K+) ATPase over the plasma membranes of renal convoluted tubules. II. Proximal segment, J. Cell. Biol. 68: 304–318 (1976).
R. S. Balaban, S. Soltoff, J. M. Storey, and L. J. Mandel, Improved renal cortical tubule suspension: spectrophotometric study of 02 delivery, Am. J. physiol. 238: F50 - F59 (1980).
P. C. Brazy, S. R. Gullans, L. J. Mandel, and V. W. Dennis, Metabolic requirement for inorganic phosphate by the rabbit proximal tubule. Evidence for a Crabtree effect, J. Clin. Invest. 70: 53–62 (1982).
P. C. Brazy and V. W. Dennis, Characteristics of glucose-phlorizin interactions in isolated proximal tubules, Am. I. Physiol. 234: F279 - F286 (1978).
A. Kleinzeller, J. Kolinska, and I. Benes, Transport of monosaccharides in kidney-cortex cells, Biochem. J. 104: 852–860 (1967).
A. N. Wick, D. R. Drury, H. J. Nakada, and J. B. Wolfe, Localization of the primary metabolic block produced by 2-deoxyglucose, a. Biol. Chem. 224: 963–969 (1957).
P. C. Brazy, L. J. Mandel, S. R. Gullans, and S. P. Soltoff, Interactions between phosphate and oxidative metabolism in proximal renal tubules, AM. J. Physiol., in press (1984).
D. Freeman, S. Bartlett, G. Radda, and B. Ross, Energetics of sodium transport in the kidney. Saturation transfer of P-NMR. Biochim. Biophys. Acta 762:325–336 (1983).
B. Kaissling and W. Kriz, Structural analysis of the rabbit kidney, Advances in Anatomy Embryology and Cell Bioloas 56: 1–123 (1979).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Mandel, L.J., Soltoff, S.P., Brazy, P.C. (1986). Energy Compartmentation and Active Transport in Proximal Kidney Tubules. In: Brautbar, N. (eds) Myocardial and Skeletal Muscle Bioenergetics. Advances in Experimental Medicine and Biology, vol 194. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5107-8_16
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5107-8_16
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5109-2
Online ISBN: 978-1-4684-5107-8
eBook Packages: Springer Book Archive